RO100893B1 - Obtention method of a pectolytic enzyme - Google Patents
Obtention method of a pectolytic enzymeInfo
- Publication number
- RO100893B1 RO100893B1 RO134263A RO13426388A RO100893B1 RO 100893 B1 RO100893 B1 RO 100893B1 RO 134263 A RO134263 A RO 134263A RO 13426388 A RO13426388 A RO 13426388A RO 100893 B1 RO100893 B1 RO 100893B1
- Authority
- RO
- Romania
- Prior art keywords
- pectolytic enzyme
- obtention
- obtention method
- icpvilf
- upg
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Procedeul conform inventiei consta în aceea ca microorganismul Aspergillus niger, înregistrat în colectia ICPVILF la nr. 3, se cultuva pe un mediu de cultura ce contine tescovina de mere, tarate de grau, fosfat disodic, oxalat de amoniu si clorura de sodiu, la temperatura de 30.....32 degree C, timp de 120 h si la o rotatie la interval de 10 min, la pH= 4,5 biomasa obtinuta se ameateca cu apa sterila si se agita timp de 2 h la 200 rot/min, amestecul obtinut se filtreaza, se centrifugheaza si se concentreaza pana la un continut de 400 UPG/ml.The process according to the invention consists in that the microorganism Aspergillus niger, registered in the ICPVILF collection at no. 3 is grown on a culture medium containing apple marc, wheat bran, disodium phosphate, ammonium oxalate and sodium chloride at a temperature of 30 ... 32 ° C for 120 hours and a rotation at 10 min at pH = 4.5, the biomass obtained was sterilized with water and shaken for 2 h at 200 rpm, the resulting mixture was filtered, centrifuged and concentrated to 400 UPG / ml.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RO134263A RO100893B1 (en) | 1988-06-30 | 1988-06-30 | Obtention method of a pectolytic enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RO134263A RO100893B1 (en) | 1988-06-30 | 1988-06-30 | Obtention method of a pectolytic enzyme |
Publications (2)
Publication Number | Publication Date |
---|---|
RO100893A2 RO100893A2 (en) | 1991-03-14 |
RO100893B1 true RO100893B1 (en) | 1991-03-30 |
Family
ID=20122038
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RO134263A RO100893B1 (en) | 1988-06-30 | 1988-06-30 | Obtention method of a pectolytic enzyme |
Country Status (1)
Country | Link |
---|---|
RO (1) | RO100893B1 (en) |
-
1988
- 1988-06-30 RO RO134263A patent/RO100893B1/en unknown
Also Published As
Publication number | Publication date |
---|---|
RO100893A2 (en) | 1991-03-14 |
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