RU161690U1 - PLANT FOR PRODUCING BIOMASS OF AEROBIC MICRO-ORGANISMS - Google Patents

Abstract

1. Installation for the production of biomass of aerobic microorganisms, characterized by the presence of a vertically oriented capacity, the presence of at least one vertically oriented capacity-satellite and the presence of connecting them in the lower parts of the first and second pipelines equipped with stimulators of the circulation of the culture fluid, the vertically oriented container is equipped with a nozzle for supplying a liquid mineral nutrient medium, a nozzle for supplying an oxygen-containing gas, a nozzle for venting the accumulated biomass of aerobic microorganisms, and also has a collar with a flange installed coaxially with the volume of the vertically oriented container, which ensures the separation of the part filled with culture fluid into the lifting and lowering channels, is equipped with an oxygen-containing gas bubbler installed in the lifting channel and connected to the oxygen-containing gas supply pipe, and at least one vertically oriented container-satellite has a nozzle for supplying a gaseous substrate and equipping placed on its rim along its vertical axis with a flanging in the upper part towards the aforementioned axis so that it separates a part of at least one vertically oriented satellite container filled with culture fluid into a lifting one equipped with a gaseous substrate bubbler and a lowering channel, this lowering channel of the tank is connected to the lifting channel of at least one vertically oriented satellite-capacity, and the lifting channel of the same capacity is connected to the lowering channel of at least EPE, odes

Description

The utility model relates to the field of microbiology and, in particular, to plants for the industrial production of bioprotein from a gaseous substrate, for example, natural gas.

The prior art installation for the cultivation of microorganisms (Invention of the Russian Federation No. 2021350, IPC S12 M 3/00, publ. 10/15/1994). It contains a chamber with devices for aeration, sterilization, draining and topping up the medium and a mechanism for moving the chamber. The chamber is an elastic container fixed on a rigid frame, divided by external clamps into communicating feeder, growth and storage compartments, while on the lower inner surface of the growth compartment there is material to immobilize cells, the accumulation compartment is made with the possibility of independent gas exchange, and rigid the frame is made with two kinks located between the compartments, installed with the possibility of changing the angle of inclination to the horizon from 0 to 45 ° and is equipped with a mechanism for its movement. In this case, parts of the frame under the storage and growth compartments are equipped with vibrators.

The disadvantage of this known installation is the low productivity of cultivating the biomass of microorganisms.

The closest in technical essence and the achieved result to the claimed installation for the production of biomass of aerobic microorganisms is an apparatus for growing microorganisms (Invention of the Russian Federation No. 2 352 626, IPC: S12M 1/02, publ. 04/20/2009 Bull. No. eleven). This unit is adopted as a prototype device. The prototype contains a container filled with culture fluid to a certain level. The tank is equipped with nozzles for supplying liquid mineral nutrient medium, air and the removal of accumulated biomass, a bubbler for supplying air connected to the air blower, a collar with a flange mounted along the axis of the tank, which serves to separate the part of the tank filled with liquid into the lifting and lowering channels. The bubbler is located in the lift channel. At the same time, the installation is additionally equipped with at least one additional tank, which is an absorber of a gaseous substrate, having a shell along the axis of the tank with a flange in the upper part toward this axis, which separates the tank filled with the culture fluid into the lifting and lowering channels. Both tanks are connected in the lower part by liquid pipelines in such a way that the lower channel of the main tank is connected to the lifting channel of the additional tank, and the lower channel of the additional tank is connected to the lifting channel of the main tank, and a liquid circulation inducer is installed between the tanks in the liquid pipe. In addition, a bubbler of a gaseous substrate is placed in the lifting channel of the additional tank. It should be noted that the flanging in the upper part of the shell of the additional capacity is made in the form of a funnel directed inside the lowering channel, symmetrical to the axis of the lowering channel, and the funnel plays the role of a gravity-type liquid-jet ejector. In this case, the shell located in the main tank has a flange inclined to the axis of the tank or to the wall of the tank to expand the lifting channel, and a chipper is installed above it. The inducer of fluid circulation in the installation of the prototype can be made in the form of a circulation pump or in the form of an axial propeller.

The disadvantage of the installation of the prototype is that it has a relatively low productivity of cultivating biomass of aerobic microorganisms.

The task to which the creation of the proposed installation is directed is the creation of industrial conditions for the production of protein mass from methane-containing substrate by high-performance plants.

The technical result expected from the use of the claimed device is to increase the productivity of cultivating biomass of aerobic microorganisms.

The claimed technical result is achieved by the fact that the installation for the production of biomass of aerobic microorganisms contains a vertically oriented container and at least one vertically oriented container-satellite, connecting them in the area of the lower parts of the first and second liquid pipelines equipped with a circulation activator culture fluid, while the vertically oriented container is equipped with a nozzle for supplying a liquid mineral nutrient medium, a nozzle for supplying an oxygen-containing gas, patr a storage compartment for collecting accumulated biomass of aerobic microorganisms, and also has a collar with flanges installed coaxially with the volume of the said vertically oriented container, which ensures the separation of the part filled with culture fluid into the lifting and lowering channels, is equipped with an oxygen-containing gas bubbler installed in the lifting channel, and connected to the nozzle for supplying oxygen-containing gas. Moreover, at least one vertically oriented container-satellite has a nozzle for supplying a gaseous substrate and is equipped with a shell arranged along its vertical axis with a flanging in the upper part to the side of the said axis so that it ensures the separation of the filled culture liquid part of at least one vertically oriented satellite-container on the lifting, equipped with a bubbler of a gaseous substrate, and the lowering channels. The lowering channel of said container is connected to the lifting channel of at least one vertically oriented satellite-container, and the lifting channel of the same capacity is connected to the lowering channel of at least one vertically oriented satellite-vessel by means of, respectively, lifting and lowering channels, while the capacity below the level of the culture fluid is equipped with a temperature sensor and a pH sensor, and above the level of the culture fluid is equipped with a carbon dioxide sensor, which information and switching connection i-nen interface with an electronic computer containing a means of visualization of information on which the computer program "Gaprin" is installed.

