RU163772U1 - FLOATING DISASSEMBLY DEVICE FOR MONITORING MICROFLORA OF OPEN WATER AND WASTE WATER - Google Patents

Abstract

1. Floating collapsible device for monitoring the microflora of open water bodies and wastewater for epidemiological, microbiological and environmental monitoring of the microflora of open water bodies, characterized in that it is made in the form of a floating collapsible disk container with a membrane filter, consisting of a lid and a disk base with a protrusion of the outlet pipe through the aluminum channel pipe in the center of the base of the device, 3 floats are installed on the frame of the float platform of the disk container, and on the float s platform polikanalnoy magnetic trap with cylindrical recesses for magnets and magnoimmunosorbentov niobium Sampler slot 2 has a float which floats are movable and fixing to change the depth of immersion of the container and the liquid level above the surface membrany.2. The device according to claim 1, characterized in that its floating collapsible disk container, as well as a floating platform with a multi-channel magnetic trap and a slotted sampler, are made with the possibility of installation on the surface of the reservoir due to movable brackets using a radio-controlled floating means or telescopic pole. 3. The device according to claim 2, characterized in that guide rods and a “lock” are installed between the disk base and the lid of the disk container. The device according to claim 3, characterized in that the strengthening of the mechanical strength of the filter membrane is ensured by the installation of a supporting metal mesh resting on the edge of the cutout around the circumference of the disk base and the protrusion of the pipe of the liquid-discharge channel in the center of this

Description

The floating collapsible device for monitoring the microflora of open water bodies and wastewater (hereinafter the device) refers to technical devices for providing microbiological, epidemiological and environmental monitoring of the microflora of open water bodies and wastewater.

The pollution levels of open water bodies and wastewater depend on the climatic seasonal fluctuations of the weather and the accompanying meteorological manifestations, primarily rainfall, floods, ambient temperature, and solar activity. Storm and flood waters contain tens and hundreds of times higher concentrations of microorganisms in terms of unit volume of liquid, compared with those in the absence of meteorological activity (Leptospirosis of humans and animals / Ed. By Prof. V.V. Ananyin. -. - M .: “Medicine” - 1971. - 352 p.).

The proposed device can be used for mechanical capture of microorganisms by the filter membrane of the intake device during forced pumping (aspiration) of liquid from open reservoirs and wastewater. After trapping and adsorption of microorganisms on the membrane, the latter is subjected to bacteriological analysis with microscopy of smears and inoculation of the wash from the filter onto nutrient media. As filters, “Vladipor Membranes”, MFA-A, No. 2, TU 6-05-1924-82, products of the Kazan Tasma Production Association with a pore size of 0.35-0.50 µ were used. (microns).

In the specialized literature, various filtering devices are used that are used in the study of water in laboratory conditions.

Known filter apparatus Rublevsky waterworks, which consists of two parts. The upper part is a glass funnel with a capacity of up to 600 ml. A metal ring with two grooves (recesses) is dressed on the neck of the funnel. The lower part of the apparatus - a metal filter table - has two protrusions in the form of a part of a screw thread and a ceramic porous plate on which a membrane filter is placed during filtering (http://ru-ecology.info/term/39868/;

http://ru-ecology.info/static/pngbig/297405162.png).

The main disadvantages of the filter apparatus of the Rublevskaya waterworks station is that it is used in monitoring the tap water of large settlements only in laboratory conditions.

The above disadvantages, as well as the fragility of the glass structure of the apparatus and its cumbersomeness, are obstacles when using microflora of open water bodies and sewage as a filtering device (Skvortsov V.V., Kiktenko V.S., Kucherenko V.D. Survival and indication of pathogenic microbes in the external environment. - M.: Medicine, 1966. - 360 p.).

Closest to the claimed utility model is the Seitz apparatus, in which water is filtered through a membrane filter located on a metal mesh at the base of a metal cup with the application of external energy to filter it.

