RU2009125104A - METHOD FOR DETERMINING YKL-40 CHITINASE-LIKE PROTEIN IN HUMAN BIOPATHES AND EXCRETES - Google Patents
METHOD FOR DETERMINING YKL-40 CHITINASE-LIKE PROTEIN IN HUMAN BIOPATHES AND EXCRETES Download PDFInfo
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- RU2009125104A RU2009125104A RU2009125104/15A RU2009125104A RU2009125104A RU 2009125104 A RU2009125104 A RU 2009125104A RU 2009125104/15 A RU2009125104/15 A RU 2009125104/15A RU 2009125104 A RU2009125104 A RU 2009125104A RU 2009125104 A RU2009125104 A RU 2009125104A
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1. Способ иммуноферментного определения концентрации YKL-40, характеризующийся тем, что в лунках планшета сорбируют коллоидный хитин или гепарин, затем в лунки вносят раствор анализируемой пробы (сыворотка, смыв со слизистых носовой полости, бронхо-альвеолярная лаважная жидкость, слезная жидкость), проводят инкубацию для осуществления специфического связывания хитиназо-подобного белка, и после осушения планшета и отмывки в лунки вносят конъюгат фермента с антителами против YKL-40, и после инкубации и отмывки - субстратный буфер, после чего проводят расчет концентрации YKL-40. ! 2. Набор для иммуноферментного определения концентрации YKL-40, характеризующийся тем, что он содержит планшет или стрип, растворы коллоидного хитина или гепарина, конъюгат фермента с антителами против YKL-40, субстратный буфер и стандартные образцы с известными концентрациями YKL-40. 1. An enzyme-linked immunosorbent assay for determining the concentration of YKL-40, characterized in that colloidal chitin or heparin is adsorbed in the wells of the plate, then a solution of the test sample (serum, rinsing from the nasal mucosa, broncho-alveolar lavage fluid, lacrimal fluid) is introduced into the wells an incubation for the specific binding of a chitinase-like protein, and after the plate is dried and washed, the conjugate of the enzyme with anti-YKL-40 antibodies is introduced into the wells, and after incubation and washing, a substrate buffer, after which calculation of lead concentration of YKL-40. ! 2. An enzyme-linked immunosorbent assay for determining the concentration of YKL-40, characterized in that it contains a plate or strip, solutions of colloidal chitin or heparin, an enzyme conjugate with antibodies against YKL-40, a substrate buffer and standard samples with known concentrations of YKL-40.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009125104/15A RU2423707C2 (en) | 2009-06-30 | 2009-06-30 | Method of human biopsy material and excrete analysis for chitinase-like protein ykl-40 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009125104/15A RU2423707C2 (en) | 2009-06-30 | 2009-06-30 | Method of human biopsy material and excrete analysis for chitinase-like protein ykl-40 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2009125104A true RU2009125104A (en) | 2011-01-10 |
| RU2423707C2 RU2423707C2 (en) | 2011-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2009125104/15A RU2423707C2 (en) | 2009-06-30 | 2009-06-30 | Method of human biopsy material and excrete analysis for chitinase-like protein ykl-40 |
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| RU (1) | RU2423707C2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117024586A (en) * | 2023-08-22 | 2023-11-10 | 艾可泰科(浙江)控股有限公司 | Antibody composition and method for detecting concentration of YKL-40 in blood |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2519071C1 (en) * | 2013-04-17 | 2014-06-10 | Федеральное бюджетное учреждение науки "Казанский научно-исследовательский институт эпидемиологии и микробиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека | Highly sensitive method of determining immunoglobulin-protease activity using polymer matrices |
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2009
- 2009-06-30 RU RU2009125104/15A patent/RU2423707C2/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117024586A (en) * | 2023-08-22 | 2023-11-10 | 艾可泰科(浙江)控股有限公司 | Antibody composition and method for detecting concentration of YKL-40 in blood |
| CN117024586B (en) * | 2023-08-22 | 2024-03-08 | 艾可泰科(浙江)控股有限公司 | Antibody composition and method for detecting concentration of YKL-40 in blood |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2423707C2 (en) | 2011-07-10 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20130701 |