RU2009110636A - BIOLUMINESCENT BIOMODULE AND METHOD FOR PREPARING IT - Google Patents
BIOLUMINESCENT BIOMODULE AND METHOD FOR PREPARING IT Download PDFInfo
- Publication number
- RU2009110636A RU2009110636A RU2009110636/10A RU2009110636A RU2009110636A RU 2009110636 A RU2009110636 A RU 2009110636A RU 2009110636/10 A RU2009110636/10 A RU 2009110636/10A RU 2009110636 A RU2009110636 A RU 2009110636A RU 2009110636 A RU2009110636 A RU 2009110636A
- Authority
- RU
- Russia
- Prior art keywords
- biomodule
- nadh
- gel
- fmn
- bioluminescent
- Prior art date
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- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
1. Биолюминесцентный биомодуль, содержащий иммобилизованные в гель люциферазу, НАДН:ФМН-оксидоредуктазу, миристиновый альдегид, НАДН, раствор ФМН и подложку, отличающийся тем, что содержит стабилизатор ферментов. ! 2. Способ приготовления биолюминесцентного биомодуля, включающий приготовление геля в фосфатном буфере, охлаждение геля до 24-30°С, смешивание буферного раствора биферментной системы светящихся бактерий НАДН:ФМН-оксидоредуктаза-люцифераза, растворов миристинового альдегида, восстановленного никотинамидадениндинуклеотида с гелем, дозирование на подложку и высушивание при 4-10°С, отличающийся тем, что первоначальное высушивание компонентов биомодуля производят в присутствии стабилизатора, а активируют биомодуль раствором флавинмононуклеотида. 1. Bioluminescent biomodule containing luciferase immobilized into a gel, NADH: FMN oxidoreductase, myristic aldehyde, NADH, FMN solution and a substrate, characterized in that it contains an enzyme stabilizer. ! 2. A method of preparing a bioluminescent biomodule, including the preparation of a gel in phosphate buffer, cooling the gel to 24-30 ° C, mixing a buffer solution of the bienzyme system of luminous bacteria NADH: FMN-oxidoreductase-luciferase, solutions of myristine aldehyde, reduced nicotinamide adenide dinucleotide, with and drying at 4-10 ° C, characterized in that the initial drying of the components of the biomodule is carried out in the presence of a stabilizer, and the biomodule is activated with a solution of flavinmonone leotida.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009110636/10A RU2413772C2 (en) | 2009-03-23 | 2009-03-23 | Bioluminescent biomodule for analysing toxicity of various media and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009110636/10A RU2413772C2 (en) | 2009-03-23 | 2009-03-23 | Bioluminescent biomodule for analysing toxicity of various media and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2009110636A true RU2009110636A (en) | 2010-09-27 |
| RU2413772C2 RU2413772C2 (en) | 2011-03-10 |
Family
ID=42939978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2009110636/10A RU2413772C2 (en) | 2009-03-23 | 2009-03-23 | Bioluminescent biomodule for analysing toxicity of various media and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2413772C2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2734621C1 (en) * | 2019-10-17 | 2020-10-21 | Федеральное государственное автономное образовательное учреждение высшего образования "Сибирский федеральный университет" | Enzymatic method of assessing integral toxicity of air |
-
2009
- 2009-03-23 RU RU2009110636/10A patent/RU2413772C2/en active
Also Published As
| Publication number | Publication date |
|---|---|
| RU2413772C2 (en) | 2011-03-10 |
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Free format text: LICENCE Effective date: 20121114 |