RU2007120876A - METHOD FOR PRODUCING BiodIESEL FUEL - Google Patents
METHOD FOR PRODUCING BiodIESEL FUEL Download PDFInfo
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- RU2007120876A RU2007120876A RU2007120876/13A RU2007120876A RU2007120876A RU 2007120876 A RU2007120876 A RU 2007120876A RU 2007120876/13 A RU2007120876/13 A RU 2007120876/13A RU 2007120876 A RU2007120876 A RU 2007120876A RU 2007120876 A RU2007120876 A RU 2007120876A
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- RU
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- minutes
- biomass
- washing
- biodiesel fuel
- ethanol
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1. Способ получения биодизельного топлива, включающий культивирование мицелиальных мукоровых грибов рода Cunninghamella на питательной среде состава, мас.%: глюкоза - 5, NHNO- 0,12, дрожжевой экстракт - 0,1, КНРО- 0,1, MgSO- 0,025, в концентрации 2,5-5,1·10конидий в мл в течение 116-120 ч с последующим отделением биомассы, экстрагированием из нее липидов и направлением их на стадию щелочного метанолиза с получением биодизельного топлива, соответствующего показателям европейского стандарта EN 14214, а обезжиренной биомассы - на стадию получения хитина.2. Способ по п.1, отличающийся тем, что обезжиренную биомассу выдерживают в горячей воде с добавлением 0,02-0,05% детергента «Лотос» и 1% спирта при 98°С в течение часа, после промывки осадка добавляют этанол с 1% щелочью (10:1) и процесс ведут в течение 30 мин при постоянном перемешивании с последующим отделением и отмывкой хитинсодержащего осадка, нагреванием его в течение 30 мин с 0,5% HCl при температуре 95-98° с дальнейшей отмывкой, выдерживанием с 1% NaOH при температуре 95-98° в течение 25-30 мин, обработкой этанолом и лиофилизацией или высушиванием.1. A method of producing biodiesel fuel, including the cultivation of mycelial mucoric fungi of the genus Cunninghamella on a nutrient medium composition, wt.%: Glucose - 5, NHNO-0.12, yeast extract - 0.1, KNRO-0.1, MgSO-0,025, at a concentration of 2.5-5.1 · 10conidia in ml for 116-120 hours, followed by separation of the biomass, extraction of lipids from it and their direction to the alkaline methanolysis stage to obtain biodiesel fuel corresponding to the European standard EN 14214, and fat-free biomass - to the stage of obtaining chitin. 2. The method according to claim 1, characterized in that the non-fat biomass is kept in hot water with the addition of 0.02-0.05% Lotus detergent and 1% alcohol at 98 ° C for an hour, after washing the precipitate, ethanol with 1% is added with alkali (10: 1) and the process is carried out for 30 minutes with constant stirring, followed by separation and washing of the chitin-containing precipitate, heating it for 30 minutes with 0.5% HCl at a temperature of 95-98 ° with further washing, maintaining with 1% NaOH at a temperature of 95-98 ° for 25-30 minutes, treatment with ethanol and lyophilization or drying.
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2007120876/13A RU2007120876A (en) | 2007-06-05 | 2007-06-05 | METHOD FOR PRODUCING BiodIESEL FUEL |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2007120876/13A RU2007120876A (en) | 2007-06-05 | 2007-06-05 | METHOD FOR PRODUCING BiodIESEL FUEL |
Publications (1)
Publication Number | Publication Date |
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RU2007120876A true RU2007120876A (en) | 2008-12-10 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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RU2007120876/13A RU2007120876A (en) | 2007-06-05 | 2007-06-05 | METHOD FOR PRODUCING BiodIESEL FUEL |
Country Status (1)
Country | Link |
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RU (1) | RU2007120876A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2667363C1 (en) * | 2017-05-22 | 2018-09-19 | Алексей Сергеевич Архипов | Method of obtaining motor fuel (biodiesel) |
-
2007
- 2007-06-05 RU RU2007120876/13A patent/RU2007120876A/en not_active Application Discontinuation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2667363C1 (en) * | 2017-05-22 | 2018-09-19 | Алексей Сергеевич Архипов | Method of obtaining motor fuel (biodiesel) |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20090211 |