RU2002118807A - Method for producing chlamydial antigen for indirect immunofluorescence reaction - Google Patents

Method for producing chlamydial antigen for indirect immunofluorescence reaction

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Publication number
RU2002118807A
RU2002118807A RU2002118807/15A RU2002118807A RU2002118807A RU 2002118807 A RU2002118807 A RU 2002118807A RU 2002118807/15 A RU2002118807/15 A RU 2002118807/15A RU 2002118807 A RU2002118807 A RU 2002118807A RU 2002118807 A RU2002118807 A RU 2002118807A
Authority
RU
Russia
Prior art keywords
indirect immunofluorescence
corpuscles
chlamydial antigen
producing
immunofluorescence reaction
Prior art date
Application number
RU2002118807/15A
Other languages
Russian (ru)
Other versions
RU2223497C1 (en
Inventor
Ольга Анатольевна Тимашева
Светлана Александровна Басалгина
Original Assignee
Федеральное государственное унитарное предпри тие "Научно-производственное объединение по медицинским иммунологическим препаратам "Микроген" Министерства здравоохранени Российской Федерации
Федеральное государственное унитарное предприятие "Научно-производственное объединение по медицинским иммунологическим препаратам "МИКРОГЕН" Министерства здравоохранения Российской Федерации
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Федеральное государственное унитарное предпри тие "Научно-производственное объединение по медицинским иммунологическим препаратам "Микроген" Министерства здравоохранени Российской Федерации, Федеральное государственное унитарное предприятие "Научно-производственное объединение по медицинским иммунологическим препаратам "МИКРОГЕН" Министерства здравоохранения Российской Федерации filed Critical Федеральное государственное унитарное предпри тие "Научно-производственное объединение по медицинским иммунологическим препаратам "Микроген" Министерства здравоохранени Российской Федерации
Priority to RU2002118807/15A priority Critical patent/RU2223497C1/en
Application granted granted Critical
Publication of RU2223497C1 publication Critical patent/RU2223497C1/en
Publication of RU2002118807A publication Critical patent/RU2002118807A/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicinal Preparation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Claims (1)

Способ получения хламидийного антигена для реакции непрямой иммунофлуоресценции, включающий культивирование хламидий в куриных эмбрионах, гомогенизацию инфицированных желточных мешков, суспендирование корпускул в среде 199 с последующей инактивацией формалином в смеси с азидом натрия в конечной концентрации 0,05 и 0,1% на объем суспензии соответственно, отделением целевого продукта центрифугированием, смешиванием со стабилизатором состава, мас.%: среда 199-98-99,5; сахароза - 0,5-2 и лиофилизацией, отличающийся тем, что после инактивации проводят дифференциальное центрифугирование при 150 и 500 g в течение 10 мин при температуре (3±1)°С, средний слой (целевой продукт) фильтруют через мембрану с размерами пор 3 мкм под давлением (0,3±0,1) МПа при температуре (13±3)°С, концентрируют, фильтрат стандартизуют, доводя средой 199 до содержания 100 корпускул в поле зрения препарата.A method of producing a chlamydial antigen for an indirect immunofluorescence reaction, including culturing chlamydia in chicken embryos, homogenizing infected yolk sacs, suspending the corpuscles in 199 medium, followed by formalin inactivation in a mixture with sodium azide in a final concentration of 0.05 and 0.1% per suspension volume, respectively , separation of the target product by centrifugation, mixing with a stabilizer composition, wt.%: environment 199-98-99,5; sucrose - 0.5-2 and lyophilization, characterized in that after inactivation, differential centrifugation is carried out at 150 and 500 g for 10 min at a temperature of (3 ± 1) ° С, the middle layer (target product) is filtered through a membrane with pore sizes 3 μm under pressure (0.3 ± 0.1) MPa at a temperature of (13 ± 3) ° C, concentrated, the filtrate was standardized, bringing medium 199 to the content of 100 corpuscles in the field of view of the drug.
RU2002118807/15A 2002-07-12 2002-07-12 Method for obtaining chlamydial antigen for indirect immunofluorescence reaction RU2223497C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2002118807/15A RU2223497C1 (en) 2002-07-12 2002-07-12 Method for obtaining chlamydial antigen for indirect immunofluorescence reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2002118807/15A RU2223497C1 (en) 2002-07-12 2002-07-12 Method for obtaining chlamydial antigen for indirect immunofluorescence reaction

Publications (2)

Publication Number Publication Date
RU2223497C1 RU2223497C1 (en) 2004-02-10
RU2002118807A true RU2002118807A (en) 2004-02-27

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RU2002118807/15A RU2223497C1 (en) 2002-07-12 2002-07-12 Method for obtaining chlamydial antigen for indirect immunofluorescence reaction

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RU2223497C1 (en) 2004-02-10

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Effective date: 20070713