RU2000133243A - Nutrient medium for the culture of animal cells (options) and a method for producing protein using medium - Google Patents

Claims (13)

1. The culture medium for the culture of animal cells, characterized in that it contains the product of the enzymatic decomposition of fish meat or extract from fish meat. 2. The culture medium for the culture of an animal cell according to claim 1, which is a culture medium for producing the desired protein by culturing an animal cell, the culture medium containing an extract of fish meat. 3. The culture medium for the culture of an animal cell according to claim 1, which is a culture medium for producing the desired protein by culturing an animal cell, the culture medium containing the product of the enzymatic decomposition of fish meat. 4. The culture medium for the culture of animal cells according to claim 3, in which the product of the enzymatic decomposition of fish meat is obtained by processing fish meat or an extract from fish meat with a protease. 5. The culture medium for the culture of an animal cell according to claim 4, in which the protease is a proteinase and / or peptidase. 6. The culture medium for the culture of an animal cell according to claim 4, in which the product of the enzymatic decomposition of fish meat is obtained by treating fish meat or fish meat extract with proteinase and then peptidase. 7. The culture medium for the culture of an animal cell according to any one of claims 1 to 6, which is a medium that does not contain components derived from mammals. 8. The culture medium for the culture of an animal cell according to any one of claims 1 to 7, which contains amino acids, vitamins, lipid factors, energy sources, osmoregulators, iron sources and pH regulators. 9. The culture medium for the culture of an animal cell according to any one of claims 1 to 8, in which the animal cell is an animal cell into which a gene encoding the desired protein is introduced by transduction. 10. The culture medium for the culture of an animal cell according to claim 9, in which the desired protein is an antibody or physiologically active substance. 11. The culture medium for the culture of an animal cell according to any one of claims 1 to 10, in which the animal cell is a CHO cell. 12. The culture medium for the culture of an animal cell according to any one of claims 1 to 11, in which fish meat is obtained from one or more species of fish selected from the group consisting of bonito, mackerel tuna, cod, mackerel, salmon and sardine. 13. A method of obtaining the desired protein of animal cell cultivation pathways in which cultivation is carried out using a culture medium for animal cell culture, wherein the culture medium is characterized in that it contains an enzymatic decomposition product of fish meat or an extract from fish meat.