RU164145U1 - LIQUID PIPELINE IN COMPOSITE BIOLOGICAL REACTOR OF A PLANT FOR PRODUCING BIOMASS OF AEROBIC MICRO-ORGANISMS - Google Patents

Abstract

1. A liquid piping in a composite biological reactor of a plant for the production of biomass of aerobic microorganisms, characterized by the presence of the first and second pipelines, configured to interconnect the fermenter and an additional fermenter, where the first end of the first pipe is made with the possibility of connection with the lower channel of the fermenter, and the second end made with the possibility of connecting with the lifting channel of an additional fermenter, one of the ends of the second pipeline is made with pressure with the lower channel of the additional fermenter, and the second end of the second pipe is made with the possibility of connecting with the lifting channel of the fermenter, the first pipe is equipped with a circulation fluid culture fluid, positioned in it at the first end, and both pipelines are equipped with temperature, pH and CO sensors located in these pipelines in areas of the middle of their lengths, made with the possibility of connecting information and switching through an interface with an IBM compatible personal computer, on rum installed program "GAPRIN" 2 computers. The liquid pipe in the composite biological reactor of the plant for the production of aerobic microorganism biomass according to claim 1, characterized in that the pipe for the transfer of enriched culture fluid from the fermenter equipped with a shut-off device is connected to the second pipe, and the pipe for supplying mineral nutrients to the fermenter is connected to the first pipe, also equipped with a locking device. 3. Liquid conduit in a composite biological reactor for

Description

The utility model relates to the field of microbiology and can be used as a liquid pipe installation for the production of biomass of aerobic microorganisms.

The prior art fluid pipe in a composite biological reactor installation for the production of biomass of aerobic microorganisms (Invention of the Russian Federation No. 2322488, IPC: S12M 1/00, publ. 04/20/2008, Bull. No. 11). The known device consists of the first and second pipelines connecting the fermenter and the additional fermenter, while the first end of the first pipe is connected to the lower channel of the fermenter, and its second end is connected to the lift channel of the additional fermenter, one of the ends of the second pipe is connected to the lower channel of the additional fermenter, and the second end of the second pipe is connected to the lifting channel of the fermenter, and the first pipe is equipped with a circulation agent of the culture fluid ty positioned in it near the first end. In addition, in the known device, a pipe for connecting the enriched culture fluid from the fermenter equipped with a locking device is connected to the second pipe, and a pipe for supplying mineral nutrients to the fermenter, also equipped with a locking device, is connected to the first pipe.

The disadvantage of this analogue is the lack of means for monitoring the parameters of the culture fluid transported through the liquid pipe, which does not allow timely diversion of the enriched culture fluid with accumulated bioprotein from the composite fermenter, which causes a decrease in productivity.

The closest in technical essence and the achieved result to the proposed device is a well-known liquid pipe in a composite biological reactor installation for the production of biomass of aerobic microorganisms (Invention of the Russian Federation No. 2352626, IPC: S12M 1/02, publ. 04/20/2009, Bull. No. 11) . This device is adopted as a prototype device. The known prototype device is characterized by the design of the liquid pipe formed by the first and second pipelines connecting the fermenter and the additional fermenter, while the first end of the first pipe of the liquid pipe is connected to the lower channel of the fermenter, and its second end is connected to the lifting channel of the additional fermenter, one of the ends of the second the pipeline of this fluid pipe is connected to the lower channel of the additional fermenter, and the second end of the second pipe is connected to the lifting cable scarlet fermenter, wherein the first conduit is provided with a circulator culture fluid therein positioned near the first end. It matters that the pipeline connected to the second pipeline to drain the enriched culture fluid from the fermenter is equipped with a shut-off device, and the pipeline for supplying mineral nutrients to the fermenter, also equipped with a shut-off device, is connected to the first pipeline.

The disadvantage of the prototype device is the lack of means for monitoring the parameters of the culture fluid moving through the liquid pipe, which does not allow timely diversion of the enriched culture fluid from the composite fermenter. The latter is fraught with a decrease in productivity of bioprotein production.

