RO122523B1 - Lotion for hair care - Google Patents

Abstract

The invention relates to a lotion for hair care, which is characterized in that it consists of 13.05 g panthenole, 300g of 87% concentration alcohol, 0.275 g menthol, 15 g camomile extract, 15 g isopropyl alcohol, 30 g nettle extract, 30 g birch extract, 10 g germabene, 30 g glycerine, 0.55 bronopole, 9.9 lipoprotein complex and 546.125 g distilled water.

Description

The invention relates to a hair care lotion, which has lately become a rather serious problem.

At the moment there are a number of shampoos, conditioners and hair care lotions, but these remedies are mostly made of synthetic chemicals, which have a lot of side effects, not showing their praised effects.

The problem solved by the invention is to establish the components and their association ratio leading to an increase in the bioavailability of the lotion.

The invention relates to a hair care lotion containing 13.05 g panthenol, 300 g 87% alcohol, 0.275 menthol, 15 g chamomile extract, 15 g isopropyl alcohol, 30 g nettle extract, 30 g birch extract, 10 g germaben, 30 g glycerin, 0.55 g bronopol, 9.9 lipoprotein complex and 546.125 g distilled water.

The advantage of the invention is that it achieves a deep hair restoration effect, due to the use of natural substances.

This lotion is composed only of natural substances, stimulating hair growth, helping to remove dandruff by strengthening the hair, restoring its natural color.

These special qualities of the lotion are due to the biologically active substance, which is a lipoprotein complex, extracted from the beetle (Mellallenta mețlallenta L.) at a certain stage of its development.

This complex contains 97.12 g / 100 g dry matter, which includes the following: 50.09 g / 100 mg protein, 20.92 mg / 100 g lipids and 4.03 mg / 100 g carbohydrates. Proteins (nitrogen x 6.25) of which: total nitrogen 12.11, amino acids 4.38, nitrogen precipitating with ATC1.67 (in%).

The lipoprotein complex, after burning leaves 1.53% of total ash; 0.0012 ash insoluble in HCl110%.

Ca -182 mg / 100 g

Mg - 87 mg / 100 g

P-23 mg / 100 g.

Among the amino acids, the following predominate:

Amino acid composition of the preparation "Mm"

AMINO mg / 1000 mg Glutamic acid 79,745 Phenylalanine 58,912 Aspartic acid 52,078 lysine 44,479 leucine 36,371 Glycine 34,883 arginine 36,127 alanine 31,169 proline 31,073 Threonine 27,976 isoleucine 25,971 valine 23,404 Serin 22,374 Pyrolysine 15,934

the most obvious being glutamic acid, phenylalanine, aspartic acid, lysine and leucine.

An embodiment of the invention is given below.

The hair care lotion is prepared in an equipped and sterile laboratory; the steps to obtain the lotion are as follows:

RO 122523 Β1

- collection of May beetles by means of special traps, 1

- washing them with water and physiological solution until the removal of impurities;

- crushing the beetles with special grinders until obtaining a homogeneous paste 3 of gray color;

- filtration of the suspension until the removal of particulars greater than 90 mk;

- centrifuging the suspension until a homogeneous paste is obtained;

- freeze-drying or spraying the paste until a gray-brown powder is obtained;

- obtaining the lotion from the mentioned components;

- packing the lotion in 150 ml bottles; from the total amount of water - 556.175 g is separated by 50 g of water to dissolve the bronchopoly and the lipoprotein complex; in the remaining water the depanthenol is dissolved, and separately the menthol in alcohol, after which the solutions are mixed, 11 adding chamomile extract with isopropyl alcohol, nettle and birch extract, glycerin, germaben.13

Thus, the following ingredients are used to obtain the lotion:

- distilled water - 546.125 g;

- panthenol -13.05 g;

- 87% alcohol - 300 g, 17

- menthol - 0.275 g;

- chamomile extract -15 g; 19

- isopropyl alcohol -15g;

- nettle extract - 30 g, 21

- birch extract - 30 g;

- germaben -10 g; 23

- glycerin - 30 g;

- bronopol - 0.55 g, 25

- lipoprotein complex - ie 9.9 g.

