PT109359A - PORTABLE DEVICE, METHOD FOR READING AUTHENTICATION LABELS AND ITS USES. - Google Patents

PORTABLE DEVICE, METHOD FOR READING AUTHENTICATION LABELS AND ITS USES. Download PDF

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Publication number
PT109359A
PT109359A PT109359A PT10935916A PT109359A PT 109359 A PT109359 A PT 109359A PT 109359 A PT109359 A PT 109359A PT 10935916 A PT10935916 A PT 10935916A PT 109359 A PT109359 A PT 109359A
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PT
Portugal
Prior art keywords
sample
portable device
detection
deoxyribonucleic acid
present disclosure
Prior art date
Application number
PT109359A
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Portuguese (pt)
Inventor
Carlos Marcial Gomes Newton
Rogério Lopes Dos Santos António
Jesus Oliveira Vanessa
José Riso Das Costa Coelho Francisco
Original Assignee
Univ Aveiro
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Publication date
Application filed by Univ Aveiro filed Critical Univ Aveiro
Priority to PT109359A priority Critical patent/PT109359A/en
Priority to PCT/IB2017/052570 priority patent/WO2017191575A1/en
Publication of PT109359A publication Critical patent/PT109359A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A PRESENTE DIVULGAÇÃO REFERE-SE A UM DISPOSITIVO PORTÁTIL (1) PARA DETEÇÃO DE UM ÁCIDO DESOXIRRIBONUCLEICO DE UMA AMOSTRA. O PRESENTE DISPOSITIVO COMPREENDE OS SEGUINTES ELEMENTOS: UMA HASTE E UMA PEÇA DESCARTÁVEL (2) LOCALIZADA NUMA DAS EXTREMIDADES DO REFERIDO DISPOSITIVO. A REFERIDA PEÇA DESTACÁVEL (2) É DENOMINADA TAMPA-MEMBRANA E É POR SUA VEZ ACOPLADA, POR EXEMPLO, A UM MICROTUBO (2A). A TAMPAMEMBRANA DO DISPOSITIVO PORTÁTIL PARA DETEÇÃO DE UM ADN/PINCEL INDICADOR COMPREENDE, POR SUA VEZ, TRÊS ELEMENTOS DISTINTOS, UMA ZONA DE CLIPAGEM DA TAMPA AO MICROTUBO (2B), UMA ZONA DE ABSORÇÃO DA SOLUÇÃO DE REAÇÃO E AMOSTRA DE ADN (2C) E UMA ZONA DE CLIPAGEM AO CORPO DO PINCEL (2D). O DISPOSITIVO COMPREENDE AINDA UM INDICADOR DE REAÇÃO (3). A PRESENTE DIVULGAÇÃO TAMBÉM DIZ RESPEITO A UM MÉTODO PARA DETEÇÃO ESPECÍFICA DE ETIQUETAS MOLECULARES CONTENDO MOLÉCULAS DE ÁCIDO DESOXIRRIBONUCLEICO UTILIZADAS NA MARCAÇÃO, RASTREABILIDADE E AUTENTICIDADE DE DIVERSOS MATERIAIS, EM PARTICULAR MATERIAIS BIOLÓGICOS, NÃO BIOLÓGICOS E MEIOS LÍQUIDOS.The present disclosure relates to a portable device (1) for the detection of a deoxyribonucleic acid of a sample. THIS DEVICE COMPRISES THE FOLLOWING ELEMENTS: A SHOULDER AND A DISPOSABLE PART (2) LOCATED IN ONE OF THE EXTREMITIES OF THE REFERENCE DEVICE. The above-mentioned insert (2) is called a membrane sleeve and is in turn coupled, for example, to a micro-hub (2A). The capac- ity of the DNA / pincer detection device comprises, in turn, three different elements, a zone of clipping from the jacket to the micro-tube (2B), an area of reaction solution sol- ution and DNA sample (2C) AND A CLIPPING AREA TO THE BRUSH BODY (2D). THE DEVICE INCLUDES A REACTION INDICATOR (3). The present disclosure also relates to a method for the specific detection of molecule labels containing deoxyribonucleic acid molecules used in the marking, traceability and authenticity of various materials, in particular biological, non-biological and liquid media.