The main disadvantages of the Zeitz filter apparatus are the limited possibility of its use - only in laboratory conditions and difficulties arising in the study of large volumes of water (> 500 ml), as well as the fragility, bulkyness and absolute inability of the apparatus for use in the field when monitoring the microflora of open water bodies, wastewater and the use of magnetic immunosorbents (Skvortsov V.V., Kiktenko V.S., Kucherenko V.D. Survival and indication of pathogenic microbes in the external environment. - M .: Medicine, 1966. - 360 s; Specific Indication of Pathogenic Biological Agents. Practical Guide. / Ed. by G. G. Onishchenko. M: ZAO MP Hygiene, 2006. 288 p.).

The purpose of the claimed utility model is to increase the efficiency of trapping microorganisms from open water and wastewater during microbiological, epidemiological and environmental monitoring with minimized traumatic effects on microbes due to the sparing conditions for sampling water, concentrating cells on the surface of the membrane filter and / or their sorption on the surface magnetic immunosorbents (MIS).

The essence of the technical solution of the utility model is to sparingly take water samples from the surface of the reservoir, the possibility of delaying microorganisms on the membrane filter and / or their selection on magnetic immunosorbents with the ability to control the immersion depth of the functional units of the device (1-5 mm) with a forced flow of fluid.

The general concept of designing the main components of the device consists in the possibility of changing the immersion depth of its water intake part due to engineering solutions, and the functional features of the device, despite some structural differences in their components, are aimed at achieving the goal of creating and justifying a technical solution for a utility model with the possibility of using, not only the concentration of microorganisms on the membrane filter and the direct sampling of water samples into a container, but also modern methods of selective concentration of micro MIS-organisms on.

Despite the diverse nature of the functional support for sampling when monitoring the microflora of open water bodies and wastewater, the multifunctionality of the utility model is ensured by the devices included in the device.

Thus, the proposed device allows you to:

- concentrate microorganisms on a membrane filter;

- selectively concentrate microflora, its antigens and DNA on MIS;

- take samples of liquid in the tank.

The mass of the device to ensure each of the above functions, taking into account the use of various devices, does not exceed 80 g.

The device for microflora concentration on the membrane filter is a flat disk container, the body of which is formed by a disk base with a rod - a “lock” and a lid in the form of a washer with 3 fixing rods evenly spaced around the edge of the circle. The disk base and the cover are hermetically connected with the help of a covering sealing elastic rubber cuff, which presses the membrane filter, which delimits the filter chamber from the water surface at a depth of 1-5 mm due to floats that regulate the level of immersion of the container in water, and to prevent violation of the integrity of the membrane filter under the last is a small metal support grid in the form of a disk, the edge of which and the center, respectively, rely on the inner protrusion of the disk base channel protrusion with a screwing in aluminum pipe (M8 × 0.75) with a diameter of 8 mm with 4 additional side openings with a diameter of 2 mm for draining the liquid from the container, and the fragment of the pipe emerging from the chamber is bent at right angles and serves to connect an elastic flexible silicone tube and ensure fluid flow through a peristaltic pump or aspirator.

To optimize the function of the device, a 5-mm-thick organic glass is used to make a disk-shaped matrix, the circumferential surface of which is bounded by a metal strip with a 2 mm protruding cutting edge, and 4 metal pipes with 2 mm protruding above its surface sharpened edges and placed congruently to the 3 fixing rods and a “lock” for cutting membrane filters of the required size from a sheet blank of the “Vladipor Membrane” type MFA-A No. 2 with a diameter of 293 mm TU 6-05-19248-2, produced dstva "Tasma", Kazan.

To install a rubber sealing cuff around the circumference of a disk container case, a handle with a U-shaped “fork” fixed to it with 2 parallel rods 70 mm long was made from a steel strip with a thickness of 6 mm and a rod 4 mm in diameter, which served to simplify the preliminary fixation of the sealing elastic rubber cuff and its installation on the side surface of a flat disk container.

For storage of sterile and used membrane filters, modified containers with magnetic caps have been manufactured. The container consists of a rectangular base with a recessed lodgement for filters and a lid. The base and cover are fixed by a niobium magnet with dimensions of 3 × 3 mm and a steel strip of 5 × 10 mm, respectively, fixed in the base and cover.