The task to which the creation of the device proposed within the claimed utility model is aimed is to increase the level of efficiency of the use of fermenters in terms of increasing the yield of the target product (bioprotein) and saving energy and materials used for work, in particular carbon-containing raw materials (natural or liquefied gases).

The technical result expected from the use of the claimed device is to increase the productivity of bioprotein.

The claimed technical result is achieved by the fact that the liquid pipe in the composite biological reactor of the plant for the production of biomass of aerobic microorganisms consists of the first and second pipelines, made with the possibility of connecting a fermenter and an additional fermenter, where the first end of the first pipe is made with the possibility of connection with the lower channel of the fermenter and its second end face is made with the possibility of connection with the lifting channel of an additional fermenter, one of the ends of the second pipe wires made with the possibility of connecting with the lower channel of the additional fermenter, and the second end of the second pipe is made with the possibility of connecting with the lifting channel of the fermenter, and the first pipe is equipped with a circulation fluid culture fluid, positioned in it at the first end, and both pipelines are equipped with temperature sensors, pH and CO _2, arranged in said pipes in the middle zones of their lengths, adapted to the compounds information switchgear via an interface with IBM Combine th the personal computer, which is installed on the computer program "GAPRIN".

It is desirable that a pipeline for transferring enriched culture fluid from the fermenter equipped with a locking device be connected to the second pipe of the liquid pipe, and a pipe for supplying mineral nutrients to the fermenter also equipped with a locking device is connected to the first pipe of the liquid pipe.

Preferably, a valve is used as the locking device.

The claimed device is illustrated in the figure. In FIG. 1 conventionally depicted the claimed device (vertical section, side view).

The list of positions.

1. The fermenter.

1.1. The downstream channel of the fermenter.

1.2. The lifting channel of the fermenter.

2. Additional fermenter.

2.1. The lower channel of the additional fermenter.

2.2. The lifting channel of the additional fermenter.

3. The inducer of the circulation of the culture fluid.

4. Temperature sensor.

5. pH sensor.

6. CO ₂ sensor.

7. Information and switching channel.

8. Interface.

9. IBM compatible personal computer.

9.1. Display.

10. The first pipeline.

11. The second pipeline.

12. Pipeline for drainage of enriched culture fluid.

12.1. The locking device of the pipeline to drain the enriched culture fluid.

13. A pipeline for supplying mineral nutrients.

13.1. Pipeline shut-off device for supplying mineral nutrients.

The stimulator of the circulation of the culture fluid 3 (Fig. 1) in the proposed device can be made on the basis of a circulation pump model Wilo - Star-Z Nova /. It serves to move along the liquid pipe of the culture fluid between the fermenter 1 (Fig. 1) and the additional fermenter 2 (Fig. 1).

As temperature sensor 4 (FIG. 1), the analogue devices ADT7320 of the prior art can be used. A sensor for measuring pH 5 (Fig. 1) can be made on the basis of a Menosens sensor of the Orbipas CPE81D model. Accordingly, from a wide range of sensors for determining carbon dioxide concentration, a sensor 5 (Fig. 1) of the METTLEP TOLEDO brand JnPro 5000i series () can be used . The abovementioned sensors are arranged in pipelines essentially in zones of the middle of their lengths.

The choice of interfaces suitable for use in the proposed device is diverse // but it is advisable based on the properties of the computer program “GAPRIN” ”/ Certificate on state registration of the computer program №2015618884“ GAPRIN ”, the date of state registration in the Register of computer programs on August 19, 2015 d. / use interface 8 (Fig. 1) of the RS-232 model //.

As an IBM compatible personal computer 9 (Fig. 1) to equip the claimed device, you can use a computer model Advantice VL 310, with the ability to use a remote display 9.1 (Fig. 1), for example, the model ViewUPS-X remote LSD display /.