It is desirable that the stimulator of the circulation of the culture fluid was made in the form of a circulation pump. It is advisable that a chipper be fixed above the shell of the tank.

The claimed installation is illustrated by a number of figures:

- figure 1 schematically shows the structure of the proposed installation, equipped with one tank and one tank-satellite;

- figure 2 schematically shows the structure of the proposed installation, equipped with one tank and two tanks - satellite;

- Fig. 3 schematically shows the structure of the proposed installation, equipped with one tank and three tanks - satellite;

- figure 4 schematically shows a vertical section of the tank;

- figure 5 schematically shows a vertical section of the capacity of the satellite.

The list of positions.

1. Capacity.

1.1. The shell in the tank.

1.2. Flanging in the container.

1.3. The lowering channel in capacity.

1.4. The lifting channel in the tank.

1.5. Air bubbler.

1.6. Air collector.

1.7. Gas discharge channel for disposal.

1.8. The level of culture fluid.

1.9. Super fluid space.

2. The capacity is satellite.

2.1. The first capacity is satellite.

2.2. The second capacity is satellite.

2.3. The third capacity is satellite.

3. Safety valve.

3.1. Safety valve in the first satellite tank.

3.2. Safety valve in the second satellite tank.

3.3. Safety valve in the third satellite tank.

4. Purge gas pipeline.

4.1. The purge gas pipeline of the first satellite capacity.

4.1.1. The shutoff valve of the purge gas pipeline of the first satellite capacity.

4.2. Purge gas pipeline of the second satellite capacity.

4.2.1. The shutoff valve of the purge gas pipeline of the second satellite capacity.

4.3. Purge gas pipe of the third satellite capacity.

4.3.1. The shutoff valve of the purge gas pipeline of the third capacity is satellite.

5. Liquid pipe.

5.1. The first fluid pipe.

5.2. The second fluid pipe.

5.3. The third pipeline.

5.4. The fourth fluid pipe.

5.5. Fifth liquid pipe.

5.6. Sixth liquid pipe.

6. Device for forced fluid circulation.

7. Chipper.

8. Air duct.

9. A shell in a satellite container.

9.1. A shell in the first satellite container.

9.2. A shell in the second satellite container.

9.3. The shell in the third satellite capacity.

10. Flanging in the satellite container.

10.1. Flanging in the first satellite container.

10.2. Flanging in the second satellite container.

10.3. Flanging in the third satellite container.

11. The lifting channel in the satellite container.

11.1. The lifting channel in the first satellite container.

11.2. The lifting channel in the second satellite container.

11.3. The lifting channel in the third satellite capacity.

12. The lower channel in the satellite tank.

12.1. The lowering channel in the first satellite capacity.

12.2. The lowering channel in the second satellite container.

12.3. The lowering channel in the third satellite capacity.

13. Gravity ejector.

13.1. The first gravitational ejector.

13.2. The second gravitational ejector.

13.3. The third gravitational ejector.

14. A bubbler for sparging with carbon-containing gas.

14.1. A bubbler for sparging with carbon-containing gas in the first satellite container.

14.2. A bubbler for sparging with carbon-containing gas in the second satellite tank.

14.3. A bubbler for sparging with carbon-containing gas in the third satellite tank.

15. Gas pipeline carbon-containing gas.

15.1. Distribution gas collector of carbon-containing gas.

15.1.1. A shutoff valve on a carbon-containing gas pipeline.

15.2. Pressure regulator.

15.3. Pressure meter.

16. Heat exchanger.

16.1. Coolant supply and exhaust pipelines

16.2. Thermostat.

17. The pipeline for supplying a liquid nutrient medium.

17.1. Valve in the pipeline for supplying a liquid nutrient medium.

18. The pipeline for draining the culture fluid.

18.1. Valve in the pipe for draining the culture fluid.

19. A pipe for gas discharge from the super-liquid space for disposal.

20. Hardware-software complex.

21. The temperature sensor.

22. The pH sensor.

23. Sensor C0 ₂

24. Information and switching channels.

25. Interface.

Capacity 1 (Figure 1-Figure 4) is a part of a composite fermenter and can be made of a sheet of inert structural material (and in shape, such as a sealed vertically oriented cylinder), in particular, from a food non rusting steel 08X18H10. From a similar material can be made shell 1.1 (Figure 4) and flanging 1.2 (Figure 4), positioned in the tank 1 (Figure 1-Figure 4) symmetrically to the vertical axis of the latter. As a bubbler 1.5 (Fig. 4), a ring structure disclosed in a known source can be used (Application for invention of the Russian Federation No. 92015695, IPC С25В 9/02, publ. 02.20.1997). The bubbler 1.5 (Figure 4) is designed to saturate the culture fluid located in the tank 1 (Figure 1-Figure 4) of the inventive installation with gas in accordance with the requirements of the bioprotein production technology (in particular, with air). The air manifold 1.6 (FIG. 4) is intended to supply the aforementioned gas (air) to the bubbler 1.5 (FIG. 4). Its design may be similar to the design disclosed in a known source (Invention of the Russian Federation No. 2494287, IPC F04D 27/00, publ. 09/27/2013, Bull. No. 27). The gas discharge channel for utilization 1.7 (Figure 4) is intended for the periodic release of the tank 1 (Figure 1-Figure 4) from the gaseous waste from the production of bioprotein used as a result of fermentation. In the simplest embodiment, it is a pipeline, one of the ends of which is positioned in the super-fluid space 1.9 (Figure 4) by means of a pipe 19 (Figure 4), and the second end of which, for environmental reasons, is placed in a scrubber (not shown )

The design of the vessel-satellite, representing at least the second part of the composite fermenter, 2 (Figure 4) and the structural materials used for its manufacture can be similar to the construction and applied structural materials of the tank 1 (Figure 1-Figure 4).

