CN109536376A - A kind of microorganisms in water original-position collecting device - Google Patents

A kind of microorganisms in water original-position collecting device Download PDF

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Publication number
CN109536376A
CN109536376A CN201811531512.5A CN201811531512A CN109536376A CN 109536376 A CN109536376 A CN 109536376A CN 201811531512 A CN201811531512 A CN 201811531512A CN 109536376 A CN109536376 A CN 109536376A
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water
filter
injection port
main body
filter membrane
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CN201811531512.5A
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CN109536376B (en
Inventor
王小祥
谢祥峰
朱洪标
陈晨
李�杰
陈朋利
闫懂懂
卓宁
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Jiangsu Environmental Protection Industry Technology Research Institute Co Ltd
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Jiangsu Environmental Protection Industry Technology Research Institute Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a kind of microorganisms in water original-position collecting devices, including main body, filter device, water absorber, drainage arrangement;Main body has water containing chamber;Main body offers injection port, and injection port is connected with water containing chamber;Filter device is provided at injection port;It is provided with water absorber in main body, provides power for entering water containing chamber by filter device for sample;Main body offers water outlet, and water outlet is connected with water containing chamber;Water outlet is provided with drainage arrangement;Filter device includes the filter base for being mounted on injection port, and filter base is matched with injection port, provides support for filter membrane;Filter device further includes filter membrane fixing clamp and hinge buckle, filter membrane fixing clamp is matched with filter base and injection port, filter membrane fixing clamp is snapped connection by hinge to be arranged in main body, and filter membrane fixing clamp can be buckled by hinge and be opened and closed with filter base and injection port;To which filter membrane is fixed in filter base and injection port by filter membrane fixing clamp.

Description

A kind of microorganisms in water original-position collecting device
Technical field
The invention belongs to biotic environment monitoring technical field more particularly to a kind of microorganisms in water original-position collecting devices.
Background technique
In recent years, the deepening continuously in each field with microbe research, survival condition, group for aquatic microorganisms The probing into have become in detection and evaluation aquatic ecosystem health status and Related Research Domain of structure and succession of herbaceous most has Imitate most direct means.Compared to detection methods such as physical chemistry, microorganism detection can more it is sensitive it is more accurate expression not Same impact factor, the influence of the substances such as different pollutants.Thus, the detection method of microorganism has become ecological evaluation and correlation is ground Study carefully the important research analysis means in field.
However, in universal microorganisms in water sampling process, technical staff first by a certain amount of water sampling, after The microorganism in water body is transferred to by Vacuum filtration device and is filtered on machine filter film, followed by the various of related microorganisms Analysis detection.Since water sampling is different from the environment that microorganism acquisition needs, often field sampling is needed to transfer to laboratory The sampling work of microorganism is not carried out.Simultaneously as microbiological analysis often needs to carry out the difference analysis between parallel sample.Cause This, the collection process of entire microorganisms in water seems especially complicated, also increases the workload of technical staff, greatly reduces The efficiency of sample acquisition, and since there are certain sample interval, microorganism may be because being in the anaerobic and aerobic stage And lead to the change of itself structure of community, and then a degree of damage and data distortion are caused to microorganism itself.
Simultaneously as the demand of different research and analysis means, the in-situ acquisition of microorganisms in water may be needed in water body Carried out under the conditions of certain depth, thus, the depth acquisition of partial region (such as reservoir, dam etc.) can increase with the depth of sampling and It becomes more complicated, and the stage is still difficult to realize now.Ratio is considered for the acquisition situation of microorganism in water in the prior art It is more single, obtained microbiological specimens can not standard the rigorous scientific analysis of progress, while can not accurately be directed to a certain depth Microorganism stability and high efficiency acquisition.
Therefore, in corresponding scientific research and aquatic monitoring, urgent need, which develops one kind, can be used for a variety of depth and easy to be easy-to-use Microorganism acquisition device in situ.
