RU1651555C - Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus - Google Patents

Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus

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Publication number
RU1651555C
RU1651555C SU894746353A SU4746353A RU1651555C RU 1651555 C RU1651555 C RU 1651555C SU 894746353 A SU894746353 A SU 894746353A SU 4746353 A SU4746353 A SU 4746353A RU 1651555 C RU1651555 C RU 1651555C
Authority
RU
Russia
Prior art keywords
hepatitis
virus
plasmid dna
recombinant plasmid
gene
Prior art date
Application number
SU894746353A
Other languages
Russian (ru)
Inventor
А.С. Беляев
И.П. Дмитриев
М.Ю. Рукавишников
А.Н. Синяков
Original Assignee
Всесоюзный научно-исследовательский институт молекулярной биологии Научно-производственного объединения "Вектор"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Всесоюзный научно-исследовательский институт молекулярной биологии Научно-производственного объединения "Вектор" filed Critical Всесоюзный научно-исследовательский институт молекулярной биологии Научно-производственного объединения "Вектор"
Priority to SU894746353A priority Critical patent/RU1651555C/en
Application granted granted Critical
Publication of RU1651555C publication Critical patent/RU1651555C/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Изобретение относитс  к медицине, биотехнологии и генетической инженерии и может быть использовано при получении живой рекомбинантной вакцины против гепатита В. Целью изобретени   вл етс  создание плазмиды, обеспечивающей экспрессию антигена вируса гепатита В в клетках млекопитающих.Рекомбинантна  плазмидна  ДНК р 7,5 С состоит из Sty 1 - фрагмента генома вируса гепатита В (ВГВ) дли- ной 575 п.о.. содержащего С-ген ВГВ без АТС кодона и большей части pre-облзг.ти (дл  обеспечени  инициаци  трансл ции непосредственно с ATG-кодона С-тена). и вектора р VAR 15, обеспечивающего интеграцию и экспрессию С-гена в составе гена тими- динкиназы вируса осповакцины ЛИВП.The invention relates to medicine, biotechnology and genetic engineering and can be used to obtain a live recombinant hepatitis B vaccine. The aim of the invention is to create a plasmid that expresses the hepatitis B virus antigen in mammalian cells. The recombinant plasmid DNA p 7.5 C consists of Sty 1 - a fragment of the genome of hepatitis B virus (HBV) 575 bp in length containing the HBV C gene without an ATC codon and most of the pre-regional hepatitis (to ensure translation is initiated directly from the ATG codon of C-ten) . and p VAR 15 vector, which provides for the integration and expression of the C gene in the thymidine kinase gene of the vaccine virus LIVP.

SU894746353A 1989-07-27 1989-07-27 Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus RU1651555C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU894746353A RU1651555C (en) 1989-07-27 1989-07-27 Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU894746353A RU1651555C (en) 1989-07-27 1989-07-27 Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus

Publications (1)

Publication Number Publication Date
RU1651555C true RU1651555C (en) 1993-07-07

Family

ID=21473161

Family Applications (1)

Application Number Title Priority Date Filing Date
SU894746353A RU1651555C (en) 1989-07-27 1989-07-27 Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus

Country Status (1)

Country Link
RU (1) RU1651555C (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Nature, 1983, v.. 302, p. 490-495. *

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