Since the capacity of the satellite 2 (Figure 1-FIGS, Figure 5) is a working capacity, working under pressure, it is equipped with a safety valve 3.1 (Figure 5), which protects the capacity of the satellite 2.1 (Figure 5) from destruction with excess pressure for it in an emergency. The design of gas safety valves is well known in the art and, in particular, for the proposed installation, a known safety valve from the source can be used: invention of the Russian Federation No. 2307276, IPC F16K 17/04, publ. 09/27/2007, Bull. No. 27. The purge gas pipeline 4.1 (Fig. 5) is intended for periodic purging (mainly before and after the work on the production of bioprotein), as well as when the increased content of carbon dioxide in the satellite 2.1 tank is exceeded (Fig. 5). It is equipped with a shut-off body 4.1.1 (Figure 5), which can be structurally embodied as an ordinary valve mounted on the said purge gas pipeline 4.1 (Figure 5).

Liquid pipelines 5 (Figure 1-Figure 5) are designed to move between the containers of the claimed installation of the culture fluid and is a pipe made of a material similar in corrosion resistance to the material of the tank 1 (Fig. 1 - Fig. 4).

The device for the forced circulation of fluid 6 (Figure 4) is designed to move the culture fluid between the tank 1 (Fig. 1-Figure 4) and the first tank-satellite 2.1 (Figure 5) / or tanks - satellite 2.1, 2.2, 2.3 (Fig. 1 - Fig. 3) /. As the mentioned device, a circulation pump can be used, for example, of the Wipo-Star RS brand (http://www.allb.ru). The chipper 7 (Figure 4) is designed to reduce the level of spray formation in the tank 1 (Figure 4) and is a profiled plate fixed along the perimeter of the inner wall of the tank 1 (Figure 4) above the flange 1.2 (Figure 4) in the tank 1 ( Figure 4). The duct 8 (Figure 4) is a means of supplying air to the air manifold 1.6 (Figure 4) and can be performed as a pipe connected to an air compressor or compressor station (not shown).

The shell in the container-satellite 9.1 (Figure 5) is designed to form a lowering 12.1 (Figure 5) in it and can be made in the form of a vertically oriented pipe or duct separating the zone of the lifting channel 11.1 (Figure 5) from the zone of the lowering channel 12.1 (Figure 5).

The first gravitational ejector 1.3 (Fig. 5) is a funnel-shaped connection of the shell 9.1 (Fig. 5) with a second fluid pipe 5.2 (Fig. 5) having a smaller flow area, which facilitates the use of Earth's gravity to accelerate the movement of the culture fluid in the second fluid pipe 5.2 (Figure 5) of the first capacity satellite 2.1 (Figure 5).

The design and operation of the bubbler for bubbling the culture fluid with carbon-containing gas 14.1 (Figure 5) are similar to the bubbler for air and, accordingly, are structurally similar in embodiment. The exception is that through a bubbler 14.1 (Figure 5), natural gas (carbon-containing gas) is supplied to the satellite-2.1 vessel (Figure 5), and not air (which is the source of oxygen). A bubbler for sparging the culture fluid with carbon-containing gas 14.1 (Figure 5) is connected to a carbon-containing gas pipeline 15 (Figure 5), equipped with a gas distribution manifold 15.1 (Figure 5), a shut-off body 15.1.1 (Figure 5), and also the maintenance system in the capacitance-satellite 2.1 (Figure 5) as part of a pressure sensor 15.3 (Figure 5) and a pressure regulator 15.2 (Figure 5), a predetermined pressure of the carbon-containing (natural) gas. Systems for maintaining in a closed volume a predetermined pressure of a gaseous medium supplied to this closed volume by means of a pressure regulator receiving information from a pressure sensor are known (see, for example, USSR AS No. 1505253, IPC G05D 16/06, publ. July 10, 2006 Prop. Bull. No. 19).

The heat exchanger 16 (Figure 4) is designed to control (including to maintain) the temperature of the culture fluid passing through the zone of the lowering channel 1.3 (Figure 4) in the tank 1 (Figure 4). It can be implemented as a tubular heat exchanger communicating via pipelines for supplying and removing coolant 16.1 (Figure 4) with a thermostat 16.2 (Figure 4), at which a specific temperature is set (for the specific type of aerobic microorganisms used)).

The functional purpose in the structure of the claimed installation of the pipeline for supplying a liquid nutrient medium 17 (Figure 5) and the pipeline for draining the culture fluid 18 (Figure 4) follows from the names of these structural elements of the installation and, obviously, does not require additional explanation. These pipelines are equipped with corresponding valves (shutoff valves) 17.1 (Fig. 1) and 18.1 (Fig. 1).

The pipe 19 (Figure 4) is used to discharge the exhaust gases accumulating in the super-fluid space 1.9 (Figure 4) of the container 1 (Figure 4) for disposal through the corresponding channel 1.7 (Figure 4), as mentioned previously, which is pipeline.

