RU1790603C - Method of post-yeast home-brewed beer processing - Google Patents

Abstract

Usage: waste water disposal by hydrolysis, pulp and paper industry. Essence: a continuous process of cultivating microorganisms of activated sludge under conditions of aeration in a fermenter at 35-39 ° C, with continuous selection of excess bacterial biomass from the foam phase at a rate of 0.037-0.06 from the non-aerated zone of the utilized ice yeast mash with speed 0.3-0. Ztab.

Description

The invention relates to methods for processing wastewater by the hydrolysis, pulp and paper industry,

A known method of processing post-brew mash by introducing activated sludge microorganisms into it, followed by continuous cultivation during aeration and selection of culture fluid.

The method is carried out at a temperature of 30-32 ° C, pH 7.0 in a fermenter at the same concentration of active biomass or throughout the aeration zone.

At the same time, part of the concentrated biomass is returned to the yeast-growing apparatus to maintain the biomass concentration necessary for cleaning the post-yeast mash, the remaining part of the biomass is sent for further thickening.

Obtained by the disposal of organic substances, biomass contains 39-40% of true protein.

The disadvantages of this method are the complexity of the process, the low quality of the resulting biomass.

The purpose of this method of processing post-yeast mash is to enrich the biomass of microorganisms with protein while simplifying the method.

This goal is achieved in that the cultivation is carried out at a temperature of 35-39 ° C, aeration is carried out in such a way as to ensure separation of the culture fluid into two layers with respect to the air flow, and the selection of the culture fluid is carried out from the upper and lower layers at a speed of 0.37-0.0604 and 0.30-0, respectively.

The process of processing organic substances of the post-yeast mash is carried out in airlift fermenters with a raised air distribution system and two extraction lines, one of which is located above and the other below the level of air supply. During aeration of the post-yeast brew above the supply level, a foam phase is formed in which

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microorganisms with flotation ability are retained. Macroorganisms and microorganisms growing at low speeds, non-floaters, are washed out of the apparatus by means of a bottom fermenter selection with a post-yeast brew. The selection of the culture fluid from the lower layer allows to obtain a biomass with a higher protein content by washing out the activated sludge with a low protein content, for example, protozoa, algae by lower sampling of microorganisms, increasing the content of bacterial cultures with the highest growth rates and high protein content . In this regard, the rate of utilization of PDB organic substances increases, the protein content in the biomass increases. The selection of biomass from the upper layer at a rate of 0.037-0.064 allows you to maintain the biomass concentration necessary for utilization of PDB organic substances without using a known method - recirculation of a part of the condensed active sludge, which can significantly reduce the age of the biomass. The rejection of recirculation also makes it possible to greatly simplify the process.

The simplification of the process is achieved by the fact that the proposed process is carried out at elevated temperatures of 35-39 ° C, thereby reducing the cost of cooling the substrate. An increase in temperature also influences the formation of microflora in the yeast-growing apparatus, promotes the growth of bacterial cultures and the inhibition of activated sludge microorganisms. The content of bacterial cultures and protein in biomass increases.

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Example 1. Post-yeast mash (PDB) with a concentration of BPCz - 6 g / l is fed into an airlift fermenter with a volume of 1250 m3 with a raised air distribution system and two sampling lines, one of which is located above and the other below the level of air supply .

During aeration of the post-yeast mash - above the level of air supply from the upper layer, culture fluid with D 0.0374 is taken from the non-aerated zone, utilized PDB is taken from growing D 0.15-0.34. The concentration of microorganisms in the aeration zone is 5 g / l (by a.s., c), the temperature of the process is 35 ° C.

The data are presented in table. 1.

Example 2, PDBs are disposed of under conditions similar to Example 1 by varying the selection of the biomass suspension from the lower layer and the selection of the utilized PDB with a D of 0.25.

The data are given in table. 2.

Example 3. PDBs are disposed of under conditions similar to Example 1 with a temperature ranging from 35 to 39 ° C. The selection of utilized PDB is carried out with D 0.25, the selection of a biomass suspension with D 0.05.

The data are given in table. 3.

According to the known method, the degree of utilization of organic substances of PDB corresponds to 50%, and the content of true protein is 37%, thus, the method of processing PDB in accordance with the invention can significantly increase the degree of utilization of organic substances and increase by 10% the content of instiny protein.

Simplification of the process is achieved by eliminating the step of cooling the substrate.

SUMMARY OF THE INVENTION A method for processing post-yeast mash, by introducing activated sludge microorganisms into it, followed by continuous cultivation during aeration and selection of culture fluid, characterized in that, in order to enrich the biomass of microorganisms with protein while simplifying the method, the cultivation is carried out at 35- 39 ° C, aeration is carried out so as to ensure the separation of the culture fluid into two layers with respect to the air flow, and the selection of the culture fluid is from the upper and lower layers with a speed of 0.037-0.060 and 0.30 - 0.15, respectively.

Table 1

Table 2

Table 3