But just the acquisition of microorganisms in water in situ has the disadvantage in that at present
(1) majority, by device for fetching water and Suction filtration device, filters after acquiring water sample to right on filter membrane still by the way of manually acquiring The shallower position of water surface following depth carries out the acquisition of the samples such as microorganism in situ and inefficiency;(2) reservoir or dam etc. are perpendicular The underwater in-situ microorganism of the water body of straight latent deep big, long-term water storage work is then difficult to acquire.
Summary of the invention
Purpose: in order to overcome the deficiencies in the prior art, the present invention provides a kind of microorganisms in water in-situ acquisition dress It sets.
Technical solution: in order to solve the above technical problems, the technical solution adopted by the present invention are as follows:
A kind of microorganisms in water original-position collecting device, it is characterised in that: including main body, filter device, water absorber, draining dress It sets;
The main body has water containing chamber;
The main body offers injection port, and the injection port is connected with water containing chamber;
It is provided with filter device at the injection port, for being filtered to the sample for entering water containing chamber by injection port;
It is provided with water absorber in the main body, provides power for entering water containing chamber by filter device for sample;
The main body offers water outlet, and the water outlet is connected with water containing chamber;The water outlet is provided with drainage arrangement;
The filter device includes the filter base for being mounted on injection port, and the filter base is matched with injection port, is filter membrane Support is provided;
The filter device further includes that filter membrane fixing clamp and hinge buckle, the filter membrane fixing clamp and filter base and injection port phase Cooperation, the filter membrane fixing clamp by hinge snap connection be arranged in main body, filter membrane fixing clamp can by hinge buckle and Filter base and injection port carry out opening and closing;To which filter membrane is fixed in filter base and injection port by filter membrane fixing clamp.
Further, the microorganisms in water original-position collecting device, it is characterised in that: the filter base is to be evenly equipped with The quartzy sand core filter base in embedded hole.
Further, the microorganisms in water original-position collecting device, it is characterised in that: the injection port is round knot Structure, the filter base are round base identical with injection port internal diameter, and connection is covered with entire injection port;The filter membrane fixing clamp For cirque structure.
Further, the microorganisms in water original-position collecting device, it is characterised in that: the filter membrane fixing clamp and filtering The junction of pedestal is additionally provided with sealing ring, for being tightly connected.
Further, the microorganisms in water original-position collecting device, it is characterised in that: the water absorber includes water suction Impeller and motor, the water suction impeller are connected to motor, and provide power by motor;The motor uses waterproof machine.
Further, the microorganisms in water original-position collecting device, it is characterised in that: be equipped on the drainage arrangement Multiple drainage holes, for the water in water containing chamber to be discharged by drainage hole.
Further, the microorganisms in water original-position collecting device, it is characterised in that: it further include fixed device, it is described The top of main body is arranged in fixed device, for for the smooth water flow for collecting process and providing stable support for apparatus main body.
Further, the microorganisms in water original-position collecting device, it is characterised in that: the injection port is arranged in main body Side, the main body circumferencial direction is all provided with there are four injection port, and each injection port is provided with filter device.
Further, the microorganisms in water original-position collecting device, it is characterised in that: the filter membrane use aperture for 0.22 μm or 0.45 μm of filter membrane.
Further, the microorganisms in water original-position collecting device, it is characterised in that: the injection port is provided in round Boss structure, convenient for being cooperatively connected with filter membrane fixing clamp;
The main body uses stainless steel material.
The utility model has the advantages that a kind of microorganisms in water original-position collecting device provided by the invention, easy, stable, efficient water body Microorganism in situ acquisition device can satisfy the requirement of microorganisms in water different depth in situ and parallel sample being acquired. It has the advantage that
(1) present invention is integrated with positioning needed for microorganisms in water in-situ acquisition process, and standardization and parallel sample are adopted simultaneously The function of collection, package unit is compact-sized, easy to operate, easy to use, can mention for the collecting work of correlative study direction sample For efficient, stable technical support.
(2) the whole of filter base uses, so that the microbiological specimens later period of package unit acquisition uses and operability It increases substantially, by being used cooperatively for fixed device, so that depth of implements is unrestricted, a variety of scientific researches can be taken into account Use demand.
