RU2001105C1 - Method for obtaining bioautogramm - Google Patents

Method for obtaining bioautogramm

Info

Publication number
RU2001105C1
RU2001105C1 SU4311937A RU2001105C1 RU 2001105 C1 RU2001105 C1 RU 2001105C1 SU 4311937 A SU4311937 A SU 4311937A RU 2001105 C1 RU2001105 C1 RU 2001105C1
Authority
RU
Russia
Prior art keywords
days
strips
chromatographic plate
test object
plate
Prior art date
Application number
Other languages
Russian (ru)
Inventor
Светлана Ивановна Николаева
Ирина Алексеевна Завелишко
Мари Васильевна Харбур
Original Assignee
Институт биологической защиты растений АН Республики Молдова
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Институт биологической защиты растений АН Республики Молдова filed Critical Институт биологической защиты растений АН Республики Молдова
Priority to SU4311937 priority Critical patent/RU2001105C1/en
Application granted granted Critical
Publication of RU2001105C1 publication Critical patent/RU2001105C1/en

Links

Description

Блинов Н.О. и Хохлов А.С. Бумажна  хроматографи  антибиотиков. М.: Наука, 1970, 364с. Сравнительна  оценка способов получени  биоавтограмм по прототипу и предлагаемомуBlinov N.O. and Khokhlov A.S. Paper chromatography of antibiotics. M .: Nauka, 1970, 364s. A comparative assessment of the methods for producing bioautograms of the prototype and the proposed

способу на примере тест-объекта S.sclerotiorum.the method on the example of the test object S.sclerotiorum.

ПрототипPrototype

Получение мицели 3 - 4 сутGetting a mycelium 3-4 days

Образование склероциев3 - 4 сутSclerotia 3 - 4 days

Промораживание склероциев30 сутFreezing sclerotia 30 days

Помещение склероциев во влажный субстрат и проращивание до обраховани  апотециев с сумкоспорами30 - 38сутPlacement of sclerotia in a moist substrate and germination until apothecia with sacospores is formed 30-30 days

Подготовка хроматографической пластины: нанесение экстракта, элюирование, высушивание 0.5 сутPreparation of a chromatographic plate: application of the extract, elution, drying 0.5 days

Внесение сумкоспор в питательную среду и покрытие ею пластины 0,5 сутThe introduction of bagospores into the nutrient medium and coating the plate with it for 0.5 days

Про вление биовтограммы2 сутBiovtogram development 2 days

Итого: 7 операций.70 сутTotal: 7 operations. 70 days

Claims (2)

1. СПОСОБ ПОЛУЧЕНИЯ БИОАВТОГРАММ. ВКЛЮчающий покрытие хроматографической пластины после разделени  на ней исследуемого раствора, содержащего антибиотик , слоем питательной среды с тест-объектом, отличающийс  тем, что, с целью расширени  круга примен емых тест-объектов, хроматографическую пластину сначала покрывают слоем стерильной питательной среды, а затем сверху,1. METHOD OF OBTAINING BIOAUTOGRAMS. Including coating the chromatographic plate after separating the test solution containing the antibiotic on it with a layer of culture medium with a test object, characterized in that, in order to expand the range of test objects used, the chromatographic plate is first covered with a layer of sterile culture medium, and then on top. Предлагаемый способThe proposed method Получение мицели 3-4 сутGetting mycelium 3-4 days не надо не надоno, no, no need не надоdo not Подготовка хроматографической пластины: нанесение экстракта, элюирование, высушивание .. сутPreparation of a chromatographic plate: application of the extract, elution, drying .. days Покрытие пластины стерильной питательной средой и наложение сверху полосок расту1Covering the plate with a sterile growth medium and overlaying strips on top of it щего мицели 0,5 сутmycelium 0.5 days Про вление биоавтограммы2 сутBioautogram development 2 days 4 операции. 7 сут4 operations. 7 days 5 вдоль боковых сторон накладывают полоски твердой питательной среды с расту- щим на ней газоном тест-объекта.5 along the lateral sides, apply strips of solid nutrient medium with the lawn of the test object growing on it. 2. Способ по п.1, отличающийс  тем, Ю что полоски твердой питательной среды с растущим «а ней газоном тест-объекта имеют ширину 3 - 4 мм, толщину 2 - 3 мм и длину, равную длине хроматографической пластины. 152. The method according to claim 1, characterized in that the strips of a solid nutrient medium with a growing lawn of the test object have a width of 3-4 mm, a thickness of 2-3 mm and a length equal to the length of the chromatographic plate. fifteen t.fx.t.fx. nn ЛL
SU4311937 1987-10-01 1987-10-01 Method for obtaining bioautogramm RU2001105C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU4311937 RU2001105C1 (en) 1987-10-01 1987-10-01 Method for obtaining bioautogramm

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU4311937 RU2001105C1 (en) 1987-10-01 1987-10-01 Method for obtaining bioautogramm

Publications (1)

Publication Number Publication Date
RU2001105C1 true RU2001105C1 (en) 1993-10-15

Family

ID=21329995

Family Applications (1)

Application Number Title Priority Date Filing Date
SU4311937 RU2001105C1 (en) 1987-10-01 1987-10-01 Method for obtaining bioautogramm

Country Status (1)

Country Link
RU (1) RU2001105C1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153400A (en) * 1999-03-12 2000-11-28 Akzo Nobel N.V. Device and method for microbial antibiotic susceptibility testing
US6251624B1 (en) 1999-03-12 2001-06-26 Akzo Nobel N.V. Apparatus and method for detecting, quantifying and characterizing microorganisms

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Егоров Н.С. Основы учени об антибиотиках. М.; Высша школа, 1986, 448 с. *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153400A (en) * 1999-03-12 2000-11-28 Akzo Nobel N.V. Device and method for microbial antibiotic susceptibility testing
US6251624B1 (en) 1999-03-12 2001-06-26 Akzo Nobel N.V. Apparatus and method for detecting, quantifying and characterizing microorganisms
US6416969B2 (en) 1999-03-12 2002-07-09 Akzo Nobel N.V. Susceptibility plates for microbial antibiotic susceptibility testing

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