RU2008131840A - METHOD FOR SUSPENSION CULTIVATION OF HUMAN AND ANIMAL CELLS IN DRAINLESS CIRCULAR MEDIA AND ENVIRONMENT FOR ITS IMPLEMENTATION - Google Patents
METHOD FOR SUSPENSION CULTIVATION OF HUMAN AND ANIMAL CELLS IN DRAINLESS CIRCULAR MEDIA AND ENVIRONMENT FOR ITS IMPLEMENTATION Download PDFInfo
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- RU2008131840A RU2008131840A RU2008131840/13A RU2008131840A RU2008131840A RU 2008131840 A RU2008131840 A RU 2008131840A RU 2008131840/13 A RU2008131840/13 A RU 2008131840/13A RU 2008131840 A RU2008131840 A RU 2008131840A RU 2008131840 A RU2008131840 A RU 2008131840A
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Abstract
1. Способ суспензионного культивирования клеток человека и животных в бессывороточных средах, предусматривающий инкубирование клеточного материала в жидкой питательной бессывороточной среде при +37°С, причем культивирование прикрепляемых клеток HeLa и СНО-К1 осуществляют в суспензионной культуре, отличающийся тем, что инкубирование клеток неограниченно производят в полноценной бессывороточной среде, в которой клетки переходят из прикрепленного состояния в суспензионное и происходит процесс их размножения во всем объеме среды. ! 2. Способ суспензионного культивирования клеток человека и животных в бессывороточных средах по п.1, отличающийся тем, что инкубирование клеток проводят в культуральных флаконах или планшетах, которые помещают в CO2-инкубатор в атмосфере 5% CO2 и выдерживают от 72 до 168 ч. ! 3. Бессывороточная среда для культивирования клеток человека и животных, включающая глюкозу, органические и неорганические соли, аминокислоты, отличающаяся тем, что дополнительно содержит инсулин и неионные детергенты Tween 80, Pluronic F68 для обеспечения углеводного и липидного обмена клеток в бессывороточных условиях культивирования.1. The method of suspension cultivation of human and animal cells in serum-free media, comprising incubating the cellular material in a liquid nutrient serum-free medium at + 37 ° C, and the cultivation of the attached cells HeLa and CHO-K1 is carried out in a suspension culture, characterized in that the incubation of the cells is unlimited in a full-fledged serum-free medium, in which the cells pass from the attached state to the suspension state and the process of their reproduction takes place in the entire volume of the medium. ! 2. The method of suspension cultivation of human and animal cells in serum-free media according to claim 1, characterized in that the incubation of the cells is carried out in culture bottles or tablets, which are placed in a CO2 incubator in an atmosphere of 5% CO2 and incubated from 72 to 168 hours! 3. Serum-free medium for the cultivation of human and animal cells, including glucose, organic and inorganic salts, amino acids, characterized in that it additionally contains insulin and non-ionic detergents Tween 80, Pluronic F68 to ensure carbohydrate and lipid metabolism of cells in serum-free culture conditions.
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Priority Applications (1)
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RU2008131840/13A RU2008131840A (en) | 2008-08-05 | 2008-08-05 | METHOD FOR SUSPENSION CULTIVATION OF HUMAN AND ANIMAL CELLS IN DRAINLESS CIRCULAR MEDIA AND ENVIRONMENT FOR ITS IMPLEMENTATION |
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RU2008131840/13A RU2008131840A (en) | 2008-08-05 | 2008-08-05 | METHOD FOR SUSPENSION CULTIVATION OF HUMAN AND ANIMAL CELLS IN DRAINLESS CIRCULAR MEDIA AND ENVIRONMENT FOR ITS IMPLEMENTATION |
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RU2008131840A true RU2008131840A (en) | 2010-02-10 |
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RU2008131840/13A RU2008131840A (en) | 2008-08-05 | 2008-08-05 | METHOD FOR SUSPENSION CULTIVATION OF HUMAN AND ANIMAL CELLS IN DRAINLESS CIRCULAR MEDIA AND ENVIRONMENT FOR ITS IMPLEMENTATION |
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RU (1) | RU2008131840A (en) |
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- 2008-08-05 RU RU2008131840/13A patent/RU2008131840A/en not_active Application Discontinuation
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Legal Events
Date | Code | Title | Description |
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FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20100624 |