(3) the important composition component of water suction impeller and waterproof machine, so that package unit can be automatic, stable micro- to water body Biology carries out in-situ acquisition, saves supernumerary's work, efficient progress microbiological specimens collecting work.
Detailed description of the invention
Fig. 1 is microorganisms in water original-position collecting device structural schematic diagram in embodiment;
Fig. 2 is that microorganisms in water original-position collecting device uses schematic diagram in embodiment;
In figure: main body 1, filter device 2, water absorber 3, drainage arrangement 4, fixed device 5;Water containing chamber 11, injection port 12, water outlet Mouth 13;Filter base 21, filter membrane fixing clamp 22, hinge buckle 23.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Below Description only actually at least one exemplary embodiment be it is illustrative, never as to the present invention and its application or make Any restrictions.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creative work premise Under every other embodiment obtained, shall fall within the protection scope of the present invention.
Unless specifically stated otherwise, positioned opposite, the digital table of the component and step that otherwise illustrate in these embodiments It is not limited the scope of the invention up to formula and numerical value.Simultaneously, it should be appreciated that for ease of description, each portion shown in attached drawing The size divided not is to draw according to actual proportionate relationship.For technology, side known to person of ordinary skill in the relevant Method and equipment may be not discussed in detail, but in the appropriate case, and the technology, method and apparatus should be considered as authorizing explanation A part of book.In shown here and discussion all examples, any occurrence should be construed as merely illustratively, and Not by way of limitation.Therefore, the other examples of exemplary embodiment can have different values.It should also be noted that similar label Similar terms are indicated in following attached drawing with letter, therefore, once it is defined in a certain Xiang Yi attached drawing, then subsequent attached It does not need that it is further discussed in figure.
As shown in Figure 1, a kind of microorganisms in water original-position collecting device, including main body 1, filter device 2, water absorber 3, row Water installations 4;
The main body 1 has water containing chamber 11, the water in sample for accommodating acquisition;
The main body 1 offers injection port 12, and the injection port is connected with water containing chamber;
Filter device 2 is provided at the injection port, for being filtered to the sample for entering water containing chamber by injection port;
It is provided with water absorber 3 in the main body 1, provides power for entering water containing chamber by filter device for sample;
The main body 1 offers water outlet 13, and the water outlet is connected with water containing chamber;The water outlet is provided with draining dress Set 4.
In some embodiments, as shown in Figure 1, the filter device 2 includes the filter base 21 for being mounted on injection port 12, The filter base 21 is matched with injection port, provides support for filter membrane.
The filter device 2 further includes filter membrane fixing clamp 22 and hinge buckle 23, the filter membrane fixing clamp 22 and filtering base Seat 21 and injection port match, and the filter membrane fixing clamp 22 is arranged on the body 1 by 23 connection of hinge buckle, filter membrane fixing clamp 22 can buckle 23 by hinge carries out opening and closing with filter base and injection port;To which filter membrane be consolidated by filter membrane fixing clamp 22 It is scheduled on filter base 21 and injection port.
The filter base 21 is the carrying platform component of the filter membrane of the sample acquisition of package unit.
Further, the filter base 21 is to be evenly equipped with the quartzy sand core filter base in embedded hole.
Further, preferably, the injection port 12 is circular configuration, and the filter base 21 is and injection port The identical round base of internal diameter, connection are covered with entire injection port;The filter membrane fixing clamp 22 is cirque structure.
Further, preferably, the junction of the filter membrane fixing clamp 22 and filter base 21 is additionally provided with close Seal, for being tightly connected.
In some embodiments, as shown in Figure 1, the water absorber 3 includes water suction impeller and motor, the water suction impeller It is connected to motor, provides power by motor;The motor uses waterproof machine.Water suction impeller and waterproof machine are placed in water containing chamber Top.Enough power demands are provided for package unit membrane filtration process.
In some embodiments, it as shown in Figure 1, being equipped with multiple drainage holes on the drainage arrangement 4, is used for water containing chamber In water pass through drainage hole be discharged.