The first pipe 10 (Fig. 1) can be made of corrosion-resistant (with respect to the culture fluid) material, in particular, AISI 304 stainless steel. A similar material can also be used for the manufacture of the second 11 (Fig. 1) used in the design of the proposed device pipeline.

The pipeline for draining the enriched culture fluid from the fermenter 12 (Fig. 1), as well as the pipeline for supplying mineral nutrients to the fermenter 13 (Fig. 1) must also be corrosion-resistant (with respect to the culture fluid). Hence the need arises to use for their manufacture a material similar to the material for the manufacture of the first 10 (Fig. 1) and / or second 11 (Fig. 1) pipelines. In the simplest case, as a shut-off device 12.1 (Fig. 1) of the pipeline for draining the enriched culture fluid from the fermenter 12 (Fig. 1) and a shut-off device 13.1 (Fig. 1) of the pipeline for supplying mineral nutrients to the fermenter 13 (Fig. 1) ) can be used valves stainless models do. 80 ... 100 //.

The operation of the claimed device in dynamics is illustrated by an example.

Example.

In the present example, a fermenter 1 was used (Fig. 1), made in the form of a vertically oriented pipe with closed ends with a volume of 12 m ³ . Additional fermenter 2 (Fig. 1) had a shape similar to the main fermenter 1 (Fig. 1), while its volume was 6 m ³ . For the production of aerobic microorganisms, an initial culture fluid was used in which the initial content of the bacterial strain Methylococcus capsulatus VSB-874 / USSR Author's Certificate No. 770200, dated June 13, 1980) / amounted to 0.5% weight per 1 liter of liquid nutrient medium. The initial culture fluid based on the aforementioned strain of the bacterium Methylococcus capsulatus VSB-874 with a temperature of 49 ° C was pumped into the apparatus used to produce biomass of aerobic microorganisms through a pipeline for supplying mineral nutrients to the fermenter 13 (Fig. 1) with an open shut-off device (valve) 13.1 (Fig. 1). Simultaneously with the beginning of the injection of the initial culture fluid into the apparatus used for the production of biomass of aerobic microorganisms, the circulation fluid circulating agent 3 was launched (Fig. 1). Having thus pumped approximately 144 m ^{3 of} culture fluid (which corresponded to the filling of fermenter 1 (Fig. 1) and additional fermenter 2 (Fig. 1) for 80% of each of their volumes), the shut-off device (valve) 13.1 (Fig. 1) is closed . Received into the fermenter 1 (Fig. 1) through the lifting channel 1.2 (Fig. 1), the culture fluid is saturated with oxygen from the air supplied to it through a bubbler (not shown) and enters the lowering channel 1.1 (Fig. 1). Under the influence of the stimulator of the circulation of the culture fluid 3 (Fig. 1) and gravity, the culture fluid moves to the inlet of the first pipeline 10 (Fig. 1), which is one of the two structural elements of the claimed device. Passing through the first pipeline 10 (Fig. 1), the culture fluid washes installed in it, essentially in the middle of its length, temperature, pH and CO ² sensors, each of which reads the culture fluid parameter corresponding to its purpose and through the information and switching channel 7 (Fig. 1) through the interface 8 (Fig. 1) sends to processing the computer program "GAPRIN to the input of an IBM compatible personal computer 9 (Fig. 1). The results obtained are displayed (at the discretion of the installation operator), in absolute and / or analytical forms on the display 9.1 (Fig. 1). From the outlet of the first pipeline 10 (Fig. 1), the oxygen-enriched culture fluid enters the riser channel of the additional fermenter 2.2 (Fig. 1) where it is saturated with methane-containing gas (using natural gas) using an appropriate bubbler (not shown). After saturation in the lifting channel of the additional fermenter 2.2 (Fig. 1) of the culture fluid with a carbon source, the exponential phase of the growth of the biomass of aerobic microorganisms begins, which is accompanied by an increase in the temperature of the culture fluid, as well as an increase in the content of CO ² and a change in the pH value (as a result of the receipt of products metabolism into the culture fluid). In this state, the culture fluid enters the lower channel of the additional fermenter 2 (Fig. 1), and from it moves to the inlet of the second pipe 11 (Fig. 1) of the claimed fluid pipe. As in the case with the passage of the culture fluid of the first pipe 10 (Fig. 1), the culture fluid moved through the second pipe 11 (Fig. 1) of the fluid pipe washes the temperature, pH and CO ² sensors. Similarly to the above, information received from these sensors is displayed, at the discretion of the installation operator, in absolute and / or analytical forms on display 9.1 (Fig. 1).