The hardware and software complex 20 used in the installation (Figure 4) is based on an IBM compatible computer equipped with a visualization tool (display) with the GAPRIN program installed on it (State Registration Certificate No. 20156188884 of 08/19/2015). It is connected to a temperature sensor 21 (FIG. 4) fixed inside the tank 1 (FIG. 4) / for example, from DANFOSS, model MVT 5260, http://www.danfoss.com/, to a pH sensor 22 (FIG. 4 ) / for example, the company Apa- CONT LE, http://www.rusavtomatic.ru/ and the CO2 sensor, 23 (Figure 4) / for example, the company WEXON, model VAISALA GMT220, http://www.wexon.ru/ through the corresponding information and switching channels (for example, in the form of wired communication lines) 24 (Figure 4) via interface 25 (Figure 4). The operation of the claimed device is illustrated by examples.

Example No. 1.

In the present example, the installation was used in the configuration shown in Fig. 1, namely, consisting of a container 1 (Fig. 1) with a volume of 50 m ³ and a first satellite-2.1 capacity (Fig. 1) with a volume of 20 m ³ connected between a first 5.1 (FIG. 1) and a second 5.2 (FIG. 1) liquid pipelines, the first 5.1 (FIG. 1) liquid piping being equipped with a pipe for draining the culture fluid 18 (FIG. 1), on which the valve 18.1 is mounted (FIG. 1), and the second 5.2 (Figure 1) the liquid pipe is equipped with a pipe wire for supplying a liquid nutrient medium 17 (Figure 1), also contains shut-off valves in de valve 17.1 (FIG. 1).

In order to prepare the claimed installation for operation, water is pumped through it with open valves 17.1 (Fig. 1) and 18.1 (Fig. 1) (water is supplied through valve 18.1 (Fig. 1) through a pipe for draining the culture fluid 18 (Fig. 1), and water outlets, respectively, through a pipeline for supplying a liquid nutrient medium 17 (Figure 1)) in a volume of 210 m ^{3 of} water heated to 49 ° C (GOST R 51232-98). During this pumping of water, the temperature of the heat exchanger 16 (Figure 4) by means of the thermostat 16.2 (Figure 4) and pipelines for supplying and discharging the liquid coolant 16.1 (Figure 4) are also set at 49 ° C. After the pumping of water is completed, it is drained from the installation and, having closed the valve 18.1 (Figure 1), the liquid mineral medium is pumped into the installation through the supply pipe of the liquid nutrient medium 17 (Figure 1) with the valve 17.1 open (Figure 1), filling approximately 75% as capacity 1 (Figure 1), and the first capacity satellite 2.1 (Figure 1). Then, through the same pipeline, a culture fluid containing the strain of the bacterium Methylococcus capsula-rs VSB-874 in the amount of 0.5% weight per 1 liter of liquid nutrient medium is fed into the installation. After filling the tank 1 (Fig. 1) to 80% (i.e., to the level 1.8 (Fig. 4 and Fig. 5), the valve 17.1 (Fig. 1 and Fig. 4) is closed. forced circulation of fluid 6 (Figure 4). Due to the effect of the latter, the culture fluid through the first fluid pipe 5.1 (Figure 4) enters the lifting channel 11.1 (Figure 5) of the first satellite-2.1 capacity (Figure 5). in the first satellite-container 10.1 (Figure 5), the culture fluid rushes into the shell in the first satellite-vessel 9.1 (Figure 5), whence due to the influence of gravitational forces and, of course, the first gravitational ejector 13.1 (Figure 5), enters the second fluid pipe 5.2 (Figure 5), then moving from it into the lifting channel 1.4 (Figure 4) in the tank 1 (Figure 4), filling the latter to level 1.8 ( In this case, the chipper 7 (Fig. 4) prevents intensive spray formation by directing the flow of the culture fluid into the lowering channel in the reservoir 1.3 (Fig. 4), where the culture fluid again comes into contact with the heat exchanger 16 (Fig. 4). ) Thus, the temperature of the culture fluid is stabilized to a value of 49 ° C. The operation of the installation for the forced circulation of the culture fluid 6 (Figure 4) and the action of gravity again causes the culture fluid to move from the lowering channel in the container 1.3 (Figure 4) into the first container-satellite 2.1 (Figure 1) and further along the route already described above. A continuous supply of air through the duct 8 (Figure 4) (excess pressure is 1.3 atm.) Leads to the filling of the first air manifold 1.6 with air (Figure 4) and the movement of air into the first bubbler 1.5 (Figure 4) ) as a result of which the culture fluid is saturated with oxygen. Not soluble in the culture fluid, the air enters the super-fluid space 1.9 (Figure 4) and then is discharged through the pipe 19 (Figure 4) through a pipe 1.7 (Figure 4) for disposal. In the first satellite-vessel 2.1 (Figure 5), the natural gas entering through the gas pipeline 15 of carbon-containing gas 15 (Figure 5) is reduced to a pressure of 1.3 atm. through the joint operation of the pressure regulator 15.2 (Fig. 5) and the pressure sensor 15.3 (Fig. 5) and through an open shut-off element in the gas pipeline 15.1.1 (Fig. 5) it fills the gas distribution manifold 15.1 (Fig. 5), and from it, it is injected into the culture fluid by means of a bubbler for sparging with carbon-containing gas in the first satellite-container 14.1 (Figure 5). Natural gas that is not dissolved in the culture fluid, together with metabolic products, accumulates in the super-fluid space 1.9 (Figure 5) and periodically (about 1 time per hour) through the shut-off element of the purge gas pipeline of the first satellite 4.1.1 tank (Figure 5) ) is displayed by the operator through the purge gas pipeline of the first satellite-4.1 capacity (Figure 5) also for disposal. In the event of an emergency operation of the installation (for example, due to the failure of the pressure regulator 15.2 (Fig. 5) and the appearance of excess natural gas pressure exceeding the established emergency value of 1.8 atm.) In the super-fluid space 1.9 (Fig. 5), the safety valve is actuated in the first satellite 3.1 vessel (Figure 5), which relieves excess pressure from the first satellite 2.1 vessel (Figure 5). Thanks to the sensors 21-23 (Fig. 4), information on the parameters of the fermentation process is received through the information-switching channels 24 (Fig. 4) through the interface 25 (Fig. 4) of the hardware-software complex 20 (Fig. 4) and is shown on the display (not shown) after processing by the Haprin computer program. Both instantaneous values of parameters taken by sensors 21-23 (Figure 4) can be visualized, as well as data subjected to analytical processing (in particular, trends in the parameters, their modules, etc.), which allows the operator to control - to the task of the claimed installation, to promptly (in particular, when the indicator deviates by more than 0.5%) respond to these events, I will correct the fermentation mode.