Further, preferably, to guarantee the complete of the effect and the filter membrane itself that acquire microbiological specimens using filter membrane Property, waterproof machine is Low speed electric motor, and meeting sampling apparatus via the displacement of drainage arrangement 4 is 10-20L/min.
Further, preferably, as shown in Figure 1, further including fixed device 5, the fixed device 5 is arranged in master The top of body 1, for for the smooth water flow for collecting process and providing stable support for apparatus main body.
Further, in the present embodiment, the side of main body 1 is arranged in the injection port 12, to guarantee that microbiological specimens are adopted The parallel sample demand of collection and the durability requirements of whole-sample apparatus structure, 1 circumferencial direction of main body be all provided with there are four into Sample mouth 12, each injection port 12 are provided with filter device 2.
Further, in the present embodiment, the injection port 12 is provided in round boss structure, is convenient for and filter membrane fixing clamp 22 It is cooperatively connected.
Further, it in the present embodiment, is required for convenience of the standardization etc. of subsequent Experiment on Microbiology analysis, cooperation is common Filter membrane uses aperture for 0.22 μm or 0.45 μm of filter membrane.The diameter design size of circular filter pedestal is not more than 45mm, and outer Set circular seal ring be internal diameter be 45mm and outer diameter no more than 50mm the embedding sealing ring in bottom round stainless steel structure.
In the present embodiment, it is to have connection and the dress for 11 structure of water containing chamber of catchmenting that the main body 1, which uses stainless steel material, Basis is set, remaining part is integrated in stainless steel body structure, itself has certain self weight and tension resistance slight Guarantee that package unit is stablized in certain water depth, while meeting the safe and reliable of package unit structure and sampling stability.
The components such as main portions main body 1, filter device 2, water absorber 3, drainage arrangement 4 are all made of rigid structure combination, Guarantee the stabilization and expansibility of package unit.
Fixed device 5 provides the positioning function of position in different water for package unit.The impeller that absorbs water with waterproof machine is The membrane filtration process of package unit provides power guarantee.
A kind of microorganisms in water sample original-position collecting device of the invention is applicable to water bodies of rivers and lakes and different water depths A kind of microorganism collecting work in situ of position, it is desirable to provide microbiological specimens acquisition dress in situ easy to use, convenient, efficient It sets.Stainless steel body has the round filter membrane window and hollow water containing chamber of four sides opening, provides the steady of filter membrane for collection process Fixed platform and the effect for improving sample collection.Filter base uses the sand core material of uniform pore diameter, cooperates filter membrane fixing clamp and phase The hinge answered, which buckles, provides support for microorganism-collecting filter membrane.Water suction impeller and waterproof machine are that microbe filter in situ was collected Journey provides continuous driving force.Structure of the invention is compact, easy to operate, portable easy-to-use, and microbiological specimens collecting effect is outstanding, can be outstanding The collection for completing the water body in-situs microbiological specimens such as river and lake handles work.
As shown in Fig. 2, the basic operation schematic diagram of package unit, the application method of the present apparatus are as follows: it is solid first to open filter membrane The hinge buckle 23 of clamp, use for laboratory 0.22 or 0.45 μm of filter membrane is placed in filter base 21, then by filter membrane fixing clamp 22 close, and after moving to sample, fixed device 7 are fixed in centainly stable structure, then extremely by package unit dive The required depth of water opens waterproof machine after device is stablized, and water flow is inhaled into the water containing chamber of stainless steel body 1 by device outside at this time Through closing motor after waiting for a period of time depending on water body clean-up performance, at this time filter membrane table by drainage hole discharger after in 11 Face is taken out from water by microbe filter in water to filter membrane surface, by package unit can be obtained parallel micro- of four parts of same point Biological sample.
In the description of the present application, it is to be understood that term " center ", " longitudinal direction ", " transverse direction ", "front", "rear", The orientation or positional relationship of the instructions such as "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on attached drawing institute The orientation or positional relationship shown is only the present invention and simplified description for ease of description, rather than indicates or imply signified device Or element must have a particular orientation, construct and operate for specific orientation, thus should not be understood as in present invention protection The limitation of appearance.