The given composition is stirred with a stirrer for 5-7 min, after which it is allowed to rinse for 15-20 min and packed in sterile vials of 150 ml e.

The biologically active substance (lipoprotein complex) is obtained as follows: on May 29, when the May beetles appear, they are collected at night, with the help of light traps in areas with maximum density. The beetles are kept in plastic vessels, 300-400, 31 for 12-14 h, to remove the feces, then wash 3-4 times in clean spring water, then in saline (at 101 water distilled add 9 g of pure table salt), where 33 hold for 15-20 min. After that, the beetles are washed with distilled water for 15-20 minutes.

All beetles are ground with a special mortar at 5000 rpm for 15-16 minutes, 35 until a gray paste is formed. The obtained mass is filtered through a grinder with 200 holes per cm ² . After filtering the suspension, a light gray mass is obtained, which is centrifuged at a speed of 37 5000 rpm for 15-18 min. The sediment is separated and lyophilized or atomized, obtaining a light brown powder, which is preserved in special vessels at temperatures of 39 15-20'C.

This lotion, due to the biologically active substance it contains, stops hair loss, strengthens it, strengthens its root, stimulates the pigment and restores its suppleness, regenerating it.

The lipids are separated after centrifugation by means of the Soxhlet apparatus, after which the solvent 43 is removed by means of a rotovapor. The procedure to be performed is as follows:

1. the biological material is placed in the Soxhlet extractor packed in 45 paper cartridges covered with cotton wool or filter paper sachets;

2. the flask is filled with an adequate volume of organic solvent (petroleum ether, xylene, 47 benzene, acetone, etc.), placed on a water or sand bath with the temperature set to a value close to the boiling point of the solvent; 49

3. extraction is continued for 10-12 hours;

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4. the lipid extract in the organic solvent is evaporated in a rotary evaporator and the lipids are obtained in concentrated form. If the flask has been brought to constant weight, the percentage of lipids in the raw material, whose moisture has previously been determined, can be calculated.

Thus, with the hair care lotion begins a new era in cosmetology, the era of the use of biologically active substances, extracted from insects.

The lipids it contains include fats with unsaturated acids, very different from animal and vegetable ones. This lotion, in addition to its extraordinary properties, also gives volume to the hair, without fattening it for 5-6 days, so there is no need to wash the hair at short intervals.

Acute toxicity of substances H _L -1, H _L -2, IM-2, IM _SPP , administered enteral

Dosage H _l -1 H _l -2 IM-2 IM _SPP No animals Death No animals Death No animals Death No animals Death 750 mg / kg male 6 - 6 6 - 6 - 750 mg / kg females 6 - 6 6 - 6 - 1000 mg / kg male 6 - 6 - 6 - 6 - 1000 mg / kg females 6 - 6 - 6 - 6 - 1500 mg / kg male 6 - 6 - 6 - 6 - 1500 mg / kg females 6 - 6 - 6 - 6 - 2000 mg / kg male 6 - 6 - 6 - 6 - 2000 mg / kg females 6 - 6 - 6 6 -

Acute toxicity to parenteral administration of the substances was studied in mice and white rats, obtained from the same source. Acute toxicity testing at intraperitoneal injection of suspensions of the investigated substances was initiated with doses that constitute about 50% of those used enteral. After administration of the established doses, the animals were monitored until changes in their mortality and behavior disappeared. During the observation, the behavior of the animals, motor activity, coordination of movements, reaction to physiological excitations (light, noise), respiratory activity, condition of the eyes, skin and mycoses were recorded. Also, the time of occurrence of intoxication phenomena and the time of animal death (relaxation and interruption of convulsions, respiratory and cardiac arrest) were recorded. Visually, the clinical picture of intoxication was determined. The results were statistically processed using the Kurber method and the statistical method of probabilities after V.B. Prozorovschii.

