RO100303B1 - Reactivi pentru tipizarea serologica a tulpinilor de pseudomonas aeruginosa si procedeu de obtinere - Google Patents
Reactivi pentru tipizarea serologica a tulpinilor de pseudomonas aeruginosa si procedeu de obtinereInfo
- Publication number
- RO100303B1 RO100303B1 RO131652A RO13165288A RO100303B1 RO 100303 B1 RO100303 B1 RO 100303B1 RO 131652 A RO131652 A RO 131652A RO 13165288 A RO13165288 A RO 13165288A RO 100303 B1 RO100303 B1 RO 100303B1
- Authority
- RO
- Romania
- Prior art keywords
- serum
- monovalent
- reactivs
- strums
- standardisation
- Prior art date
Links
- 210000002966 serum Anatomy 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 5
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 241000191940 Staphylococcus Species 0.000 abstract 2
- 239000003086 colorant Substances 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Reactivii, conform inventiei, sunt constituiti din 1 volum suspensie de stafilococ 10% obtinuta pe mediu bulion-cord si cu un continut de 0,5 % formaldehida si fie 0,1 volum ser aglutinat monovalent corespunzator fiecarui derotip I-XXII din schema Meitert-Meitert pentru obtinerea reactivului monovalent, fie cu 0,1 volum ser polivalent care este obtinut din amestecul de seruri monovalente I, II, IV, VI, XVI sau III, V, VIII, XIII sau VII, XI, XIV, XV sau IX, X, XII, XVII sau XVIII, XIX, XX, XXI, XXII precum si un colorant ales dintre Cl 9633923, Cl 22120, Cl 16230 sau Cl 42095. Procedeul de obtinere a reactivilor consta în aceea ca suspensia de stafilococ obtinuta în fermentator, tratata imediat cu formol si apoi spalata si justata cu TFS la concentratia de 10% (g/v) se pune în contact cu serul monovalent sau polivalent la 22 degree C timp de 2 h, cu agitare intermitenta si apoi se coloreaza la temperatura camerei cu solutie 1,5% din colorantul ales adaugat în cantitate de 1 : 0,00015, dupa care se mentine 24 h la 4 degree C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO131652A RO100303A2 (ro) | 1988-01-06 | 1988-01-06 | Reactivi pentru tipizarea serologica a tulpinilor de pseudomonas aeruginosa si procedeu de obtinere |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO131652A RO100303A2 (ro) | 1988-01-06 | 1988-01-06 | Reactivi pentru tipizarea serologica a tulpinilor de pseudomonas aeruginosa si procedeu de obtinere |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO100303B1 true RO100303B1 (ro) | 1990-11-30 |
| RO100303A2 RO100303A2 (ro) | 1991-05-02 |
Family
ID=20120938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO131652A RO100303A2 (ro) | 1988-01-06 | 1988-01-06 | Reactivi pentru tipizarea serologica a tulpinilor de pseudomonas aeruginosa si procedeu de obtinere |
Country Status (1)
| Country | Link |
|---|---|
| RO (1) | RO100303A2 (ro) |
-
1988
- 1988-01-06 RO RO131652A patent/RO100303A2/ro unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RO100303A2 (ro) | 1991-05-02 |
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