RU1651555C - Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus - Google Patents
Recombinant plasmid dna p7, 5c coding antigen hepatitis b virusInfo
- Publication number
- RU1651555C RU1651555C SU894746353A SU4746353A RU1651555C RU 1651555 C RU1651555 C RU 1651555C SU 894746353 A SU894746353 A SU 894746353A SU 4746353 A SU4746353 A SU 4746353A RU 1651555 C RU1651555 C RU 1651555C
- Authority
- RU
- Russia
- Prior art keywords
- hepatitis
- virus
- plasmid dna
- recombinant plasmid
- gene
- Prior art date
Links
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относитс к медицине, биотехнологии и генетической инженерии и может быть использовано при получении живой рекомбинантной вакцины против гепатита В. Целью изобретени вл етс создание плазмиды, обеспечивающей экспрессию антигена вируса гепатита В в клетках млекопитающих.Рекомбинантна плазмидна ДНК р 7,5 С состоит из Sty 1 - фрагмента генома вируса гепатита В (ВГВ) дли- ной 575 п.о.. содержащего С-ген ВГВ без АТС кодона и большей части pre-облзг.ти (дл обеспечени инициаци трансл ции непосредственно с ATG-кодона С-тена). и вектора р VAR 15, обеспечивающего интеграцию и экспрессию С-гена в составе гена тими- динкиназы вируса осповакцины ЛИВП.The invention relates to medicine, biotechnology and genetic engineering and can be used to obtain a live recombinant hepatitis B vaccine. The aim of the invention is to create a plasmid that expresses the hepatitis B virus antigen in mammalian cells. The recombinant plasmid DNA p 7.5 C consists of Sty 1 - a fragment of the genome of hepatitis B virus (HBV) 575 bp in length containing the HBV C gene without an ATC codon and most of the pre-regional hepatitis (to ensure translation is initiated directly from the ATG codon of C-ten) . and p VAR 15 vector, which provides for the integration and expression of the C gene in the thymidine kinase gene of the vaccine virus LIVP.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU894746353A RU1651555C (en) | 1989-07-27 | 1989-07-27 | Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU894746353A RU1651555C (en) | 1989-07-27 | 1989-07-27 | Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus |
Publications (1)
Publication Number | Publication Date |
---|---|
RU1651555C true RU1651555C (en) | 1993-07-07 |
Family
ID=21473161
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU894746353A RU1651555C (en) | 1989-07-27 | 1989-07-27 | Recombinant plasmid dna p7, 5c coding antigen hepatitis b virus |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU1651555C (en) |
-
1989
- 1989-07-27 RU SU894746353A patent/RU1651555C/en active
Non-Patent Citations (1)
Title |
---|
Nature, 1983, v.. 302, p. 490-495. * |
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