After 10 hours of fermentation, open the valve on the pipe for draining the culture fluid 18.1 (Figure 5) and enriched with bioprotein culture fluid through the pipe for draining the culture fluid 18 (Figure 5) is transferred to further processing, in particular for separation.

At the same time, the withdrawn amount (by volume of withdrawal) of the enriched culture fluid is replaced by the same amount (by volume) of liquid mineral nutrient medium containing the strain of the bacterium Methylococcus capsulatus VSB-874 in an amount of 0.5% weight per 1 liter of liquid mineral nutrient medium, by opening the valve in the pipeline for supplying the liquid nutrient medium 17.1 (Fig. 5) and, accordingly, supplying the aforementioned liquid nutrient medium to the second liquid pipe 5.3 (Fig. 5) of the inventive installation.

Such ten-hour fermentation cycles were carried out as part of the implementation of this example. 5. Comparison with the prototype device of the bioprotein productivity by both units (proposed and the prototype) are shown in Table No. 1.

The design and initial structural parameters of the prototype device were completely identical to the claimed device, including the presence of a hardware-software complex 20 (Figure 4) and sensors 21-23 (Figure 4) to it, however, the operator when using it in the prototype device mode did not respond to the readings displayed on the display of the hardware-software complex 20 (Figure 4) and, accordingly, did not adjust the fermentation mode during all 50 hours of operation of the prototype device.

Table number 1

Object name Fermentation time, hour The amount of bioprotein (the content of crude protein is 76%), kg Variations in temperature in the culture fluid, ° C Fluctuations in pH in culture fluid Vibrations
С0 ₂ gas phase in the super-fluid space,% vol. Prototype Device fifty 8 232 42-49 4,5-5,5 11-50 Claimed device fifty 10 815 43.5-44.5 4.8-5.1 11-14

As follows from Table No. 1, the claimed device provides more bioprotein, which indicates the achievement of the claimed technical result in the form of an increase in the productivity of cultivating biomass of aerobic microorganisms.

Example No. 2.