The above is only a preferred embodiment of the present invention, it should be pointed out that: for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (10)

1. a kind of microorganisms in water original-position collecting device, it is characterised in that: including main body, filter device, water absorber, draining dress It sets;
The main body has water containing chamber;
The main body offers injection port, and the injection port is connected with water containing chamber;
It is provided with filter device at the injection port, for being filtered to the sample for entering water containing chamber by injection port;
It is provided with water absorber in the main body, provides power for entering water containing chamber by filter device for sample;
The main body offers water outlet, and the water outlet is connected with water containing chamber;The water outlet is provided with drainage arrangement;
The filter device includes the filter base for being mounted on injection port, and the filter base is matched with injection port, is filter membrane Support is provided;
The filter device further includes that filter membrane fixing clamp and hinge buckle, the filter membrane fixing clamp and filter base and injection port phase Cooperation, the filter membrane fixing clamp by hinge snap connection be arranged in main body, filter membrane fixing clamp can by hinge buckle and Filter base and injection port carry out opening and closing;To which filter membrane is fixed in filter base and injection port by filter membrane fixing clamp.
2. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: the filter base is uniformly distributed There is the quartzy sand core filter base in embedded hole.
3. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: the injection port is round knot Structure, the filter base are round base identical with injection port internal diameter, and connection is covered with entire injection port;The filter membrane fixing clamp For cirque structure.
4. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: the filter membrane fixing clamp and mistake The junction of filter pedestal is additionally provided with sealing ring, for being tightly connected.
5. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: the water absorber includes inhaling Water impeller and motor, the water suction impeller are connected to motor, and provide power by motor;The motor uses waterproof machine.
6. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: be all provided on the drainage arrangement There are multiple drainage holes, for the water in water containing chamber to be discharged by drainage hole.
7. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: further include fixed device, institute The top that main body is arranged in fixed device is stated, for for the smooth water flow for collecting process and providing stable support for apparatus main body.
8. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: the injection port is arranged in master The side of body, the main body circumferencial direction are all provided with there are four injection port, and each injection port is provided with filter device.
9. microorganisms in water original-position collecting device according to claim 1, it is characterised in that: the filter membrane use aperture for 0.22 μm or 0.45 μm of filter membrane.
10. -9 described in any item microorganisms in water original-position collecting devices according to claim 1, it is characterised in that: the sample introduction Mouth is provided in round boss structure, is convenient for and filter membrane fixing clamp is cooperatively connected;
And/or the main body uses stainless steel material.
CN201811531512.5A 2018-12-14 2018-12-14 Water microorganism in-situ collection device Active CN109536376B (en)

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CN111172022A (en) * 2020-02-21 2020-05-19 广西贵港华堂天诺微生物科技有限公司 In-situ microorganism collection system
CN111254055A (en) * 2020-02-26 2020-06-09 中国科学院深海科学与工程研究所 Experimental method of deep sea biogeochemistry in-situ experimental device
CN111269813A (en) * 2020-02-27 2020-06-12 中国科学院城市环境研究所 Sampling device and method for acquiring micro-plastic attached microorganisms in water environment
CN113777082A (en) * 2021-08-30 2021-12-10 江苏天瑞仪器股份有限公司 Groundwater normal position detection device with more than anticipated velocity of flow

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CN107384756A (en) * 2017-08-01 2017-11-24 云南中烟工业有限责任公司 A kind of collection device and method of essence spice for cigarette contaminating microorganisms
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Publication number Priority date Publication date Assignee Title
CN111172022A (en) * 2020-02-21 2020-05-19 广西贵港华堂天诺微生物科技有限公司 In-situ microorganism collection system
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CN111269813A (en) * 2020-02-27 2020-06-12 中国科学院城市环境研究所 Sampling device and method for acquiring micro-plastic attached microorganisms in water environment
CN113777082A (en) * 2021-08-30 2021-12-10 江苏天瑞仪器股份有限公司 Groundwater normal position detection device with more than anticipated velocity of flow

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