RU2004107863A - METHOD FOR PRESERVATION OF AMNIOTIC MEMBRANE - Google Patents

METHOD FOR PRESERVATION OF AMNIOTIC MEMBRANE Download PDF

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Publication number
RU2004107863A
RU2004107863A RU2004107863/15A RU2004107863A RU2004107863A RU 2004107863 A RU2004107863 A RU 2004107863A RU 2004107863/15 A RU2004107863/15 A RU 2004107863/15A RU 2004107863 A RU2004107863 A RU 2004107863A RU 2004107863 A RU2004107863 A RU 2004107863A
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RU
Russia
Prior art keywords
amniotic membrane
preservation
amniotic
lyophilized
petri dishes
Prior art date
Application number
RU2004107863/15A
Other languages
Russian (ru)
Other versions
RU2267922C2 (en
Inventor
Елена Анатольевна Федорова (RU)
Елена Анатольевна Федорова
Алексей Юрьевич Слонимский (RU)
Алексей Юрьевич Слонимский
Юрий Евгеньевич Батманов (RU)
Юрий Евгеньевич Батманов
Original Assignee
Российский государственный медицинский университет (RU)
Российский государственный медицинский университет
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Российский государственный медицинский университет (RU), Российский государственный медицинский университет filed Critical Российский государственный медицинский университет (RU)
Priority to RU2004107863/15A priority Critical patent/RU2267922C2/en
Publication of RU2004107863A publication Critical patent/RU2004107863A/en
Application granted granted Critical
Publication of RU2267922C2 publication Critical patent/RU2267922C2/en

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Claims (1)

Способ консервации амниотической мембраны, включающий забор биологического материала, очистку амниотической оболочки от сгустков крови и остатков хориона, отличающийся тем, что, с целью повышения эффективности и увеличения сроков хранения консервированной амниотической мембраны, предварительно нарезанные трепаном сегменты амниотической мембраны, диаметром 28-30 мм, помещают в стерильные чашки Петри, замораживают при t -40°C, лиофилизируют до остаточной влажности 2-5% в течение 24 ч, затем чашки Петри с лиофилизированной амниотической мембраной герметично упаковывают в стерилизационные пакеты, маркируют и стерилизуют γ - облучением в дозе 25 КГр.A method of preservation of the amniotic membrane, including the collection of biological material, purification of the amniotic membrane from blood clots and chorion residues, characterized in that, in order to increase efficiency and increase the shelf life of the canned amniotic membrane, segments of the amniotic membrane, previously cut by trepan, are 28-30 mm in diameter, placed in sterile Petri dishes, frozen at t -40 ° C, lyophilized to a residual moisture content of 2-5% for 24 hours, then Petri dishes with lyophilized amniotic membranes hydrochloric hermetically packaged in sterilization pouches, and sterilized marked γ - irradiation at 25 kGy.
RU2004107863/15A 2004-03-17 2004-03-17 Method for preserving amnion RU2267922C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2004107863/15A RU2267922C2 (en) 2004-03-17 2004-03-17 Method for preserving amnion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2004107863/15A RU2267922C2 (en) 2004-03-17 2004-03-17 Method for preserving amnion

Publications (2)

Publication Number Publication Date
RU2004107863A true RU2004107863A (en) 2005-09-10
RU2267922C2 RU2267922C2 (en) 2006-01-20

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ID=35847601

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2004107863/15A RU2267922C2 (en) 2004-03-17 2004-03-17 Method for preserving amnion

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RU (1) RU2267922C2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2519357C2 (en) * 2012-04-26 2014-06-10 Государственное бюджетное образовательное учреждение высшего профессионального образования "Амурская государственная медицинская академия" Минздравсоцразвития Российской Федерации Method for amniotic membrane dissection for intrastromal implantation into corneal stroma on ice-cold surface
CN107296041B (en) * 2017-07-02 2020-12-25 江西瑞济生物工程技术股份有限公司 Fresh amnion preservation solution, fresh amnion preservation method and application

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Publication number Publication date
RU2267922C2 (en) 2006-01-20

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20060318