Having passed the exit of the second pipeline 11 (Fig. 1), the culture fluid again enters the lifting channel 1.2 (Fig. 1) of the fermenter and the production of biomass of aerobic microorganisms is repeated. Since in the fermenter 1 (Fig. 1) and additional fermenter 2 (Fig. 1), the culture liquid is separately saturated with oxygen and methane (coming from the air pumped into the bubbler of the fermenter 1 (Fig. 1) and the natural fermenter pumped into the bubbler gas), the culture fluid characteristics in terms of temperature, pH, and CO ² in the step of the exponential growth of the biomass taken from the respective sensors placed in the first 10 (FIG. 1) and second 11 (FIG. 1) pipeline zhidkosteprovoda at creatures in areas of the middle of their lengths will vary. And vice versa, leveling the readings of the above-mentioned sensors, placed both in the first 10 (Fig. 1) and in the second 11 (Fig. 1) pipelines of the declared liquid pipeline means the completion of the stage of exponential growth of biomass and the emergence of necessity (due to a decrease in the rate of thickening of the culture fluid with biomass) drainage of the condensed culture fluid from the installation for subsequent isolation of the bioprotein from it. Having recorded the completion of the process of exponential growth of biomass by reaching the minimum value of the difference in the readings of both groups of sensors, the shut-off device of the pipeline to drain the enriched culture fluid 12.1 is opened (Fig. 1) and the latter is poured, for example, into a centrifugal separator (or other device involving separation crude bioprotein from the liquid). Due to the fact that the air originally used as a source of oxygen, as well as natural gas originally used as a methane source, has although insignificant (each up to 5-6%) variations in its characteristics (for example, relative humidity, by air), the time for the exponential growth stage to complete biomass can fluctuate up to 10%. The table presents comparative data on the biomass productivity in a composite biological reactor (fermenter) when using the proposed device and the prototype device as a liquid pipe.

As follows from the data reflected in the Table, the proposed device ensures the achievement of the claimed technical result.

For the manufacture of the claimed device can be used known devices and mechanisms, materials, components and accessories, which is evidence of compliance of the proposed utility model with the patentability criterion of "industrial applicability"

Claims (3)

1. A liquid piping in a composite biological reactor of a plant for the production of biomass of aerobic microorganisms, characterized by the presence of the first and second pipelines, configured to interconnect the fermenter and an additional fermenter, where the first end of the first pipe is made with the possibility of connection with the lower channel of the fermenter, and the second end made with the possibility of connecting with the lifting channel of an additional fermenter, one of the ends of the second pipeline is made with of the connections standpipe channel additional fermenter, and the second end of the second conduit is configured to couple to the lift channel of the fermenter, wherein the first conduit is provided with a circulator culture fluid positioned therein at the first end, and both lines are equipped with temperature sensors, pH, and CO ₂ arranged in these pipelines in areas of the middle of their lengths, made with the possibility of connecting information and switching through an interface with an IBM compatible personal computer, on which Tator installed the computer program "GAPRIN." 2. The liquid piping in the composite biological reactor of the apparatus for the production of biomass of aerobic microorganisms according to claim 1, characterized in that the pipe of the enriched culture fluid from the fermenter equipped with a shut-off device is connected to the second pipe, and the pipe for supplying mineral nutrients to the first pipe is connected fermenter also equipped with a locking device. 3. A liquid conduit in a composite biological reactor of an apparatus for producing biomass of aerobic microorganisms according to claim 2, characterized in that a valve is used as a shut-off device.

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