RU1783390C - Of checking egg quality - Google Patents

Abstract

The invention relates to poultry farming and can be used in egg quality control. The time dependence of the shell fluorescence intensity is recorded, which is the sum of the exponentials. Intensity and decay times for each exponent are determined. These values are entered into the table and the degree of quality of the egg is determined. 2 il .. 1 tab.

Description

The invention relates to poultry farming,

A known method for controlling the quality of eggs by shell luminescence is associated with indicators of productivity, reproducibility, e-terrier and interior of birds. This method, based on a visual assessment of the color of eggs fluorescence emission (from bright crimson to dull yellow), is quite laborious, inaccurate, and depends on subjective factors.

Closest to the invention is a method based on recording the egg shell fluorescence intensity using a photomultiplier tube mounted in a luminescent microscope.

The known method contains the following operations: establish the test egg in the focus of a luminescent microscope; excite shell fluorescence by irradiating an egg with radiation from an excitation source; pass the fluorescence radiation of the shell through the interference

ate

filter at the input of the photoelectronic multiplier; registering an electrical signal at the output of the photomultiplier tube; comparing the received signal with a similarly received signal from a reference (barite plate); the relative egg fluorescence intensity indicates egg quality.

The disadvantage of this method is its low accuracy, associated with the use of three intensity classes (in arbitrary units) as a quantitative criterion: 10-59, 60-99, 100-200). In addition, this method is based on the assumption that the fluorescence intensity of the shell is constant over time. However, it is well known that the luminescence decays according to the exponential law.

The aim of the invention is to increase the reliability of the control when determining the degree of quality, as well as expanding the capabilities of the method based on register |

SO OJ

Cj

about

of the time dependence of the intensity of the fluorescence of the eggshell.

This goal is achieved by exciting fluorescence of the eggshell of the test egg located at the focus of the luminescent microscope, passing fluorescence radiation through an interference filter with a wavelength of 660 mm, recording the time dependence of the fluorescence intensity from the output of the photomultiplier in the recorder and analyzing the mathematical form of the time dependence of the fluorescence. Figure 1 shows a device for implementing the proposed method: 1 - a fluorescence excitation source; 2 - optical system; 3 - infrared filter; 4 - excitation filter; 5 - dividing plate; 6 - microscope objective; 7 - test egg; 8 - interference filter; 9 - photoelectronic multiplier; 10 - recorder; 11 - analyzer; figure 2 - dependence of the intensity of fluorescence of the shell from time to time.

The radiation of the fluorescence excitation source 1 passes through an optical system 2 forming a parallel beam, which passes through an infrared filter 3 and an excitation filter 4. Then, using the dividing plate 5, the radiation is fixed by the lens 6 to the surface of the test egg 7. The radiation of shell fluorescence. The egg passes in the opposite direction through the lens 6, the plate 5 and the interference filter 8, arriving at the input of the photomultiplier tube 9. An electric signal from the output of the photomultiplier tube is fed to the input of the recorder 10, on the tape of which the time course of the fluorescence intensity and the analyzer 11 are recorded.

A quantitative assessment of the quality of eggs is carried out by means of time dependence of the fluorescence intensity (t), which is described by the sum of two components:

l (t) - Ae + Be 1 / Ј,

(D

where A and B are the amplitudes; n and ta are the decay times for a rapidly and slowly decaying exponent, respectively. A typical time course of the egg shell fluorescence intensity is shown in FIG. 2. Four parameters A, n, B and Tg change depending on the color of the egg shell. In addition, the age of the egg also affects the numerical values of these parameters.

0

5 0 5

0 5 0

5

0

5

Thus, the proposed method contains the following operations: establish the test egg in the focus of a luminescent microscope; excite shell fluorescence by irradiating an egg with radiation from an excitation source; shell fluorescence radiation is passed through an interference filter to the input of the photoelectron multiplier; an electrical signal from the output of the photoelectronic multiplier is fed to the input of a recording device, for example, a recorder; record on the tape of the recorder the time course of the intensity l (t) of shell fluorescence; decomposing the total fluorescence intensity (t) into the sum of the elemental components; evaluate egg quality by the amplitude and time characteristics of the fast and slow exponents of the l (t) curve.

Example. Fluorescence-egg shells are excited by a DRSh-250-3 mercury lamp, the radiation of which is passed through an infrared filter (a layer of distilled water 5 cm thick) and excitation filters UFS-b and SZS 24. Radiation reflected from the ZhSZ + BS8 dividing plate passes through the lens L 10 x 0.40, exciting the fluorescence of the eggshell of the test egg. The fluorescence radiation passes through a lens, a dividing plate and an interference filter of 660 mm to the input of an FEU-79 photomultiplier tube, the output of which is connected to the input of an N-ZOb recorder,

Microscope diaphragm during operation 1.5. Recording speed 25 cm / s; gain 0.1 V / cm.

The results of recording the l (t) curves for eggs of different colors are tabulated.

As can be seen from the table, darker colors are characterized by large values of amplitudes A and B; parameter n decreases, and parameter n increases with darkening of the color.

A quantitative assessment of the color of the shell and, accordingly, the quality of the eggs can be carried out by distributing the values of A, n, B, t by bit, for example: AI 100 - 80; A2 79-50; Az 49 - 20; A 10-0; n - 30 - 30; ta 31 - 40; g3 - 41 - 50, etc.

Bit combinations AI, n. Bi. TI determines the degree of quality of a given egg.

Claims (1)

SUMMARY OF THE INVENTION A method for controlling the quality of eggs, including the excitation and detection of egg shell fluorescence and use in as a criterion for controlling the magnitude of the fluorescence intensity, characterized in that, in order to increase the reliability of the control when determining the degree of quality, the dependence of the shell fluorescence intensity on time l (t) and quantitative the quality of the eggs is assessed by analyzing the dependence l (t), which is represented as the sum of two exponential functions l (t) Ae t / 2v +, for which ri Г2, the quantities A, B, and P are used as quality criteria G2. in Q Editor G. Welsk Compiled by Yu. Posudin Tehred M. Morgenthal pu 2 Proofreader N. Gunko