In the present example, the installation used in the configuration shown in Figure 2, namely, consisting of a tank 1 (Figure 2) with a volume of 50 m ³ , as well as a first satellite-2.1 vessel (Figure 2) with a volume of 15 m ³ and the second satellite capacity 2.2 (FIG. 2) with a volume of 15 m ³ interconnected by the first 5.1 (FIG. 2), the second 5.2 (FIG. 2), the third 5.3 (FIG. 2) and the fourth 5.4 (FIG. 2) fluid pipelines, and the first 5.1 (Figure 2) fluid pipe is equipped with a pipe for draining the culture fluid 18 (Figure 2), on which the valve 18.1 is mounted (Figure 2), and the second 5.2 (Figure 2) liquid pipe system is equipped with a pipe a wire for supplying a liquid nutrient medium 17 (Figure 2), and also contains valves in the form of a valve 17.1 (Figure 2). To prepare the installation for work through it with open valves 17.1 (Fig. 2) and 18.1 (Fig. 2) pumped (water is supplied through valve 18.1 (Fig. 2) through a pipeline for draining the culture fluid 18 (Fig. 2), and water outlets, respectively, through a pipeline for supplying a liquid nutrient medium 17 (Fig.Z)) 230 m ³ of water heated to 49 ° С (GOST R 51232-98). During this pumping of water, the temperature of the heat exchanger 16 (Fig. 4) by means of the thermostat 16.2 (Fig. 4) and pipelines for supplying and discharging the liquid coolant 16.1 (Fig. 4) are also set at 49 ° C. After completing the pumping of water, it is drained from the installation and, having closed the valve 18.1 (Figure 2), the liquid medium is pumped into the installation through the pipeline supplying liquid nutrient medium 17 (Figure 2) with the valve 17.1 open (Figure 2), filling approximately 75% as capacity 1 (Figure 2), so the first capacity is satellite 2.1 (Figure 2) and the second capacity is satellite 2.2 (Figure 2). Then, the culture fluid containing the strain of the bacterium Methylococcus capsulatus VSB-874 in the amount of 0.5% weight per 1 liter of liquid nutrient medium is fed into the unit through the same pipeline. After filling the tank 1 (Figure 2) to 80% (i.e., to the level 1.8 (Figure 4 and Figure 5), the valve 17.1 (Figure 2 and Figure 4) is closed. forced circulation of fluid 6 (Figure 4). Due to the effect of the latter, the culture fluid through the first fluid conduit 5.1 (Figure 4) enters the lift channel 11.1 (Figure 5) of the first capacity satellite 2.1 (Figure 5) / and, respectively into the same channel of the second satellite capacity 2.2 (Figure 2) .From it, bending around the flanging in the first satellite capacity 10.1 (Figure 5) / and, accordingly, in the second satellite capacity 2.2 (Figure 2) /, culture n I liquid rushes into the casing in the first satellite container 9.1 (Figure 5) / and, accordingly, in the second satellite container 2.2 (Figure 2) /, whence due to the influence of gravitational forces and the operation of the first gravitational ejector 13.1 (Figure 5) / and, accordingly, the second gravitational ejector 13.2 (not shown) in the second satellite container 2.2 (Figure 2) / enters the second liquid pipe 5.2 (Figure 5), then moving from it into the lifting channel 1.4 (Figure 4) in the tank 1 (Figure 4), filling the latter to the level 1.8 (Figure 4). In this case, the chipper 7 (Fig. 4) prevents intensive droplet formation by directing the flow of the culture fluid into the lowering channel in the container 1.3 (Fig. 4), where the culture fluid again comes into contact with the heat exchanger 16 (Fig. 4). Thus, the temperature of the culture fluid is stabilized to a value of 49 ° C. The operation of the installation for forced circulation of the culture fluid 6 (Figure 4) and the action of gravity again causes the culture fluid to move from the lowering channel in the container 1.3 (Figure 4) to the first 2.1 and second 2.2 of the tank-satgelite (Figure 2) and further along the route already described above. A continuous supply of air through the duct 8 (Figure 4) (overpressure is 1.3 atm.) Leads to the filling of the first air manifold 1.6 with air (Figure 4) and the movement of air into the first bubbler 1.5 (Figure 4) ) as a result of which there is a saturation of the culture fluid with oxygen. The air, which did not dissolve in the culture fluid, enters the super-fluid space 1.9 (Figure 4) and is subsequently discharged through the pipe 19 (Figure 4) through a pipeline 1.7 (Figure 4) for disposal. In the first 2.1 and second 2.2 tanks-satellites (Figure 2), the natural gas entering through the gas pipeline of carbon-containing gas 15 (Figure 5) is reduced to a pressure of 1.3 atm. through the joint operation of the pressure regulator 15.2 (Figure 5) and the pressure sensor 15.3 (Figure 5) (also available in the design and the second tank-satellite 2.2 (Figure 2) and through an open shut-off element on the gas pipeline 15.1.1 (Figure 5) ) fills the distribution gas manifold 15.1 (Fig. 5), and is injected from it into the culture fluid by means of a bubbler for sparging with carbon-containing gas in the first satellite container 14.1 (Fig. 5) / and, accordingly, in the second satellite container 14.2 (not shown). Natural gas not dissolved in the culture fluid -was in the super-fluid space 1.9 (Figure 5) of the first and second satellite containers 2.1 and 2.2 (Figure 2) and periodically (about 1 time per hour) through the shut-off element of the purge gas pipeline of the first satellite-vessel 4.1.1 (Figure .5) (and 4.2.1 in the second container-satellite 2.2 (Figure 2)) is output by the operator through the purge gas pipeline of the first container-satellite 4.1 (Figure 5) / and through the purge gas pipeline of the second container-satellite 4.2 (not shown ) / also for disposal. In the event of an emergency operation of the installation (for example, due to the failure of the pressure regulator 15.2 (Figure 5) and the appearance of excessive pressure of natural gas) exceeding the established emergency value of 1.8 atm.) In the super-fluid space 1.9 (Figure 5 ) of any capacity-satellite 2.1-2.2 (Figure 2), the safety valve is actuated, which relieves the overpressure from the emergency capacity-satellite-satellite (Figure 2).

Thanks to the sensors 21-23 (Figure 4), information on the parameters of the fermentation process is received through the information and switching channels 24 (Figure 4) through the interface 25 (Figure 4) of the hardware-software complex 20 (Figure 4) and is displayed (not shown) after processing the computer program "Gaprin". Both instantaneous values of parameters taken by sensors 21-23 (Fig. 4) and data subjected to analytical processing (in particular, trends in parameters, their modules, etc.) can be visualized, which allows the operator , which controls the operation of the claimed installation, to promptly (in particular, when the indicator deviates by more than 0.5%) respond to these events, I adjust the fermentation mode. After 10 hours of fermentation, open the valve on the pipe for draining the culture fluid 18.1 (Figure 2) and the bioprotein-rich culture fluid through the pipe for draining the culture fluid 18 (Figure 2) is transferred to further processing, in particular , for separation. At the same time, the withdrawn amount (by volume of withdrawal) of the enriched culture fluid is compensated by the same amount (by volume) of liquid nutrient medium by opening the valve on the pipeline for supplying the liquid nutrient medium 17.1 (Figure 2) and, accordingly, - the task of the above-mentioned liquid nutrient medium in the second fluid line 5.3 (Figure 2) of the claimed installation. Such ten-hour fermentation cycles were performed as part of the execution of this example. 5. Comparative data on the bioprotein performance with both plants (proposed and prototype) are shown in Table No. 2 with the prototype device. The design and initial structural parameters of the prototype device were completely identical device, including the presence of a hardware-software complex 20 (Figure 4) and sensors 21-23 (Figure 4) to it, however, the operator, when using it in the prototype device mode, does not react al on indications displayed on the display hardware and software system 20 (Figure 4) and, respectively, the adjustment mode is not produced during the fermentation of 50 hours of operation of the prototype device.

Table number 2

Object name Fermentation time, hour The amount of bioprotein (the content of crude protein is 76%), kg Variations in temperature in the culture fluid, ° C Fluctuations in pH in culture fluid Oscillations C0 ₂
the gas phase in the liquid space,% vol. Prototype Device fifty 8,238 42-49 4,5-5,5 11-50 Claimed device fifty 10 815 43.5-44.5 4.8-5.1 11-14

As follows from Table No. 2, the claimed device provides more bioprotein, which indicates the achievement of the claimed technical result in the form of an increase in the productivity of cultivating biomass of aerobic microorganisms.