Thus, when administered the substance H _L -1 in mice in both males and females, LD ₁₀₀ was 500 mg / kg body weight (although the initial dose that caused the death of 100% animals was 750 mg / kg) . Subsequently, the following successive doses of 250,100 and 25 mg / kg were taken. The obtained results allowed us to find that LD ₀ was 25 mg / kg body weight. In animals, there is a decrease in motor activity, with a reduced reaction, then increasingly weak, to exogenous stimuli (light, noise, etc.) with the development of a terminal state (gasping) and the death of animals for 30-60 min , at doses of 750 and 500 mg / kg or a few hours (2-4 h), at doses of 250 and 100 mg / kg, which survived after a period of hypodynamics, and reduced response to exogenous stimuli; motor activity and reactivity to stimulants were restored. The animals returned to their original state within 24 hours.

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Acute toxicity of H _C 1, H _L -2, IM-2, IM _SPP , 25 administered intraperitoneally to mice

Dosage H _l -1 H _l -2 IM-2 IM _SPP 27 29 No animals Death No animals Death No animals Death No animals Death 1000 mg / kg male - - 5 5 - - - - 31 1000 mg / kg females - 5 5 5 5 - - 750 mg / kg male 10 10 10 9 5 5 5 5 33 750 mg / kg females 5 5 5 1 5 5 5 5 500 mg / kg females 5 5 5 - 5 4 5 3 35 250 mg / kg male 7 6 5 - 5 3 - - 250mg / kg females 5 4 5 - 5 2 5 2 37 100 mg / kg male 5 3 - - 5 - 5 - 100 mg / kg females 5 2 - - 5 - 5 - 39 wmg / kg male 5 - - - - - - - 25mg / kg females 5 - - - - - - - 41

When H _L -2 was administered to both male and female mice, LD ₁₀₀ found 43 was 1000 mg / kg body weight. Subsequently, the following successive doses were made: 750,500 and 250 mg / kg. The obtained results allowed us to find that LD ₀ was 250 mg / kg 45

RO 122523 Β1 body weight for males and 500 mg / kg for females. In the animals in the experiment there is a decrease in motor activity, with a reduced reaction, then weaker, to 3 exogenous stimuli (light, noise), with the development of a terminal state (gasping) and the death of animals for 30- 60 min at doses of 1000 mg / kg or a few hours (2-4 h) at doses of 5,750 and 500 mg / kg in males, while in females, at a dose of 750 mg / kg, only one animal died . Seizures were found only occasionally. In mice, at doses of 750 and 500 mg / kg, which survived after a period of hypodynamics and reduced response to exogenous stimuli, motor activity and reactivity to stimulants were restored. The animals returned to baseline 9 within 24 h.

When administered the substances IM-2 and IM _SPP in both male and female mice, LD ₁₀₀ 11 was 750 mg / kg body weight. Subsequently, the following successive doses of 500, 250, and 100 mg / kg were made. The obtained results allowed us to find that LD ₀ was 13 100 mg / kg body weight for males and females. In animals, there is a decrease in motor activity, with a reduced reaction, then weaker, to exogenous stimuli (light, 15 noise), with the development of a terminal state (gasping) and the death of animals for 3060 min at doses of 750 mg / kg or a few hours (2-4 h), at doses of 250 and 500 mg / kg. 17 Seizures were found only occasionally. In mice, at doses of 2500 and 500 mg / kg, which survived after a period of hypodynamics and reduced reaction to exogenous stimuli, motor activity and reactivity to stimulants was restored. The animals returned to their original state within 24 hours.

