RU1825629C - Method for prophylaxis of tuberculosis at calves - Google Patents

Abstract

Usage: in veterinary medicine, in particular for the treatment of animals. SUMMARY OF THE INVENTION: Isoniazid is used for treatment.

Description

The invention relates to veterinary medicine, in particular to the prevention of tuberculosis of young cattle

The aim of the invention is to accelerate the absorption of the drug and increase its prophylactic activity.

The essence of the method for the prevention of tuberculosis of the body is in the subcutaneous administration of the bodies of a preparation containing isoniazid and a base, and an etericide is used as a base in the following ratio of components:

Isoniazid 100-200 g

Etericide 1 L

moreover, the drug is administered subcutaneously at a dose of 10 mg / kg body weight according to the content of isoniazid with an interval of 8 to 12 days for 3 months.

With this ratio of components, isoniazid dissolves well. At doses of more than 200 g, isoniazid does not completely dissolve and crystallizes even when heated to 37 ° C.

The proposed drug has an inherent in other isoniazid drugs property prolonged action:

when it is used, the prophylactic effect is provided for 10-12 days. The action of isoniazid preparations on water or fish oil (tubazide mixture), used orally or subcutaneously, is limited to 2-3 days.

Isoniazid, which is part of the preparation, has high bacteriostatic properties on tuberculosis mycobacteria, but with prolonged use it reduces the protein balance in tissues.

Etericide is a derivative of the oxidation of water-soluble bactericidal fractions of fish oil (3-11). The drug is altogether represented by aldehydes, unsaturated fatty acids and peroxides, a clear liquid with a slight specific odor, density 1.005-1.007 g / cm3, yellowish, pH 2.8-3.2, has a pronounced antibacterial activity against pyogenic microflora (Proteus, staphylococcus aureus, blue-green pus and intestinal bacillus, including antibiotic-resistant, acts on mycobacterium tuberculosis, reducing their virulence Regardless of the method of administration (intravenously

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intraperitoneally, parenterally, orally and topically) under conditions of repeated administration (15-20 days), the etericide does not cause allergic and toxic reactions in laboratory animals.5

The isoniazid preparation on an etericide is a liquid of a dark straw color, its 10% solution at a temperature of + 4 to + 20 ° C and no longer crystallizes. In the same temperature range, a 20% solution of Yu isoniazid on an etericide crystallizes in 60 minutes and re-dissolves by heating to 37 ° C. The drug is made as follows: 15

isoniazid and ekteritsid take in the following ratio:

Isoniazid (tubazide) 100-200 g

Etericiddo 1 L

For example, 100-200 g of isoniazid is poured into a volumetric flask or a volumetric 20 beaker and up to 1 liter of an extericide preheated to 45 ° C is added. After dissolving isoniazid, the preparation can be used immediately for administration to animals.

To store the preparation, it must be sterilized in an autoclave at one atm for 10 minutes. after which it is stored for one year at a temperature from M. to + 10 ° C.

An estericide-based isoniazid preparation is administered in a warm form (37 ° C) subcutaneously in the area of the middle third of the neck at the rate of 10 mg / kg body weight according to the isoniazid content with an interval of 8-10 days for 35 months

In an experiment on calves, when they were infected with a virulent culture of the causative agent of tuberculosis and were treated with isoniazid on an etericide for 3 months with a 40 interval of 10 days, they did not become ill with tuberculosis, and when they were killed, they were also subjected to laboratory tests (bacteriological and biological tests on marine pigs) the initial culture of the causative agent of tuberculosis from them is not 45 allocated.

The effectiveness of the proposed method for the prevention of tuberculosis of calves was studied in a controlled epizootological experiment on calves obtained from cows with tuberculosis, with isolated cultivation of calves on a specialized farm from 7-10 days of age. In the group of 50 t, treated orally according to V.I. Rotov. 13.2% were detected. and in the group of 50 bodies of t treated by the proposed method, 2% of sick bodies of t.

The proposed method for the prevention of tuberculosis allows calves to protect (calves from tuberculosis if they are infected with a virulent culture of bovine species. During this time, calves infected with a bovine virulent culture but not undergoing chemoprophylaxis develop. severe forms of generalized tuberculosis. The use of an isteriazide-based isoniazid preparation by the subcutaneous method makes it possible to accurately dose it. The drug is administered easily, after subcutaneous administration it rapidly dissolves c, left no tumors.

The drug has pronounced bacteriostatic properties in the body of the body during 8 days, which was determined during a blood test according to the method of Rudoy N.M. (1975).

Claims (1)

The claims A method of preventing tuberculosis of calves, comprising administering a preparation containing isoniazid and a base, characterized in that, in order to accelerate absorption and increase prophylactic activity, an etericide is used as the basis for administration of the preparation in the following ratio of components: isoniazid - 100-200 g; ekteritsid - 1 l, moreover, the drug is administered subcutaneously at a dose of 10 mg / kg body weight according to the content of isoniazid with an interval of 8-12 days for three months.