RU2001105C1 - Method for obtaining bioautogramm - Google Patents
Method for obtaining bioautogrammInfo
- Publication number
- RU2001105C1 RU2001105C1 SU4311937A RU2001105C1 RU 2001105 C1 RU2001105 C1 RU 2001105C1 SU 4311937 A SU4311937 A SU 4311937A RU 2001105 C1 RU2001105 C1 RU 2001105C1
- Authority
- RU
- Russia
- Prior art keywords
- days
- strips
- chromatographic plate
- test object
- plate
- Prior art date
Links
Description
Блинов Н.О. и Хохлов А.С. Бумажна хроматографи антибиотиков. М.: Наука, 1970, 364с. Сравнительна оценка способов получени биоавтограмм по прототипу и предлагаемомуBlinov N.O. and Khokhlov A.S. Paper chromatography of antibiotics. M .: Nauka, 1970, 364s. A comparative assessment of the methods for producing bioautograms of the prototype and the proposed
способу на примере тест-объекта S.sclerotiorum.the method on the example of the test object S.sclerotiorum.
ПрототипPrototype
Получение мицели 3 - 4 сутGetting a mycelium 3-4 days
Образование склероциев3 - 4 сутSclerotia 3 - 4 days
Промораживание склероциев30 сутFreezing sclerotia 30 days
Помещение склероциев во влажный субстрат и проращивание до обраховани апотециев с сумкоспорами30 - 38сутPlacement of sclerotia in a moist substrate and germination until apothecia with sacospores is formed 30-30 days
Подготовка хроматографической пластины: нанесение экстракта, элюирование, высушивание 0.5 сутPreparation of a chromatographic plate: application of the extract, elution, drying 0.5 days
Внесение сумкоспор в питательную среду и покрытие ею пластины 0,5 сутThe introduction of bagospores into the nutrient medium and coating the plate with it for 0.5 days
Про вление биовтограммы2 сутBiovtogram development 2 days
Итого: 7 операций.70 сутTotal: 7 operations. 70 days
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU4311937 RU2001105C1 (en) | 1987-10-01 | 1987-10-01 | Method for obtaining bioautogramm |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU4311937 RU2001105C1 (en) | 1987-10-01 | 1987-10-01 | Method for obtaining bioautogramm |
Publications (1)
Publication Number | Publication Date |
---|---|
RU2001105C1 true RU2001105C1 (en) | 1993-10-15 |
Family
ID=21329995
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU4311937 RU2001105C1 (en) | 1987-10-01 | 1987-10-01 | Method for obtaining bioautogramm |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2001105C1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6153400A (en) * | 1999-03-12 | 2000-11-28 | Akzo Nobel N.V. | Device and method for microbial antibiotic susceptibility testing |
US6251624B1 (en) | 1999-03-12 | 2001-06-26 | Akzo Nobel N.V. | Apparatus and method for detecting, quantifying and characterizing microorganisms |
-
1987
- 1987-10-01 RU SU4311937 patent/RU2001105C1/en active
Non-Patent Citations (1)
Title |
---|
Егоров Н.С. Основы учени об антибиотиках. М.; Высша школа, 1986, 448 с. * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6153400A (en) * | 1999-03-12 | 2000-11-28 | Akzo Nobel N.V. | Device and method for microbial antibiotic susceptibility testing |
US6251624B1 (en) | 1999-03-12 | 2001-06-26 | Akzo Nobel N.V. | Apparatus and method for detecting, quantifying and characterizing microorganisms |
US6416969B2 (en) | 1999-03-12 | 2002-07-09 | Akzo Nobel N.V. | Susceptibility plates for microbial antibiotic susceptibility testing |
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