Acute toxicity of substances H _L -1, H _L -2, IM-2, IM _SPP ,

2. 3 administered intraperitoneally to rats 25 Dosage h _l - 1 H _l - 2 IM-2 IM _S IPP 27 No animals Death No animals Death No animals Death No animals Death 1000 mg / kg females - - 5 5 - 5 - - 29 750 mg / kg male - - 5 5 - 5 - - 750 mg / kg females - - 6 1 - 5 5 5 31 600 mg / kg females - - 6 - - - - - 500 mg / kg male 5 5 6 3 6 6 5 5 33 500mg / kg females 6 6 5 - 6 6 5 5 250 mg / kg male 5 5 5 - 6 6 5 5 35 100 mg / kg females 6 6 - - 6 3 6 4 100 mg / kg male - - - - 6 2 6 3 37 100 mg / kg females 6 5 - - 6 - 6 - 150 mg / kg male - - - - - - - 39 150 mg / kg females - - - - - - 6 2 75 mg / kg male - - - - - - - - 41 75 mg / kg females 5 4 - - - - - - 50 mg / kg male 6 6 - - 5 - - - 43 50 mg / kg females - - - - - - - - 25 mg / kg male 6 2 - - - - * - 45 25 mg / kg females 6 2 - - - - - - 5 mg / kg male 5 - - - - - - - 47 5 mg / kg females 5 - - - - - - -

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Determination of toxicity of biologically active substances (SBA) extracted from insects1

SBA (H _l - 1, H _l -2, IM-2, IM _spp ) are obtained from insect tissues at certain stages of development, using specially developed technologies.3

Technological scheme of mass reproduction of insects:

- selection of insect eggs;

- preparation of nutritive environments;

- laying eggs on nutrient media;

- sanitation of cassettes with larvae;

- collecting insects at certain stages of development;

- extraction of SBA from insect tissues according to special technologies;

- preservation of SBA at low temperatures.11

This study presents the toxic doses (maximum, average and minimum) of the researched substances, as well as the character and level of pathological changes of some sensitive organs or organ systems13. The reversibility of these changes over time was also determined, as well as the local tolerance of the researched substances.15

Determination of acute toxicity of SBA Nr. 1 (H _L -1), no. 2 (H _L -2), no. 3 (IM-2) and No. 4 (IM _SPP )

The research is carried out according to the “methodological recommendations for the study of the general toxicity17 of pharmacological substances approved by the State Pharmacological Committee of the Russian Ministry of Health from 29.12.97.19

Determination of acute toxicity of SBA by single enteral and intraperitoneal administration of given substances21

Materials and methods:

The study included 430 white rats (216 females and 214 males) aged 3-23 months, with a body weight between 150-240 g, as well as 190 mice (100 males and 90 females) with a body weight of 16-26 g. Age and body weight were selected according to the requirements 25 indicated in the given recommendations.

The animals are obtained from the same source and kept for 3 days in the living conditions of the laboratory (standard plastic boxes) for acclimatization. Living conditions and nutrition corresponded to health requirements and food norms. 29 water mains have been established for water use. The animals did not receive food 12 hours before and during the experiment. In the morning of the experimental day, 31 rats and mice were weighed and divided into homogeneous groups, 5-6 males and 5-6 females, according to body weight. 33

The investigated substances are insoluble in water in which they form a uniform suspension.

They were diluted in constant volumes of 0.9% saline (3-5 ml for each rat when administered orally and 1 -3 ml for the maximum permissible amount of fluid for enteral and intraperitoneal administration (as instructed). 37 route of administration-enteral (in rats) and intraperitoneal (in rats and mice).

Thus, the hair care lotion obtained from biologically active substances 39 extracted from insects differs from all hair care lotions in that it stimulates hair growth, removes dandruff, regenerates hair, restores its natural color and gives it suppleness and firmness. .

Claims (2)

Claims 1. Hair care lotion characterized in that it contains 13.05 g panthenol, 300 g 87% alcohol, 0.275 g menthol, 15 g chamomile extract, 15 g isopropyl alcohol, 47 30 g nettle extract, 30 g extract of birch, 10 g germaben, 30 g glycerin, 0.55 g bronopol, 9.9 g lipoprotein complex and 546.125 g distilled water. 49 RO 122523 Β1 1 Hair care lotion according to claim 1, characterized in that the lipoprotein complex consists of dry matter 97.12 g / 100 g, containing 3 50.09 g / 100 mg protein, 20.92 mg / 100 g lipids and 4.03 mg / 100 g and is extracted when the amount of unsaturated fatty acids is maximum.