Example No. 3.

In the third example, the installation was used in the configuration shown in Fig.Z, namely, consisting of a tank 1 (Fig.Z) with a volume of 50 m ³ , as well as the first 2.1, second 2.2 and third 2.3 satellite containers (Fig.Z) volume of 10 m ³ each, connected to each other by the first 5.1 (Fig.Z), second 5.2 (Fig.Z), third 5.3 (Fig. 3), fourth 5.4 (Fig.Z), fifth 5.5 (Fig.Z) ) and the sixth 5.6 (Fig. 3) fluid pipelines, the first 5.1 (Fig. 3), the third 5.3 (Fig. 3) and fifth 5.5 (Fig. 3) the fluid pipelines are parallel to each other and equipped with a pipe for draining the culture fluid 18 (Fig. H) on which with mounted valve 18.1 (Fig. H), and the second 5.2 (Fig. H), the fourth 5.4 (Fig. H) and the sixth 5.6 (Fig. H) liquid pipelines are also parallelized between them and equipped with a pipeline for supplying a liquid nutrient medium 17 ( Fig.Z), containing stop valves in the form of a valve 17.1 (Fig.Z). To prepare the unit for operation through it with open valves 17.1 (Fig. 3) and 18.1 (Fig. 3), water is pumped with volume (water is supplied through valve 18.1 (Fig. 3) through a pipe to drain the culture fluid 18 (Fig. H), and water outlets, respectively, through a pipeline for supplying a liquid nutrient medium 17 (Fig.Z)) 230 m ^{3 of} water heated to 49 ° C (GOST R 51232-98). During this pumping of water, the temperature of the heat exchanger 16 (Fig. 4) by means of the thermostat 16.2 (Fig. 4) and pipelines for supplying and discharging the liquid coolant 16.1 (Fig. 4) are also set at 49 ° C. After completion of the pumping of water, it is drained from the installation and, having closed the valve 18.1 (Fig. 3), the liquid medium is pumped into the installation through the liquid supply pipe 17 (Fig. 3) with the valve 17.1 open (Fig. 3), filling approximately 75% as capacity 1 (FIG. 3), as well as all three satellite capacities 2.1, 2.2, 2.3 (FIG. 3). Then, the culture fluid containing the strain of the bacterium Methylococcus capsulatus VSB-874 in the amount of 0.5% weight per 1 liter of liquid nutrient medium is fed into the unit through the same pipeline. After filling the tank 1 (Fig. 3) to 80% (ie, to level 1.8 (Fig. 4), the valve 17.1 (Fig. 3 and Fig. 4) is closed. The device for forced circulation of the fluid 6 is started up. (Fig. 4). Due to the effect of the latter, the culture fluid through the first fluid pipe 5.1 (Fig. 4) enters the lifting channel 11.1 (Fig. 5) of the first satellite-2.1 vessel (Fig. 5) / and, accordingly, the same channel of the second and third satellite containers 2.2 and 2.3 (Fig. 3) /. From it, enveloping the flanging in the first satellite satellite 10.1 (Figure 5) / and, respectively, in the second and third satellite containers max 2.2 and 2.3 (Fig. H) /, the culture fluid is mounted in the casing in the first satellite container 9.1 (Fig. 5) / and, respectively, in the casing of the second and third satellite containers 2.2 and 2.3 (Fig. C) /, whence under the influence of gravitational forces and due to the operation of the first gravitational ejector 13.1 (Fig. 5) / and, accordingly, the second and third gravitational ejectors 13.2 and 13.3 (not shown) in the second and third satellite containers 2.2 and 2.3 (Fig. .3) / enters the second liquid pipe 5.2 (Figure 5), then moving from it to the lifting channel 1.4 (Figure 4) in the tank 1 (Figure 4), filling latter to the level of 1.8 (Figure 4). In this case, the chipper 7 (Figure 4) prevents intensive droplet formation by directing the flow of the culture fluid into the lowering channel in the reservoir 1.3 (Figure 4), where the culture fluid again comes into contact with the heat exchanger 16 (Figure 4). Thus, stabilization of the temperature of the culture fluid to a value of 49 ° C. The operation of the installation for forced circulation of the culture fluid 6 (Figure 4) and the action of gravity again causes the culture fluid to move from the lowering channel in the container 1.3 (Figure 4) into the first 2.1, second 2.2 and third satellite tanks (Fig .3) and further along the route already described above. Continuous air supply through the duct 8 (Figure 4) (overpressure is 1.3 atm.) Leads to the filling of the first air manifold 1.6 with air (Figure 4) and the movement of air into the first bubbler 1.5 (Figure 4) due to what is the saturation of the culture fluid with oxygen. Not soluble in the culture fluid, the air enters the super-fluid space 1.9 (Figure 4) and then is discharged through the patrol side 19 (Figure 4) through the pipeline 1.7 (Figure 4) for disposal. In the first 2.1, second 2.2 and third 2.3 satellite containers (Fig. 3), the natural gas entering through the gas-containing carbon-containing gas pipeline 15 (Fig. 5) is reduced to a pressure of 1.3 atm. through the joint operation of the pressure regulator 15.2 (Figure 5) and the pressure sensor 15.3 (Figure 5) (also available in the design of the second 2.2 and third 2.3 capacity-satellites (Fig.Z) and through an open shut-off element on the gas pipeline 15.1.1 (Fig .5) it fills 15.1 (Fig. 5) into the gas distribution manifold and is injected from it into the culture fluid by means of a bubbler for sparging with carbon-containing gas in the first satellite container 14.1 (Fig. 5) / and, accordingly, in the second 2.2 and the third 2.3 satellite containers 14.2 and 14.3 (not shown) /. Not dissolved in the culture Natural gas accumulates in the superfluid space 1.9 (Fig. 5) of the first 2.1, second 2.2 and third 2.3 satellite containers (Fig. H) and periodically (about 1 time per hour) through the shut-off element of the purge gas pipeline of the first tank - satellite 4.1.1 (Fig. 5) /4.2.1 in the second satellite container 2.2 (Fig. 3) and 4.3.1 of the third 2.3 satellite container (Fig. 3) / is displayed by the operator through the purge gas pipeline of the first satellite-4.1 container (Figure 5) / and through the purge gas pipelines of the second 2.2 and third 2.3 of the satellite containers 4.2 and 4.3 (not shown) / also for disposal. In the event of an emergency operation of the installation (for example, due to the failure of the pressure regulator 15.2 (Figure 5) and the appearance in the tank-satellite 2.1 (Figure 5) of excess natural gas pressure in excess of 1.8 atm. ) in the super-fluid space 1.9 (Fig. 5) of any satellite-2.1-1.3 container (Fig. 3), a safety valve is activated that relieves excess pressure from the emergency satellite-tank (Fig. 3).