Before starting work, a steel support mesh is installed between the base and the lid of the disk container of the device, over which a membrane filter with holes is carefully placed congruently to the guide rods and the “lock”, the device chamber is sealed with a rubber cuff using a handle with a spacer, and a float platform is inserted from the bottom of the device with 3 floats and a slight clockwise rotation, which is fixed with spring clips to the bottom of the device. The internal volume of the chamber of the disk container of the device through the connector of the outlet silicone tube is completely filled without the formation of air bubbles with sterile saline. The container with floats is placed in a tank with tap water and by moving the floats relative to the container, the horizontal level of immersion of the surface of the membrane filter to a depth of 1 ÷ 5 mm from the surface of the liquid is established, being guided by the uniformity of the spreading of water along the surface of the lid and its thickness equal to 3 mm. The outlet pipe connector is connected to a fluid flow inducer — an aspirating channel of a peristaltic pump (or a manual aspirator), which is turned on for 8-10 seconds to check the system for leaks and to estimate its flow rate at a rate of 8-10 ml / sec, with a minimum installation level of the peristaltic pump relative to the surface of the water 0.15-0.20 m to prevent the negative influence of the hydraulic gravitational potential of the liquid on the pump function.

The device in the form of a floating platform for the selective concentration of microflora, its antigens and DNA on the MIS with an immersion depth controlled by 2 floats relative to the liquid surface is made of specially treated foam (glued with paper and painted with oil paint) and has a niche for removable multi-channel magnetic traps with cylindrical containers filled with specific MISs held in a closed magnetic field due to permanent niobium magnets, which also fix a removable slotted water intake at the surface of the water when it is taken into the tank.

Before starting work, MIS is introduced into the cylindrical containers of the multichannel magnetic trap and the trap is placed in a niche of a floating platform, in which, due to magnetic attraction, a slot-shaped water intake is installed from below. The floating platform with the floats is placed in a tank with tap water, the liquid-draining channels are connected to the nozzles of the magnetic trap and / or slit-like intake, and air is removed with an aspirator or peristaltic pump. Using movable floats, the immersion depth of the platform is set at the level of the upper edge of the cylindrical tanks, while the slot of the intake is located in the water 2-3 mm below the water level. Connectors of the outlet branch pipe are connected to the fluid flow inducer - the aspirating channel of the microperistaltic pump (or manual aspirator), which is turned on for 8-10 seconds to check the tightness of the system and estimate its flow rate at the rate of 1.0-2.0 ml / s, with a minimum the installation level of micro- and peristaltic pumps relative to the surface of the water is 0.15-0.20 m to prevent the negative influence of the hydraulic gravitational potential of the liquid on the pump function.

Distinctive features of the claimed utility model are:

1. The presence of a floating collapsible disk container with a membrane filter on a float platform, allowing you to change the liquid level above the membrane surface

2. The ability to install the device on the surface of the reservoir due to the movable brackets using a radio-controlled floating means or telescopic pole.

3. The presence on the frame of the float platform of the disk container of 3 floats and on a floating platform with a multi-channel magnetic trap and a slotted sampler of 2 floats

4. The ability to change the immersion depth within 1-5 mm in the liquid of the floating and float platforms, respectively, due to the separate movement and fixation of each of the 2 or 3 floats for immersing the device in water to the required depth.

5. The presence of guide rods and a “lock” between the disk base and the cover of the filter device.

6. Ensuring the mechanical strength of the filter membrane due to the installation of a supporting metal mesh, resting on the edge of the cutout around the circumference of the base and the protrusion of the pipe of the liquid-discharge channel in the center of the disk base.

7. The presence in the aluminum pipe of additional side holes opening into the chamber of the disk container to increase the efficiency of fluid flow.

8. Ensuring the tightness of the chamber of the disk container due to the tight compression of its base and lid with an elastic rubber cuff, and the channels of the magnetic trap are formed by tightly inserted fragments of silicone tubes into the cylindrical niches of the trap.

9. The presence of the matrix device in the form of a disk with cutting edges for cutting filters of the required size from the membrane sheet with congruent holes for the guide rods and the "lock".

10. The presence in the kit of the handle device in the form of a U-shaped steel spacer “fork” with 2 parallel rods 70 mm long, for fixing the sealing elastic rubber cuff on the side surface of the disk container.

