RU2002106236A - IDENTIFICATION AND IDENTIFICATION OF BACTERIAL STRAINS - Google Patents

IDENTIFICATION AND IDENTIFICATION OF BACTERIAL STRAINS

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Publication number
RU2002106236A
RU2002106236A RU2002106236/13A RU2002106236A RU2002106236A RU 2002106236 A RU2002106236 A RU 2002106236A RU 2002106236/13 A RU2002106236/13 A RU 2002106236/13A RU 2002106236 A RU2002106236 A RU 2002106236A RU 2002106236 A RU2002106236 A RU 2002106236A
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RU
Russia
Prior art keywords
bacteriophages
carrier
bacteria
proteins
detecting
Prior art date
Application number
RU2002106236/13A
Other languages
Russian (ru)
Other versions
RU2265061C2 (en
Inventor
Штефан МИЛЛЕР
Original Assignee
Профос АГ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Профос АГ filed Critical Профос АГ
Publication of RU2002106236A publication Critical patent/RU2002106236A/en
Application granted granted Critical
Publication of RU2265061C2 publication Critical patent/RU2265061C2/en

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Claims (10)

1. Способ выявления бактерий, при котором осуществляют следующие стадии: а) соединяют бактериофаги и/или белки бактериофагов с носителем либо путем соединения бактериофагов и/или белков бактериофагов с носителем непосредственным связыванием, либо путем непосредственного связывания бактериофагов и/или белков бактериофагов с полипептидом, иммобилизованным на носителе, б) носитель, соединенный с бактериофагами и/или белками бактериофагов, инкубируют с образцом, в) возможно удаляют образец и бактерии в образце, не связавшиеся с бактериофагами и/или белками бактериофагов, г) возможно добавляют вещества, пермеабилизирущие или разрушающие мембрану бактерий, и д) выявляют в образце бактерии, связавшиеся с бактериофагами и/или белками бактериофагов, причем указанные связавшиеся бактерии не подвергают стадии культивирования.1. A method for detecting bacteria in which the following steps are carried out: a) bacteriophages and / or bacteriophage proteins are connected to a carrier either by directly binding bacteriophages and / or bacteriophage proteins to a carrier, or by directly binding bacteriophages and / or bacteriophage proteins to a polypeptide, immobilized on a carrier, b) a carrier connected to bacteriophages and / or proteins of bacteriophages is incubated with the sample, c) it is possible to remove the sample and bacteria in the sample that did not contact the bacterioph s and / or bacteriophage proteins, d) optionally added substances permeabiliziruschie or membrane disrupting the bacteria and d) detecting in the sample is bacteria bound to the bacteriophages and / or bacteriophage proteins, wherein said unbound bacteria are not subjected to the culturing step. 2. Способ по п.1, при котором выявление осуществляют путем колориметрического выявления клеточных компонентов и/или продуктов размножения фагов, путем выявления ДНК и/или РНК или с помощью иммуноанализа.2. The method according to claim 1, wherein the detection is carried out by colorimetric detection of cellular components and / or phage propagation products, by detecting DNA and / or RNA, or by immunoassay. 3. Способ по п.1 или 2, при котором бактериофаги и/или белки бактериофагов, непосредственно связываемые с носителем, соединяют с этим носителем посредством адсорбции или посредством химического связывания.3. The method according to claim 1 or 2, in which bacteriophages and / or bacteriophage proteins that are directly associated with the carrier, are connected to this carrier by adsorption or by chemical binding. 4. Способ по п.1 или 2, где полипептиды, иммобилизованные на носителе, представляют собой специфичные антитела, лектины, рецепторы или антикалины.4. The method according to claim 1 or 2, where the polypeptides immobilized on a carrier are specific antibodies, lectins, receptors or anticalins. 5. Способ по любому из пп.1-4, где бактериофаги и/или белки бактериофагов демонстрируют модификации.5. The method according to any one of claims 1 to 4, where bacteriophages and / or proteins of bacteriophages show modifications. 6. Способ по любому из пп.1-5, при котором используют по меньшей мере два разных бактериофага и/или белка бактериофагов, распознающих по меньшей мере два разных типа и/или рода бактерий.6. The method according to any one of claims 1 to 5, in which at least two different bacteriophages and / or bacteriophage proteins are used that recognize at least two different types and / or types of bacteria. 7. Способ по любому из пп.1-6, где носитель представляет собой титрационный микропланшет, тестовую полоску, предметное стекло, пластинку, фильтрующий материал или проточную клеточную камеру из полистирола, полипропилена, поликарбоната, полиметилметакрилата (ПММА), ацетата целлюлозы, нитроцеллюлозы, стекла или кремниевой пластинки.7. The method according to any one of claims 1 to 6, where the carrier is a microtiter plate, a test strip, a glass slide, a plate, a filter material or a flow cell chamber made of polystyrene, polypropylene, polycarbonate, polymethyl methacrylate (PMMA), cellulose acetate, nitrocellulose, glass or silicon wafer. 8. Применение способа по любому из пп.1-7 для выявления бактерий в области медицины, пищевой промышленности и в анализе, в области животноводства, анализа пресной воды или окружающей среды.8. The use of the method according to any one of claims 1 to 7 for detecting bacteria in the field of medicine, food industry and in the analysis, in the field of animal husbandry, analysis of fresh water or the environment. 9. Набор для осуществления способа по любому из пп.1-7, содержащий носитель с иммобилизованными фагами или белками фагов и реактивы для анализа для выявления связавшихся бактерий.9. A kit for implementing the method according to any one of claims 1 to 7, containing a carrier with immobilized phages or phage proteins and assay reagents for detecting bound bacteria. 10. Набор по п.9, дополнительно содержащий растворы для отмывки и/или вещества, пермеабилизирующие или разрушающие мембрану бактерий.10. The kit according to claim 9, additionally containing solutions for washing and / or substances permeabilizing or destroying the bacterial membrane.
RU2002106236/13A 1999-07-30 2000-07-28 Method for detection of microorganisms and set for its realization RU2265061C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19936047 1999-07-30
DE19936047.2 1999-07-30

Publications (2)

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RU2002106236A true RU2002106236A (en) 2004-09-10
RU2265061C2 RU2265061C2 (en) 2005-11-27

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Country Status (12)

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US (2) US7087376B2 (en)
EP (1) EP1198713B1 (en)
JP (1) JP5339658B2 (en)
AT (1) ATE316655T1 (en)
AU (1) AU781090B2 (en)
CA (1) CA2380480C (en)
DE (2) DE50012134D1 (en)
DK (1) DK1198713T3 (en)
ES (1) ES2256028T3 (en)
PT (1) PT1198713E (en)
RU (1) RU2265061C2 (en)
WO (1) WO2001009370A2 (en)

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