RU1802701C - Device for surgical extraction of sex cells from pigs - Google Patents

Description

The invention relates to -experimental physiology and is intended for

the liver of ova from the uterus of pigs.

| An object of the invention is the possibility of extracting egg cells from pigs in a detailed aspect. .

The drawing shows the proposed Guo, a General view. The device includes a catcher catheter 1 and a two-channel catheter 2. The catcher catheter 1 has a locking ring 3 with holes 4 v a nozzle 5 to which a catheter 2f consisting of a tube 6 and a smaller dimmeter located inside its second tube 7 is connected, which, at one end 8, passes through the nozzle 5 into the trap and the head 1. On top of the catheter, the restriction ring 9, the felt first loop 10 and the outer flange 11 are fixed on the catheter with bolts 12 and two semi-oval plates 13 inserted hole in fl NCU 14.

The free ends of both catheter tubes are closed with plugs 15 and 16.

The capture head 1 with the retaining ring 3 and the nozzle 5 is a one-piece construction made of organic glass, and the two-channel catheter 2 connected to the head nozzle is made of polyvinyl chloride tube 6, inside which there is a second tube of smaller diameter 7 from the same material. A restriction ring 9 made of organic glass and a ribbon of medical felt 10 are fixedly fixed on the catheter, as well as the outer flange 11 is fixed to the catheter by means of bolts 12 screwed into the holes in the flange and two semi-oval metal plates 13 inserted inside the flange.

To use the proposed device for the purpose of extracting eggs from pigs, in the area of a hungry pit,

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about

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3

with

laparotomy in compliance with all the rules of surgical intervention. After dissection of all layers of the abdominal wall and peritoneum, the uterine horn is sought and removed to the outside. Indents 3-4 cm from the fallopian tube from the side of the greater curvature of the horn make a transverse incision to the opposite wall of the horn. Then, a two-story suture is applied to the incision in the caudal part of the uterine horn, and a capture head 1 is inserted into the opening of the cranial part of the horn, which is fixed there by suturing the free edge of the uterine horn through the holes 4 in the head ring 3. After suturing the peritoneum, several centimeters recede from the edge of the incision and do counter-puncture in the abdominal wall; through which the free end of the catheter is removed with a clamp, while the restrictive ring 9 and the felt loop 10 are located under the abdominal wall. Then, the aponeurosis of the abdominal muscles and nodular suture is sutured with a continuous suture - skin. Remove stitches for 8-10 days. On the outside of the abdominal wall on the catheter 2, the flange 11 is fixed using bolts 12, which, when twisted through a semi-oval plate. 13 squeeze the catheter 2, while leaving the lumen of the channel free. A restrictive ring 9 and a felt loop 10, which over time grows with fibrin and connective tissue, as well as the outer flange 11 do not allow the catheter 2 to move freely in the abdominal cavity, which prevents the device from tearing off the uterine horn. After the operations are completed and the next three days, a solution of furatsilin (1: 5000) is passed through the device once a day: Thereafter, the device is washed once a day with sterile physiological saline or Dulbecco's fluid; regardless of the physiological state of the ovaries. The device is washed and the eggs are washed out using a syringe, which is connected to the thin tube of the catheter 7, and the washing medium is collected in a receiving vessel through a tube with a large diameter.

Before use, the device is stored in 70 ° alcohol, and immediately before surgery, it is thoroughly washed with sterile saline.

Based on experiments conducted on 3 repair pigs and 2 main sows of large white breed, 2 repair pigs of Estonian bacon and 1 large black breed, it was found that using the proposed device, in comparison with the prototype, does not cause painful irritation , side effects and postoperative complications in experimental animals and allows

receive eggs from the same pigs during the period of several ovulations. This makes it possible to conduct a quantitative and qualitative assessment of oocytes in the age aspect, as well as after exposure to

an organism of various endogenous factors, drugs and biologically active substances, which allows the use of this device for scientific and educational purposes.

..

Claims (1)

The claims A pig egg retrieval device comprising a catheter comprising of two telescopic tubes, at the working end of each of which holes are made, and means for fixing the catheter in the abdominal wall and on the skin surface, characterized in that, in order to increase operational efficiency, it is equipped with a catching head, which has the shape of a glass with a hole made in its bottom, along the edge of which the connection is fixed from the outside a pipe, and an annular rim with holes is coaxially mounted on the side walls of the glass, while the catheter fixation device in the abdominal wall is made in the form of a restrictive ring and felt loop fixed to the outer tube, and the catheter fixation device on the skin surface is made in the form of an installed on the outer tube with the possibility of moving along the outer flange.