11. The device’s kit contains a modified container with a magnetic lid for storing sterile and / or used filters.

12. The presence of a floating platform with a removable multichannel magnetic trap with cylindrical tanks filled with specific MIS, and a removable slotted sampler installed at the surface of the water.

13. The presence of a niche in the body of the floating platform for the fixed placement of a multichannel magnetic trap with MIS and / or slotted sampling device.

14. The provision of fluid flow when using a disk container and / or a floating platform with a magnetic trap and a slit sampler is provided by an aspirator (AS USSR No. 1217067, 1985).

15. The provision of fluid flow when using a disk container and / or a floating platform with a magnetic trap and a slit sampler is provided by peristaltic pumps of a radio-controlled floating vehicle (RF Patent No. 133834. Publ. 27.10.2013 Bull. No. 30).

16. Ensuring the retention of MIS in a multichannel magnetic trap in a closed magnetic field due to permanent niobium magnets.

17. Ensuring the fixation of the slotted sampler as part of a floating platform due to permanent niobium magnets.

18. The ability to disassemble the main functional units of a disk container and a floating platform with a multi-channel magnetic trap and a slotted sampler.

General view images are shown in photos 1, 2, 3, 4, 5, 6, 7 and thumbnails of schemes of the claimed utility model and devices for providing its functions in FIG. 1,2,3,4, 5, 6, 7, 8, 9.

General view images in photo 1-7:

1. (general view). A device for the concentration of microflora on a membrane filter.

2. (general view). Matrix for cutting membrane filters of the required size from a sheet blank of the type "Vladipor Membrane";

3. (general view). Handle with a U-shaped spacer “fork” fixed to it;

4. (general view). Modified container with magnetic lid;

5. (general view). A device for the selective concentration of microflora, its antigens and DNA on MIS;

6. (general view). Multi-channel magnetic trap;

7. (general view). Slot-hole sampler.

I. In FIG. 1 (side view) shows a diagram of a floating collapsible filtering device consisting of a flat disk container 1, the body of which is formed by a disk base 2 with a “lock” rod 3 and a cover in the form of a washer 4 with 3 fixing rods 5 evenly spaced along the edge of the circle. The disk base and the cover are hermetically connected with the help of an enclosing sealing elastic rubber cuff 6 and form a chamber 7, the cover of which presses a membrane filter 8, delimiting the filter chamber from the surface of the water at a depth not 1-5 mm due to floats 9, which regulate the level of immersion of the container in water, but to prevent violation of the integrity of the membrane filter, a small metal supporting grid 10 in the form of a disk is installed under the latter, the edge of which and the center, respectively, rely on the inner protrusion of the disk base 11 and in the center on the ledge of the channel 12 with a screwing (M8 × 0.75) aluminum pipe 13 with 4 additional side openings 14 for draining fluid from the chamber of the disk container, and a fragment of the pipe branching out of the chamber chickpeas at a right angle, and serves to connect an elastic, flexible silicone tube 15 and ensure fluid flow due to a peristaltic pump 16 or aspirator 17. A movable bracket 18 is mounted on the lid of the disk container for mounting the device on the surface of the water using a telescopic pole or steering wheel radio SD3770B, the connection channel of which is configured according to the instructions of the digital proportional system iVAX-9x with built-in memory.

In FIG. 2 (top view) shows the lid 4 of the disk container, where the “lock” core 3 enters the opening of the lid 4 with 3 fixing rods 5 evenly spaced along the circumference of the circle 5. An elastic rubber sleeve 6 compresses the membrane filter 8, delimiting the filter the chamber 7 from the water surface to an adjustable depth due to the floats 9, and the movable bracket 18 is fixed by 3 rods to the cover 4.

In FIG. 3 (top view) shows the metal frame 20 of the float platform 19 with 3 lockable screws 21 and floats 9 moved along the racks 22.

In FIG. 4 (top view) shows a matrix 23 for cutting membrane filters 8 of the required size from a sheet blank (see Fig. 1) in the form of a disk, the side surface of which is circumferentially limited by a metal strip 24 with a cutting edge, and 4 metal pipes 25 s pointed edges and placed congruently to the “lock” 3 and 3 fixing rods 5 (see Fig. 1 and 2).

