RU1793878C - Method of cabbage protection against bacterial sickness - Google Patents

Abstract

Usage: agriculture, plant protection, in particular cabbage, from bacterial diseases. SUMMARY OF THE INVENTION: Cabbage seeds are hydrothermally treated, then treated with a suspension of the bacterial strain Pseudomonas sp. VKPM B-3481 at a concentration of 106-109 cells / ml, seeded in a greenhouse. Before planting in the field, the root system of the cabbage seedlings is treated again with a bacterial suspension of the above strain, and then in the head formation phase. Treatment with a bacterial suspension reduces cabbage disease by vascular and mucous bacteriosis by 13 times. 1 tab.

Description

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The invention relates to the field of agriculture and can be used to protect cultivated plants, in particular cabbage, from bacterial diseases.

Cabbage is affected by numerous microorganisms, among which pathogens of vascular and mucous bacterioses are of great importance. Loss of uroho from these diseases can reach 100%.

Known methods for protecting cabbage from bacterioses are by treating the seeds with TMTD and phytolavin-Yu.

The closest in technical essence to the claimed method is hydrothermal treatment, which consists in immersing seeds in water at a temperature of 50 ° C for 30 minutes.

A common disadvantage of known methods of antibacterial treatment of seeds of cabbage, including the prototype (hydrothermal

treatment) consists in the fact that they do not protect the seedlings, and subsequently the plants from external infection transmitted through plant debris, soil, weeds and insects.

The aim of the invention is to increase the effectiveness of protecting cabbage from bacteriosis by suppressing external infection.

This goal is achieved using a suspension of cells of the bacterial strain Pseudomonas sp. AP33 (deposited in VKPM at the All-Russian Research Institute of Genetics under No. B-3481) at a concentration of 10 -109 cells / ml for seed treatment after preliminary hydrothermal treatment of the root system of seedlings before planting in open ground and spraying plants in the phase of head formation.

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At the same time, bacteria of this strain, which possess strong colonizing ability, multiply on the surface of plants and inhibit the development of pathogens of vascular and mucous bacterioses due to the synthesis of siderophores.

The use of a suspension concentration of less than 106 cells / ml leads to a decrease in the effect, and an increase in concentration above 109 cells / ml does not increase the effectiveness of the protective effect.

Example. After hydrothermal treatment, cabbage seeds were treated with a bacterial suspension of the strain Pseudomonas sp. AP33 at a concentration of 109 cells / ml at a rate of 20 ml per 1 kg of seed and immediately sown in a film greenhouse. Before planting in the field, the root system was treated in a bacterial suspension with a concentration of 107 cells / ml. In the head formation phase, plants were sprayed with a bacterial suspension of Pseudomonas sp. ARZZ with a concentration of 106 cells / ml. In other embodiments, the seeds were hydrothermally treated with phytoavin-100 and left untreated.

Claims (1)

SUMMARY OF THE INVENTION A method for protecting cabbage from bacterial diseases, comprising treating the seeds, root system of seedlings and plants during the head formation with an antibacterial agent, characterized in that, in order to increase the efficiency of the treatment of seeds and plants of cabbage with a bacterial suspension of Pseudomonas sp. AP33 for the development of vascular and mucous bacterioses In each variant, 100 plants were analyzed. The results are presented in the table. The results of the experiment show that the use of a bacterial suspension of Pseudomonas sp. AP33 reduces the development of vascular and mucous bacteriosis by 4 and 13 times, respectively: compared with known methods of protection. The yield of heads of cabbage in the variant using a bacterial antagonist was 11% higher compared to the prototype. However, the positive effect is much higher, since in the proposed embodiment, the affected ™ head of bacteria is significantly reduced by bacterioses, which will reduce waste during storage of products. Thus a positive effect The proposed method can be obtained by increasing the yield of cabbage, improving their quality and keeping quality during storage. The proposed method will allow to increase the efficiency of protection of cabbage from bacterial diseases by 2-3 times in comparison with the prototype. shields, as an antibacterial agent, a suspension of the bacterial strain Pseudomonas sp. VKPM B-3481 at a concentration of 106-109 cells / ml, while the seeds are subjected to hydrothermal treatment before seed treatment with an antibacterial agent.