Thanks to the sensors 21-23 (Figure 4), information on the parameters of the fermentation process is received through the information and switching channels 24 (Figure 4) through the interface 25 (Figure 4) of the hardware-software complex 20 (Figure 4) and is displayed (not shown) after processing the computer program "Gaprin". Both instantaneous values of parameters taken by sensors 21-23 (Fig. 4) and data subjected to analytical processing (in particular, trends in parameters, their maximum or minimum modules, etc.) can be visualized, which It allows the operator, controlling the operation of the claimed installation, to quickly (in particular, when the indicator deviates by more than 0.5%) respond to these events, and adjust the fermentation mode.

After 10 hours of fermentation, open the valve on the pipe for draining the culture fluid 18.1 (Fig.Z) and enriched with bioprotein culture fluid through the pipe for draining the culture fluid 18 (Fig.Z) is transferred to further processing, in particular for separation. At the same time, the withdrawn amount (by volume of withdrawal) of the enriched culture fluid is compensated by the same amount (by volume) of liquid nutrient medium by opening the valve on the pipeline for supplying the liquid nutrient medium 17.1 (Fig. 3) and, accordingly, by feeding the above-mentioned liquid nutrient medium in the second liquid pipe 5.3 (Fig.Z) of the claimed installation. Such ten-hour fermentation cycles were performed as part of the execution of this example. 5. Comparative data on the bioprotein performance with both plants (proposed by the installation and the prototype device) are shown in Table No. 3 with the prototype device. The design and initial structural parameters of the prototype device during delivery tests were completely identical to the claimed device, including the presence of a hardware-software complex 20 (Figure 4) and sensors 21-23 (Figure 4) to it, however, the operator at and using it in the prototype device mode did not respond to the readings displayed on the display of the hardware-software complex 20 (Figure 4) and, accordingly, did not adjust the fermentation mode during all 50 hours of operation of the prototype device.

Table number 3

Object name Fermentation time, hour The amount of bioprotein (the content of crude protein is 76%), kg Variations in temperature in the culture fluid, ° C Fluctuations in pH in culture fluid Vibrations
С0 ₂ gas phase in the super-fluid space,% vol. Prototype Device fifty 8 229 42-49 4,5-5,5 11-50 Claimed device fifty 11 2513 43.5-44.5 4.8-5.1 11-14

As follows from Table No. 3, the claimed device provides a large amount of bioprotein, which indicates the achievement of the claimed technical result in the form of an increase in the productivity of cultivating biomass of aerobic microorganisms.

To create the proposed installation, materials, components and mechanisms known from the prior art can be used, which allows us to conclude that the proposal meets the eligibility criterion of the utility model “industrial applicability”.

Claims (3)

1. Installation for the production of biomass of aerobic microorganisms, characterized by the presence of a vertically oriented capacity, the presence of at least one vertically oriented capacity-satellite and the presence of connecting them in the lower parts of the first and second pipelines equipped with stimulators of the circulation of the culture fluid, the vertically oriented container is equipped with a nozzle for supplying a liquid mineral nutrient medium, a nozzle for supplying an oxygen-containing gas, a nozzle for venting the accumulated biomass of aerobic microorganisms, and also has a collar with a flange installed coaxially with the volume of the vertically oriented container, which ensures the separation of the part filled with culture fluid into the lifting and lowering channels, is equipped with an oxygen-containing gas bubbler installed in the lifting channel and connected to the oxygen-containing gas supply pipe, and at least one vertically oriented container-satellite has a nozzle for supplying a gaseous substrate and equipping placed on its rim along its vertical axis with a flanging in the upper part towards the aforementioned axis so that it separates a part of at least one vertically oriented satellite container filled with culture fluid into a lifting one equipped with a gaseous substrate bubbler and a lowering channel, this lowering channel of the tank is connected to the lifting channel of at least one vertically oriented satellite-capacity, and the lifting channel of the same capacity is connected to the lowering channel of at least Here, one vertically oriented satellite container through respectively the lifting and lowering channels, while the tank below the level of the culture fluid is equipped with a temperature sensor and a pH sensor, and above the level of the culture fluid is equipped with a carbon dioxide sensor, which are connected by an interface to an electronic computer containing means visualization of information on which the Haprin computer program is installed. 2. Installation for the production of biomass of aerobic microorganisms according to claim 1, characterized in that the circulation fluid of the culture fluid is made in the form of a circulation pump. 3. Installation for the production of biomass of aerobic microorganisms according to claim 1, characterized in that a chipper is fixed above the shell of a vertically oriented container.

Images