In FIG. 5 (view) shows a U-shaped spacer “fork” 26 with a handle 27 and 2 parallel rods 28 for installing a sealing rubber sleeve 6 around the circumference of the housing of the disk container 1 (see Fig. 1).

In FIG. 6 (side view) shows a rectangular plastic modified container 29 with a magnetic lid 32 with 2 niobium magnets 33 for storing sterile and / or used membrane filters with a lodgement 30 in the base 31. Stiffening ribs 34 are applied to the base and lid.

II. In FIG. 7 (front view) shows a floating platform 1 made of foam, glued with paper and painted with oil paint, with an adjustable immersion depth of removable functional elements - a collapsible multichannel magnetic trap 2 and a slotted sampler 3 are provided with 2 additional side floats 4 with their regulating and screws 5 fixing relative to the surface of the liquid, while the upper edge of the silicone channels in the form of cylindrical containers 6 with MIS 7 is located at the level of its surface, l 8 the sampler is installed parallel to the liquid level, niobium permanent magnets 9, which are fixed due to the magnetic field on the steel couplings 10, cover the silicone cylindrical containers and form a closed magnetic field that holds the MIS 7 and the sampler 3. In the upper part of the floating platform, a movable bracket 11 for installing the device on the surface of the water using a telescopic pole or steering wheel radio remote control SD3770B, the connection channel of which is configured according to the instructions digitally proportional iVAX-9x system with integrated memory.

In FIG. Fig. 8 (top view) shows a collapsible polychannel magnet trap 2 made of organic glass 10 mm thick in the form of 2 separable fragments 12 and 13, in the grooves of which are fixed rods 14 and silicone cylindrical containers 6 covered by steel couplings 10 with permanent niobium magnets 8, and the pipe 15 combines the channels of a magnetic trap and provides selection and concentration of antigens on the surface of the MIS 7.

In FIG. 9 (top view) shows a slotted sampler 3 made of tinned steel sheet in the form of a combined flattened parallelepiped opening in front of a slit 16 bounded by guide planes 17 set at an angle of 45 degrees relative to the horizontal surface of the water, and the opposite side was transformed into a tetrahedral prism with a nozzle 18 for fluid sampling in a 1 liter capacity.

The possibility of using a floating, collapsible device is confirmed by specific examples of its use. Example 1. The practical use of a floating, collapsible filtering device was carried out in compliance with sanitary rules (SP 1.3.1285-03 “Safety of work with microorganisms of pathogenicity (hazard) I-II”. 2003. - 118 p.). After checking the tightness of the chamber 7 of the disk filter container 1, setting its immersion level 1-2 mm from the water surface by adjusting the floats 9 on the frame platform, evaluating the liquid flow rate, a peristaltic pump 17 mounted on board was connected to the branch pipe of the discharge channel for liquid flow of a floating radio model (RF Patent No. 133834. Publ. 27.10.2013 Bull. No. 30), which with the help of radio commands and the electric drives controlled by them, extended to the surface of the reservoir, lowered the device to the water, turned on the perist The Altic pump 17 concentrated microflora on the surface of filter 8 for 10 minutes at a flow rate of 12 ml / s, which corresponded to a total water flow rate of about 7.2 liters. Then the device was disconnected, the membrane filter was removed and placed in a cylindrical storage container 24.

Example 2. It differs from example 1 in that an aspirator was connected to the branch pipe of the outlet channel through a silicone rubber tube to ensure fluid flow (A. S. USSR No. 121 17067, 1985), the device was placed on a water surface with a telescopic pole, and microflora was concentrated for 10 minutes on the surface of the membrane filter due to the muscle energy applied to the aspirator, with an average water flow rate of 8 ml / sec, which corresponded to a total water flow rate of about 4.8 liters. Then the device was disconnected, the membrane filter was removed and placed in a cylindrical storage container 24.

Example 3. It differs from example 2 in that a 1.0 liter liquid container was connected to the outlet pipe of the slit sampler, from which air was removed with an aspirator until it was filled.

Example 4. It differs from example 1 in that a microperistaltic pump was connected to the outlet pipe of a multichannel magnetic trap on a floating platform, with a fluid flow rate of 1 ml / s for 1 hour.

