RU2009113656A - EXPRESS METHOD FOR BIOTESTING NATURAL, WASTE WATERS AND AQUEOUS SOLUTIONS - Google Patents
EXPRESS METHOD FOR BIOTESTING NATURAL, WASTE WATERS AND AQUEOUS SOLUTIONS Download PDFInfo
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- RU2009113656A RU2009113656A RU2009113656/10A RU2009113656A RU2009113656A RU 2009113656 A RU2009113656 A RU 2009113656A RU 2009113656/10 A RU2009113656/10 A RU 2009113656/10A RU 2009113656 A RU2009113656 A RU 2009113656A RU 2009113656 A RU2009113656 A RU 2009113656A
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- RU
- Russia
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- samples
- bioluminescent
- biotesting
- natural
- aqueous solutions
- Prior art date
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Экспресс-способ биотестирования природных, сточных вод и водных растворов, включающий приготовление рабочей и контрольной биолюминесцентных проб на основе ферментов и измерение кинетики биолюминесценции, отличающийся тем, что при приготовлении контрольной пробы используют биолюминесцентный биомодуль с добавлением дистиллированной воды или фосфатного буфера, а при приготовлении рабочей пробы - биолюминесцентный биомодуль с добавлением анализируемого образца воды, выдерживают обе пробы в течение 30-300 с, запускают реакцию биолюминесценции в обеих пробах раствором ФМН, регистрируют интенсивность свечения или время достижения максимальной интенсивности свечения биолюминесценции обеих проб и по отношению этих параметров определяют токсичность исследуемого образца воды. An express method for biotesting natural, waste water and aqueous solutions, including the preparation of working and control bioluminescent samples based on enzymes and measuring the kinetics of bioluminescence, characterized in that when preparing a control sample, a bioluminescent biomodule with the addition of distilled water or phosphate buffer is used, and in the preparation of the working samples - bioluminescent biomodule with the addition of an analyzed water sample, withstand both samples for 30-300 s, start the bioluminescent reaction In both samples, the FMN solution records the luminous intensity or the time to reach the maximum luminescence intensity of the bioluminescence of both samples, and the toxicity of the studied water sample is determined from these parameters.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009113656/10A RU2413771C2 (en) | 2009-04-10 | 2009-04-10 | Rapid method for biotesting natural and waste water and aqueous solutions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009113656/10A RU2413771C2 (en) | 2009-04-10 | 2009-04-10 | Rapid method for biotesting natural and waste water and aqueous solutions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2009113656A true RU2009113656A (en) | 2010-10-20 |
| RU2413771C2 RU2413771C2 (en) | 2011-03-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2009113656/10A RU2413771C2 (en) | 2009-04-10 | 2009-04-10 | Rapid method for biotesting natural and waste water and aqueous solutions |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2413771C2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175606A (en) * | 2011-01-19 | 2011-09-07 | 西安建筑科技大学 | Method for detecting acute biological toxicity of sewage |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2483294C2 (en) * | 2011-03-29 | 2013-05-27 | Арнольд Митрофанович Скрипкин | Method for laser-spark emission determination of toxic elements in food raw material and products |
-
2009
- 2009-04-10 RU RU2009113656/10A patent/RU2413771C2/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175606A (en) * | 2011-01-19 | 2011-09-07 | 西安建筑科技大学 | Method for detecting acute biological toxicity of sewage |
| CN102175606B (en) * | 2011-01-19 | 2012-07-11 | 西安建筑科技大学 | Method for detecting acute biological toxicity of sewage |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2413771C2 (en) | 2011-03-10 |
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| PD4A | Correction of name of patent owner | ||
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20200411 |