RU2004246C1 - Contrast-medicinal medium for us-therapy of inflammatory processes - Google Patents

Abstract

The invention relates to medicine and can be used for anti-inflammatory ultrasound therapy. The purpose of the invention is to increase specific activity and reduce adverse reactions. The essence of the invention is in the preparation of a preparation containing a lipid extract from natural silt loams of 0.5-1.5 wt.% And vegetable oil up to 100. The positive effect consists in enhancing the penetration of a biologically active substance into the skin and in the absence of adverse reactions 1 table

Description

The invention relates to medicine and can be used for anti-inflammatory ultrasound (US) therapy.

Various drug contact media are known consisting of anti-inflammatory synthetic formulations and a vaseline or vaseline-lanolin base. However, these media, including a viscous base (petroleum jelly), have insufficient penetration of drugs into the skin.

The closest analogue to the claimed medium is a contact medicinal medium for anti-inflammatory ultrasound therapy containing, as a basis, petrolatum with the addition of a synthetic hormonal drug, hydrocortisone.

However, the content of synthetic medicinal substances makes it possible to produce negative and adverse reactions up to an exacerbation of the inflammatory process, and the inclusion of a vaseline base in the medium makes it difficult for the drug to penetrate the skin.

An object of the invention is to increase specific activity and reduce adverse reactions.

The goal is achieved in that the contact medicinal medium includes the following ingredients, wt.%.

Lipid extract

from natural silt

gr zey 0.5-1.5

Vegetable oil is 98.5-99.5, while the lipid extract from natural sludge therapeutic mud is a biologically active substance (BAS) with a pronounced anti-inflammatory effect.

Of the main classes of compounds, the lipid extract contains,%: saturated and unsaturated hydrocarbons 9.2; ketoalcohols 6; sterols and their esters 12.4; cerebrosides 7.3; higher amino alcohols 9.0, glycerides 10.6; sulfolipids 10.7; acid glycerophospharides 8.6; phospholipids 5.3.

The study of the permeability of the lipid extract through intact skin was carried out by the isotopic method with poiod-131 labeled oleic acid on 35 white mongrel male rats weighing 180-200 g in 8 series of experiments:

1. Skin application of the drug in vegetable oil (0.5%, 1%, 1.5%).

2. The same, but with ultrasonic action (0.5% -1.5%).

3. Skin application of the drug in a lanolin-vaseline basis.

4. The same, but with ultrasonic exposure.

5. Topically applied 0.5 g of vegetable oil.

6. The same, but with ultrasonic exposure. .

7. Skin application of 0.5 g of petrolatum-vaseline.

8. The same, but with ultrasonic exposure.

A technique for preparing a contact drug mixed with an isotope to study its permeability to the skin. Lipidna

the extract in the form of a 0.5-1.5% solution in vaseline-lanolin and vegetable oil with the addition of 0.1 ml of the isotope of oleic acid Is1 (activity 1 μK / m, l) was thoroughly mixed and applied to the clipped

the surface of the back skin with an area of 25-30 cm in an amount of 0.5 g. The time of application was 2 hours, ultrasound was applied by the LOR-3 apparatus with an intensity of 0.6 W / cm2 at a frequency of 880 kHz for 10 min according to the labile

technique in constant mode. After the procedure, the contact mixture was carefully removed from the skin with a gauze swab moistened with alcohol. After animal decantation, the activity of skin samples with an area of 1 cm2 was measured on

well counter NZ-350 from the installation Gamma (Hungary). The percentage of 1131 skin content of the total activity deposited on the skin surface in each experiment was calculated, as well as the percentage of deposition .1

| $ 31 per 1 cm2 of rat skin surface of the total applied activity.

PRI me R 1. Lipid extract

from natural silt mud 0.5L. 100 g of medium dark brown.

Cooking Oil99.5 /

Grind thoroughly and mix for 15 minutes.

PRI me R 2. Lipid extract

from natural sludge mud, 1.0 T. 100 g of medium of dark brown color.

Cooking Oil99.0 /

Grind thoroughly and mix for 15 minutes.

PRI me R 3. Lipid extract

from natural sludge mud 1. 100 g medium dark brown

Cooking Oil98.5)

Grind thoroughly and mix for 15 minutes.

The results of the studies showed that the accumulation of lipid extracts in the skin significantly depends on the type of those substances that are used as a base, as well as on the ultrasound skin treatment.

The table shows that under the action of ultrasound (control 1) 3 times more isotope is accumulated in the skin from vegetable oil than from the petrolatum-lanolin mixture (control-2). When a lipid extract is added to vegetable oil and ultrasound is applied, 2.5 times more oleic acid isotope is accumulated in the skin than from a solution of lipids in petrolatum-lanolin base. The best result of oleic acid penetration into the skin was noted from a solution of lipid extract at a concentration of 1%.

Thus, the addition of a lipid pulp extract to a vegetable oil of 0.5-1.5% gives a much greater accumulation of the isotope in the skin than from a petrolatum-lanolin base in the same concentration.

Based on the experiments, it can be argued that, under the action of ultrasound, oleic acid and, apparently, similar in structure and properties to the fatty acids contained in the lipid extract of the mud, enter the skin from a solution of lipids in vegetable oil. Moreover, from a solution of lipids to grow, an assessment of the permeability of the skin of rats after the applied contact drug among for ultrasound therapy

In oily oil, a significantly larger amount of oleic acid penetrates into the skin than from a lipid solution in a vaseline-lanolin base.

Obviously these results are necessary

to consider when developing composite dosage forms prepared for anti-inflammatory treatment.

Advantages of the claimed contact drug medium in comparison with the known contact drug media of the same purpose, including the base object (prototype), are: -.

in the use of a natural biologically active substance with an anti-inflammatory effect, and therefore, when used for therapeutic purposes, there are no negative side effects

events and exacerbations;

in enhancing the penetration into the skin of a biologically active substance and its accumulation by using a base of vegetable oils (see table);

in reducing the cost of treatment for the claimed environment by 3.1 times compared with the prototype.

(56) Questions of balneology. 1977, 1, pp. 29-33.