Example 5. It differs from example 1 in that, to the outlet pipes of the slotted water intake and the multichannel magnetic trap on a floating platform, respectively, a 1.0 liter liquid tank was connected, from which air was removed with a peristaltic pump until the tank was filled, and a microperistaltic pump with a flow rate liquid 2 ml / sec for 1 hour.

Thus, the claimed technical device “Floating collapsible device for monitoring the microflora of open water bodies and wastewater” as a utility model is practicable. Using the proposed utility model allows under various conditions and for various purposes to quickly select and concentrate microflora-containing material in containers, on membrane filters and / or magneto-immunosorbents. At the same time, microflora is not exposed to intense physical and chemical factors, and due to the minimized hydromechanical action, microorganisms retain good viability and are suitable for bacteriological studies.

Claims (16)

1. Floating collapsible device for monitoring the microflora of open water bodies and wastewater for epidemiological, microbiological and environmental monitoring of the microflora of open water bodies, characterized in that it is made in the form of a floating collapsible disk container with a membrane filter, consisting of a lid and a disk base with a protrusion of the outlet pipe through the aluminum channel pipe in the center of the base of the device, 3 floats are installed on the frame of the float platform of the disk container, and on the float s platform polikanalnoy magnetic trap with cylindrical recesses for magnets and magnoimmunosorbentov niobium Sampler slot 2 has a float which floats are movable and fixing to change the depth of immersion of the container and the liquid level above the surface of the membrane. 2. The device according to claim 1, characterized in that its floating collapsible disk container, as well as a floating platform with a multi-channel magnetic trap and a slotted sampler, are made with the possibility of installation on the surface of the reservoir due to movable brackets using a radio-controlled floating means or telescopic pole. 3. The device according to p. 2, characterized in that between the disk base and the lid of the disk container mounted guide rods and a "lock". 4. The device according to p. 3, characterized in that the strengthening of the mechanical strength of the filter membrane is ensured by installing a supporting metal mesh, resting on the edge of the cutout around the circumference of the disk base and the protrusion of the pipe of the liquid-discharge channel in the center of this base. 5. The device according to p. 4, characterized in that in the aluminum pipe to increase the efficiency of fluid flow made additional side holes that open into the chamber of the disk container. 6. The device according to claim 5, characterized in that the tightness of the chamber of the disk container is ensured by tightly compressing its base and lid with an elastic rubber cuff, and the channels of the magnetic trap are formed by tightly inserted fragments of silicone tubes into the cylindrical niches of the trap. 7. The device according to claim 6, characterized in that the disk container includes a matrix in the form of a disk with cutting edges for cutting filters of the required size from the membrane sheet with congruent holes for the guide rods and the "lock". 8. The device according to p. 7, characterized in that the handle of the disk container includes a U-shaped steel spacer “fork” with 2 parallel rods for fixing the sealing elastic rubber cuff on the side surface of the disk container. 9. The device according to p. 8, characterized in that the disk container includes a modified container with a magnetic lid and a lodgement for storing sterile and / or used filters. 10. The device according to p. 9, characterized in that it is made in the form of a floating platform with a removable multichannel collapsible magnetic trap with cylindrical containers filled with specific magnetic immunosorbents and a removable slotted sampler at the surface of the water for its gentle selection in a separate container. 11. The device according to p. 10, characterized in that a niche for a removable multichannel magnetic trap and / or a removable slotted sampling device is cut out in a floating platform. 12. The device according to p. 11, characterized in that the flow rate when using a disk container or a floating platform with a magnetic trap and a slit sampler is provided by an aspirator. 13. The device according to p. 12, characterized in that the fluid flow when using a disk container or a floating platform with a magnetic trap and a slit sampler is provided by peristaltic pumps of a radio-controlled floating facility. 14. The device according to p. 13, characterized in that the magneto-immunosorbents are held in a multichannel magnetic trap in a closed magnetic field due to permanent niobium magnets. 15. The device according to p. 14, characterized in that the slotted sampler is held in the floating platform due to the permanent niobium magnets. 16. The device according to p. 15, characterized in that it is possible to disassemble the main functional units of the disk container and the floating platform with a multi-channel magnetic trap and a slotted sampler.

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