RO87006B1 - Process for obtaining ovine antivariolic vaccine - Google Patents
Process for obtaining ovine antivariolic vaccineInfo
- Publication number
- RO87006B1 RO87006B1 RO110713A RO11071383A RO87006B1 RO 87006 B1 RO87006 B1 RO 87006B1 RO 110713 A RO110713 A RO 110713A RO 11071383 A RO11071383 A RO 11071383A RO 87006 B1 RO87006 B1 RO 87006B1
- Authority
- RO
- Romania
- Prior art keywords
- ovine
- vaccine
- obtaining
- antivariolic
- centrifugation
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 229960005486 vaccine Drugs 0.000 title abstract 2
- 235000019687 Lamb Nutrition 0.000 abstract 2
- 238000005119 centrifugation Methods 0.000 abstract 2
- 230000002381 testicular Effects 0.000 abstract 2
- 230000003612 virological effect Effects 0.000 abstract 2
- 238000004113 cell culture Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 238000010166 immunofluorescence Methods 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 238000007920 subcutaneous administration Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 238000010257 thawing Methods 0.000 abstract 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Inventia se refera la un procedeu de obtinere a vaccinului antivariolic ovin, prin cultivare pe culturi de celule primare sau linii de celule renale si testiculare de miel, congelarea si decongelarea rapida a masei virale de 2...3 ori, centrifugarea suspensiei virale la 3000 tur/min, titrarea virusului centrifugat prin metoda "in vitro" sau prin imunofluorescenta indirecta pe culturi de celule primare renale si testiculare de miel si în final inocularea subcutan în cantitate de 1000...5000 DICP50, în vol de 0,5 ml.The invention relates to a process for obtaining the ovine antivarial vaccine by cultivation on primary cell cultures or renal and testicular lamb cell lines, fast freezing and defrosting of the viral mass 2 or 3 times, centrifugation of the viral suspension at 3000 per minute, centrifugation by in vitro method or indirect immunofluorescence on primary kidney and lamb testicular cultures and finally subcutaneous inoculation of 1000 to 5000 DICP50 in 0.5 ml volumes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO110713A RO87006B1 (en) | 1983-04-20 | 1983-04-20 | Process for obtaining ovine antivariolic vaccine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO110713A RO87006B1 (en) | 1983-04-20 | 1983-04-20 | Process for obtaining ovine antivariolic vaccine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO87006A2 RO87006A2 (en) | 1985-08-30 |
| RO87006B1 true RO87006B1 (en) | 1985-08-31 |
Family
ID=20112956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO110713A RO87006B1 (en) | 1983-04-20 | 1983-04-20 | Process for obtaining ovine antivariolic vaccine |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO87006B1 (en) |
-
1983
- 1983-04-20 RO RO110713A patent/RO87006B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO87006A2 (en) | 1985-08-30 |
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