PT2032698E - Avaliação sistemática de relações entre sequência e actividade utilizando bibliotecas de avaliação de locais em relação a múltiplas propriedades de engenharia - Google Patents

Avaliação sistemática de relações entre sequência e actividade utilizando bibliotecas de avaliação de locais em relação a múltiplas propriedades de engenharia Download PDF

Info

Publication number
PT2032698E
PT2032698E PT07796357T PT07796357T PT2032698E PT 2032698 E PT2032698 E PT 2032698E PT 07796357 T PT07796357 T PT 07796357T PT 07796357 T PT07796357 T PT 07796357T PT 2032698 E PT2032698 E PT 2032698E
Authority
PT
Portugal
Prior art keywords
evaluation
sequence
activity relationships
multiple properties
libraries
Prior art date
Application number
PT07796357T
Other languages
English (en)
Inventor
Wolfgang Aehle
David A Estell
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38659837&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PT2032698(E) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of PT2032698E publication Critical patent/PT2032698E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
PT07796357T 2006-06-23 2007-06-22 Avaliação sistemática de relações entre sequência e actividade utilizando bibliotecas de avaliação de locais em relação a múltiplas propriedades de engenharia PT2032698E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81620206P 2006-06-23 2006-06-23
US93331207P 2007-06-06 2007-06-06

Publications (1)

Publication Number Publication Date
PT2032698E true PT2032698E (pt) 2012-09-24

Family

ID=38659837

Family Applications (1)

Application Number Title Priority Date Filing Date
PT07796357T PT2032698E (pt) 2006-06-23 2007-06-22 Avaliação sistemática de relações entre sequência e actividade utilizando bibliotecas de avaliação de locais em relação a múltiplas propriedades de engenharia

Country Status (12)

Country Link
US (3) US20080004186A1 (pt)
EP (1) EP2032698B1 (pt)
JP (1) JP5785686B2 (pt)
KR (1) KR101486087B1 (pt)
CN (1) CN101473036B (pt)
CA (1) CA2654269C (pt)
DK (1) DK2032698T3 (pt)
ES (1) ES2388753T3 (pt)
HK (1) HK1128934A1 (pt)
PL (1) PL2032698T3 (pt)
PT (1) PT2032698E (pt)
WO (1) WO2008002472A2 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2159279A3 (en) 2001-05-15 2010-05-12 Novozymes A/S Alpha-amylase variant with altered properties
US7985569B2 (en) * 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
MX2009013084A (es) * 2007-06-06 2010-01-18 Danisco Us Inc Genencor Div Metodos para mejorar las propiedades de la proteina.
EP2279251A2 (en) 2008-04-23 2011-02-02 Danisco US Inc. Isoprene synthase variants for improved microbial production of isoprene
MX2010013123A (es) * 2008-06-06 2011-01-14 Danisco Inc Alfa-amilasas variantes de bacillus subtilis y metodos de uso de las mismas.
AU2009256280B2 (en) * 2008-06-06 2013-03-07 Danisco Us Inc. Geobacillus stearothermophilus alpha-amylase (AmyS) variants with improved properties
EP2414514B1 (en) * 2009-04-01 2015-05-27 Danisco US Inc. Compositions and methods comprising alpha-amylase variants with altered properties
CN102656265A (zh) 2009-04-23 2012-09-05 丹尼斯科美国公司 异戊二烯合酶的三维结构及其用于产生变体的用途
MX2012006616A (es) * 2009-12-09 2012-06-21 Procter & Gamble Productos para el cuidado de las telas y el hogar.
CN103052704A (zh) 2010-07-22 2013-04-17 荷兰联合利华有限公司 用于提高清洁的鼠李糖脂和酶的组合物
CN103443271A (zh) * 2010-10-27 2013-12-11 丹尼斯科美国公司 用于增加异戊二烯产量的异戊二烯合酶变体
WO2012071458A1 (en) 2010-11-22 2012-05-31 Array Biopharma Inc. Heterocyclic inhibitors of beta - secretase for the treatment of neurodegenerative diseases
MX2013009177A (es) * 2011-02-16 2013-08-29 Novozymes As Composiciones detergentes que comprenden metaloproteasas de m7 o m35.
US9434932B2 (en) 2011-06-30 2016-09-06 Novozymes A/S Alpha-amylase variants
DE102011118027A1 (de) 2011-09-12 2013-03-14 Henkel Ag & Co. Kgaa Verfahren zur Anpassung eines Hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente
WO2013110766A1 (en) 2012-01-26 2013-08-01 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
JP6067409B2 (ja) 2012-04-10 2017-01-25 花王株式会社 アルカリプロテアーゼの溶解性向上方法
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
WO2013189972A2 (en) 2012-06-20 2013-12-27 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
CN104968211A (zh) 2013-02-06 2015-10-07 诺维信公司 具有蛋白酶活性的多肽在动物饲料中的用途

