RU2009120812A - METHOD FOR PRODUCING ANTIGENIC DRUG FROM BRUCELLA IN L-FORM - Google Patents
METHOD FOR PRODUCING ANTIGENIC DRUG FROM BRUCELLA IN L-FORM Download PDFInfo
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- RU2009120812A RU2009120812A RU2009120812/15A RU2009120812A RU2009120812A RU 2009120812 A RU2009120812 A RU 2009120812A RU 2009120812/15 A RU2009120812/15 A RU 2009120812/15A RU 2009120812 A RU2009120812 A RU 2009120812A RU 2009120812 A RU2009120812 A RU 2009120812A
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- brucella
- suspension
- buffered
- supernatant
- separation
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1. Способ получения антигенного препарата из бруцелл в L-форме, включающий культивирование штамма Brucella aborus И-206 в L-форме на плотной питательной среде для бруцелл в L-форме при 37°C в течение 3-5 суток, смывание микробной массы забуференным 0,9% раствором хлорида натрия (pH 7,2±0,2), инактивацию добавлением 2,5%(об./об.) формалина, выдерживание бактериальной суспензии сутки при 37°C, контроль специфической стерильности, доведение бактериальной суспензии до концентрации 4,5·1010-5·1010 м.к. в мл забуференным 0,9% раствором хлорида натрия (pH 7,2±0,2), проведение термообработки суспензии на водяной бане при 100°C в течение 45-50 мин, центрифугирование суспензии при 7000 об/мин в течение 50-60 мин, отделение и лиофилизацию надосадочной жидкости. ! 2. Способ по п.1, отличающийся тем, что после отделения надосадочную жидкость разливают по 1 мл. 1. A method of obtaining an antigenic preparation from brucella in L-form, comprising cultivating a strain of Brucella aborus I-206 in L-form on a solid nutrient medium for brucella in L-form at 37 ° C for 3-5 days, washing off the microbial mass with a buffered 0.9% sodium chloride solution (pH 7.2 ± 0.2), inactivation by adding 2.5% (v / v) formalin, keeping the bacterial suspension at 37 ° C for a day, monitoring specific sterility, adjusting the bacterial suspension to concentration 4.5 · 1010-5 · 1010 m.k. in ml with a buffered 0.9% sodium chloride solution (pH 7.2 ± 0.2), heat treatment of the suspension in a water bath at 100 ° C for 45-50 min, centrifugation of the suspension at 7000 rpm for 50-60 min, separation and lyophilization of the supernatant. ! 2. The method according to claim 1, characterized in that after separation, the supernatant is poured into 1 ml.
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RU2009120812/15A RU2416429C2 (en) | 2009-06-01 | 2009-06-01 | Method for producing antigen preparation of l-brucellas |
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RU2009120812/15A RU2416429C2 (en) | 2009-06-01 | 2009-06-01 | Method for producing antigen preparation of l-brucellas |
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RU2009120812A true RU2009120812A (en) | 2010-12-10 |
RU2416429C2 RU2416429C2 (en) | 2011-04-20 |
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RU2009120812/15A RU2416429C2 (en) | 2009-06-01 | 2009-06-01 | Method for producing antigen preparation of l-brucellas |
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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RU2539827C1 (en) * | 2013-08-27 | 2015-01-27 | Государственное научное учреждение Всероссийский научно-исследовательский институт бруцеллеза и туберкулеза животных Российской академии сельскохозяйственных наук (ГНУ ВНИИБТЖ Россельхозакадемии) | Method of obtaining of brucellous l-antigen |
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RU2416429C2 (en) | 2011-04-20 |
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Effective date: 20130602 |