RU1809822C - Method of water, soil and surfaces treatment from oil and petroleum products pollution - Google Patents

Abstract

Usage: purification of water, soil and surfaces from oil pollution. SUBSTANCE: natural selection strain of bacteria Acinetobacter deovbrun TsMPM-1878 in an aqueous solution of nitrogen-phosphorus additives is used for purification. 1 tab.

Description

The invention relates to methods for treating water, soil and surfaces and can be used to clean up oil spills and oil products.

The aim of the proposed method is to increase the cleaning efficiency, improve environmental and hygienic safety.

This object is achieved in that in the method of purifying water, soil and surfaces from contamination with oil and oil products, including the introduction of an aqueous solution of a natural strain of a bacterial culture in a mixture with mineral additives containing nitrogen and phosphorus, the strain Acinetobacter oleovprum VSB is used as a bacterial culture - .712 TsMPM-1878, and mineral additives contain nitrogen in ammonium form with an element concentration in solution from 1.0 mg / l to 1200 mg / l and phosphorus in phosphate form with an element concentration from 15 mg / l to 2500 mg / l ,

The use of a natural selection strain Acinetobacter

oleovorum VSB-712 in a solution with mineral additives containing nitrogen in ammonium form and phosphate in the form of phosphates, for example, fertilizers Ammonium sulfate GOST 9097-82 with nitrogen content 21% and Double superphosphate GOST 16306-89 with P20b45% content. allows optimizing the conditions for the existence of bacteria and improving the assimilation of hydrocarbons. With a decrease in the concentration of nitrogen and phosphorus below 10 mg / L and 15 mg / L, respectively, a significant decrease in the specific growth rate due to limitation is observed, with an overestimation of the upper boundaries of the above ranges, a marked inhibition of the development of bacteria is observed.

The method is carried out as follows.

In order to obtain the biomass of cells of the Acinetobacter oleovorum strain VSB-712, the culture was grown on a liquid nutrient medium of the following composition:

 (NHn) 2S04 3.5 kg / m:: KH2RO - 6.5. kg / m3; MgS04 - 0.7 kg / m3: FeSO-r 7H20 | §

about

lOO

th

with

12.5 g / m3; MnSCM SHaO - 12.5 g / m3; Znso

7H20 - 12.5 g / m3, CuSOo 5H20 - 3 g / m3.

As the sole carbon source, n-paraffins obtained by carbamide dewaxing with a high content of aromatic hydrocarbons are used.

The cultivation process is carried out continuously or intermittently at a temperature of from 20 ° C to 42 ° C and a pH of 6.5-7.2 under aerobic conditions.

If necessary, the resulting biomass of bacteria can be dried on a spray dryer.

The preparation of the mixture before use consists in the homogeneous dilution of biomass with an aqueous solution of nitrogen-phosphorus mineral additives.

Example. There was a contaminated raw aromatic Nizhnevartovskaya

 4 ha of soil with an intensity of pollution of about 20 kg / m by oil (the total amount of pollution in the area was about) ..

The treatment was carried out with an aqueous solution containing 75 g / m3 of ammonium sulfate (GOST 9097-82), 150 g / m3 of double superphosphate (GOST 16306-89) and 1.5 kg / m3 of dry biomass of bacteria Acinetobacteroleovorum VSB-712 (cell titer in solution about 1.5:

108 cells / ml). The flow rate of the solution is 10 m3 / ha.

After 45 days, the intensity of pollution decreased to 1 kg / m2; intensive germination of the grass cover was observed.

Example 2. A settling tank for collecting effluents of hydrocarbon-based drilling fluids with a mirror area of about 1 ha and a filling depth of about 1.5 m is contaminated with crude aromatic Nizhnevartovsk oil. .

The oil film thickness is about 1.8 cm, i.e. pollution intensity is about 15 kg / m2 (total amount of oil is about 150 tons).

The treatment was carried out with an aqueous solution containing 75 g / m ammonium sulfate. (GOST 9097-82), 150 g / m3 of double superphosphate (GOST 16306-89) and 1.0 kg / m3 of dry biomass of bacteria Acinetobacter oleovorum VSB-712 (titer of cells in solution about 108 cells / ml). The flow rate of the solution is 10 m ° / ha.

After 30 days, the surface of the sump was cleaned of the oil film.

PRI me R 3. There was a tank with a volume of 4.5 m3 for storing diesel fuel. Contaminated with sediments of paraffins and asphalts.

The total amount of pollution was estimated at 60-80 kg.

A 4 m solution containing 4 kg / m3 of ammonium sulfate (GOST 9097-82), 7 kg / m3 of double superphosphate (GOST 16306-89) and 1 kg / m3 of dry biomass of bacteria Acinetobacter oleovorum VSB-712 (4) were poured into the tank the titer of cells in solution is about 108 cells / ml),

The cultivation was carried out under aerobic conditions (the bubbling rate was 0.5 m3 air / m3 water min) for 10 days at a temperature of 20 ° C.

As a result of the process, the interior surfaces of the tank were cleaned of deposits.

5 Example 4. According to the results of laboratory tests, additives of nitrogen and phosphorus significantly increase the degree of utilization of oil distillates.

Tests were carried out in 0 rocking flasks at a temperature of 30 ° C.

Per 100 ml of tap water was added mg of ammonium sulfate (GOST 9097-82) and 15 mg of double superphosphate (GOST 16306-89) and 0.15 g of dry bacterial biomass 5 Acinetobacteroleovorum VSB-712 (cell titer in solution of about 1.5 108 cells / ml).

As pollution was used 1 ml of aromatic Nizhnevartovsk oil ... . . :.

0 nitrogen and phosphorus were not added to control flasks.

The results of the experiments on the third day are presented in table. 1 (averaging over 5 repetitions) .:

5 From the analysis of the results it follows that the effectiveness of the method, superior to the prior art, can be achieved only by using salts in nitrate form. 0 Thus, the proposed technical solution is new, because it is not known from the prior art, industrial applicable, which is confirmed by examples of its implementation in commercial conditions, it has an inventive step, since the technical result achieved by its implementation surpasses that found in the prior art.

0 Form of invention

A method of purifying water, soil and surfaces from contamination with oil and oil products, comprising introducing into the contaminated medium an aqueous solution containing a microorganism culture and mineral additives with nitrogen and phosphorus, characterized in that the bacteria Acinetobacter oleovorum TsMPM-1878 are used as microorganisms, while mineral additives contain

nitrogen in ammonium form with a nitrogen content of 10-1200 mg / l, and phosphorus in phosphate

form - with a phosphorus concentration of 15-1500 mg / l.

Conditions

% utilized oil hydrocarbons

With the addition of ammonium sulfate and double superphosphate

Without additives

With the addition of nitroammophoski (100 mg / l)

% recycled aromatics

83.7

38,4

36,4