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5700676A (en) * 1984-05-29 1997-12-23 Genencor International Inc. Modified subtilisins having amino acid alterations
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5322770A (en) * 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
EP1921148B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
US6355255B1 (en) * 1998-12-07 2002-03-12 Regents Of The University Of Minnesota Streptococcal C5a peptidase vaccine
MA25044A1 (fr) 1997-10-23 2000-10-01 Procter & Gamble Compositions de lavage contenant des variants de proteases multisubstituees.
AU1225099A (en) * 1997-11-21 1999-06-15 Novo Nordisk A/S Protease variants and compositions
DE69837559T3 (de) 1997-12-24 2011-07-21 Genencor International, Inc., Calif. Verfahren zur Bestimmung der Waschleistung eines Enzyms.
US6376450B1 (en) * 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
US6933140B1 (en) * 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
US6582914B1 (en) * 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
DE10129187A1 (de) * 2001-06-19 2003-01-02 Studiengesellschaft Kohle Mbh Optimierung von synthetischen Katalysatoren durch gerichtete Evolution
DK1523553T3 (da) 2002-01-16 2010-04-19 Genencor Int Multi-substituerede proteasevarianter
WO2005005654A1 (en) * 2003-07-11 2005-01-20 Novozymes A/S Method of screening for improved specific activity of enzymes
WO2005040408A1 (de) * 2003-10-22 2005-05-06 Nascacell Ip Gmbh Verfahren zur identifizierung von enzymen mit gewünschten eigenschaften durch verankerung der reaktionsprodukte auf der oberfläche enzympräsentierender organismen
US7985569B2 (en) * 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
CA2546451A1 (en) 2003-11-19 2005-06-09 Genencor International, Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
ATE500322T1 (de) * 2003-12-03 2011-03-15 Genencor Int Perhydrolase
US20060040327A1 (en) * 2004-08-18 2006-02-23 Terry Amiss Methods of screening proteins
DK2390321T3 (en) * 2005-10-12 2015-02-23 Procter & Gamble The use and manufacture of a storage stable neutral metalloprotease

Also Published As

Publication number Publication date
WO2008002472A3 (en) 2008-03-13
CA2654269C (en) 2015-09-22
HK1128934A1 (en) 2009-11-13
US20140187440A1 (en) 2014-07-03
WO2008002472A2 (en) 2008-01-03
US8648015B2 (en) 2014-02-11
CN101473036B (zh) 2012-12-12
CA2654269A1 (en) 2008-12-03
DK2032698T3 (da) 2012-09-03
EP2032698B1 (en) 2012-06-27
ES2388753T3 (es) 2012-10-18
KR101486087B1 (ko) 2015-01-26
US20080004186A1 (en) 2008-01-03
JP2009540862A (ja) 2009-11-26
PL2032698T3 (pl) 2012-11-30
KR20090023408A (ko) 2009-03-04
EP2032698A2 (en) 2009-03-11
JP5785686B2 (ja) 2015-09-30
US20110082048A1 (en) 2011-04-07
CN101473036A (zh) 2009-07-01

Similar Documents

Publication Publication Date Title
HK1128934A1 (en) Systematic evaluation of sequence and activity relationships using site evaluation libraries for engineering multiple properties
IL251673A0 (en) Clinical applications of neurophysiological pattern processing
PL1989231T3 (pl) Terapia kombinatoryczna z udziałem antagonistów alfa5beta1
ATE509113T1 (de) Verbesserte grg23-epsp-synthasen: zusammensetzungen und verfahren zu ihrer verwendung
BRPI0719195A8 (pt) inibidores de quinase, método de modular uma atividade quinase e composição farmacêutica
PL2848610T3 (pl) Inhibitory aktywności kinazy
PT2397142T (pt) Utilizações de inibidores da dpp iv
HK1137758A1 (en) Inhibitors of akt activity akt
ATE531720T1 (de) Aza-benzofuranylverbindungen und anwendungsverfahren dafür
EP2023718A4 (en) CREATINLIGATE COMPOUNDS AND APPLICATION METHOD THEREFOR
PL1999136T3 (pl) Peptydomimetyczne inhibitory PSMA, związki je zawierające i sposoby ich zastosowania
BRPI0716300A2 (pt) Artigo e mistura
EP2010310A4 (en) SUBSTANCE MIXING SYSTEMS AND DEVICES AND METHODS FOR THEIR MANUFACTURE
EP2046413A4 (en) MEDICAL SYSTEMS AND APPLICATION METHOD
IL197060A0 (en) Aza-benzothiophenyl compounds and methods of use
EP2038228A4 (en) DECONTAMINATION FLUIDS AND METHODS OF USE THEREOF
ZA200808017B (en) Tetrahydropyridothienopyrimidine compounds and methods of use thereof
EP2058666A4 (en) MICROPLATE SEPARATOR AND MICROPLATE SEPARATION METHOD
HK1137756A1 (en) Substituted dihydropyrazolones and use thereof as hif-prolyl-4 -hydroxylase inhibitors hif--4-
EP2120951A4 (en) INHIBITORS OF AKT ACTIVITY
GB0614365D0 (en) Pharmaceutical compositions and their use
EP2101575A4 (en) ACE2 ACTIVATOR COMPOUNDS AND METHOD OF USE THEREOF
ZA200900896B (en) Aza-benzofuranyl compounds and methods of use
GB0712245D0 (en) Combinatorial libraries and related methods
DK2099460T3 (da) 6-alkoxyalkylestradiolderivater og fremgangsmåder til anvendelse