RO98166B1 - Laboratory method for determining anti-native dna antibodies - Google Patents

Laboratory method for determining anti-native dna antibodies

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Publication number
RO98166B1
RO98166B1 RO129893A RO12989387A RO98166B1 RO 98166 B1 RO98166 B1 RO 98166B1 RO 129893 A RO129893 A RO 129893A RO 12989387 A RO12989387 A RO 12989387A RO 98166 B1 RO98166 B1 RO 98166B1
Authority
RO
Romania
Prior art keywords
native dna
dna antibodies
laboratory method
determining anti
staining
Prior art date
Application number
RO129893A
Other languages
Romanian (ro)
Inventor
Horea Rus
Ioan Florin Niculescu
Daniela Malide
Anca Cristea
Rodica Rahaian
Maria Petcovici
Anca Diana Maican
Vasilica Olaru
Maria Rus
Dorohoi Ionescu
Original Assignee
Institutul Cantacuzino
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institutul Cantacuzino filed Critical Institutul Cantacuzino
Priority to RO129893A priority Critical patent/RO98166B1/en
Publication of RO98166B1 publication Critical patent/RO98166B1/en

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Abstract

Metoda conform inventiei consta în aceea ca lamele cu hemoflagelatul Crithidia luciliae, care au fost incubate cu serul de testat, se trateaza la temperatura camerei, timp de 1 h, cu 20...50 miu 1 conjugat de IgG de iepure antigamaglobulina umana-peroxidata, care este diluat 1/20 în tampon fosfat salin, urmând apoi colorarea, evidentierea kinetoplastului si interpretarea în mod cunoscut.The method according to the invention consists in that the lamellae with Crithidia luciliae hemophlagetate, which were incubated with the test serum, were treated at room temperature for 1 hour with 20-50 ml of human-peroxidized antigamaglobulin rabbit IgG conjugate , which is diluted 1/20 in saline phosphate buffer, followed by staining, kinetoplast staining and interpretation in the known manner.

RO129893A 1987-09-29 1987-09-29 Laboratory method for determining anti-native dna antibodies RO98166B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RO129893A RO98166B1 (en) 1987-09-29 1987-09-29 Laboratory method for determining anti-native dna antibodies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RO129893A RO98166B1 (en) 1987-09-29 1987-09-29 Laboratory method for determining anti-native dna antibodies

Publications (1)

Publication Number Publication Date
RO98166B1 true RO98166B1 (en) 1990-01-30

Family

ID=40906278

Family Applications (1)

Application Number Title Priority Date Filing Date
RO129893A RO98166B1 (en) 1987-09-29 1987-09-29 Laboratory method for determining anti-native dna antibodies

Country Status (1)

Country Link
RO (1) RO98166B1 (en)

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