Description

DESCRIÇÃODESCRIPTION

DISPOSITIVO PORTÁTIL, MÉTODO PARA LEITURA DE ETIQUETAS DEPORTABLE DEVICE, METHOD FOR READING LABELS OF

AUTENTICAÇÃO E SEUS USOSAUTHENTICATION AND ITS USES

Dominio técnico [0001] A presente divulgação refere-se a um dispositivo portátil e a um método para deteção especifica de etiquetas moleculares contendo moléculas de ácido desoxirribonucleico (ADN) utilizadas na marcação, rastreabilidade e autenticidade de diversos materiais, em particular materiais biológicos, não biológicos e meios liquidos. A presente divulgação insere-se assim, no dominio das tecnologias para rastreabilidade e autenticação, com aplicação em qualquer tipo de atividade em que seja necessário a identificação de materiais marcados com ADN.Technical Field The present disclosure relates to a portable device and to a method for the specific detection of molecular tags containing deoxyribonucleic acid (DNA) molecules used in the marking, traceability and authenticity of various materials, in particular biological materials, not biological and liquid media. The present disclosure thus applies, in the domain of technologies for traceability and authentication, with application in any type of activity where it is necessary the identification of materials marked with DNA.

Antecedentes [0002] A entrada de produtos falsificados nas cadeias de abastecimento está a ganhar proporções nunca antes registadas, com consequências alarmantes nas receitas de empresas e na saúde e segurança de consumidores em todo o mundo. Existe, assim, uma necessidade crescente de tecnologias inovadoras que permitam combater a contrafação e proteger a população contra produtos contrafeitos e adulterados. Atualmente estão amplamente implementados vários sistemas de autenticação de produtos e certificação de sua origem. Os mais comuns recorrem à utilização de etiquetas adesivas com ou sem códigos de barras e etiquetas eletrónicas com identificação por radio-frequência (RFID). No entanto as várias técnicas de contrafação têm experimentado uma rápida evolução tornando desta forma vulneráveis os métodos tradicionais de autenticação. Em 15. Kit que compreende o dispositivo descrito em qualquer uma das reivindicações anteriores.Background [0002] Counterfeit products entering supply chains are gaining in unprecedented proportions, with alarming consequences on corporate revenues and consumer health and safety worldwide. There is thus a growing need for innovative technologies to combat counterfeiting and to protect the population against counterfeit and adulterated products. Several systems of product authentication and certification of its origin are currently widely implemented. The most common are the use of adhesive labels with or without bar codes and electronic tags with radio frequency identification (RFID). However, the various counterfeit techniques have been rapidly evolving, thus making traditional authentication methods vulnerable. A kit comprising the device described in any one of the preceding claims.

Data: 3 de maio de 2017 6. Dispositivo de acordo com qualquer uma das reivindicações anteriores em que o dispositivo compreende um segundo elemento destacável apto para ser acoplado à segunda extremidade do dispositivo e envolver o elemento de clipagem. 7. Dispositivo de acordo com qualquer uma das reivindicações anteriores em que o segundo elemento destacável é um microtubo. 8. Dispositivo de acordo com qualquer uma das reivindicações anteriores em que os elementos destacáveis são descartáveis. 9. Dispositivo de acordo com qualquer uma das reivindicações anteriores em que a membrana porosa de policarbonato é descartável. 10. Dispositivo de acordo com qualquer uma das reivindicações anteriores em que o dispositivo compreende ainda um indicador de reação, em particular um indicador colorimétrico de reação. 11. Dispositivo de acordo com a reivindicação anterior em que o indicador de reação está compreendido na solução de amplificação enzimática isotérmica. 12. Dispositivo de acordo com as reivindicações 10-11 em que o indicador de reação é um corante da família das cianinas. 13. Dispositivo de acordo com as reivindicações 10-12 em que o corante de reação é o azul de hidroxinaftol. 14. Dispositivo de acordo com qualquer uma das reivindicações anteriores em que a amostra é selecionada da seguinte lista: superfície de embalagens, produtos farmacêuticos e/ou produtos alimentares.A device according to any one of the preceding claims wherein the device comprises a second detachable member apt to be coupled to the second end of the device and engage the clip member. A device according to any one of the preceding claims wherein the second peelable member is a microtube. A device according to any one of the preceding claims wherein the detachable elements are disposable. A device according to any one of the preceding claims wherein the porous polycarbonate membrane is disposable. A device according to any one of the preceding claims wherein the device further comprises a reaction indicator, in particular a colorimetric indicator of reaction. The device of the preceding claim wherein the reaction indicator is comprised in the isothermal enzymatic amplification solution. A device according to claims 10-11 wherein the reaction indicator is a dye of the cyanine family. A device according to claims 10-12 wherein the reaction dye is hydroxynaphtol blue. A device according to any one of the preceding claims wherein the sample is selected from the following list: surface of packages, pharmaceuticals and / or food products.

Claims (5)

REIVINDICAÇÃOCLAIM 1. Dispositivo portátil para deteção de um ácido desoxirribonucleico de uma amostra, que compreende: uma haste com uma primeira extremidade e uma segunda extremidade; em que a primeira extremidade da haste é apta para ser manuseada por um utilizador e a segunda extremidade da haste compreende um elemento destacável; em que o referido elemento destacável compreende um elemento de clipagem que compreende duas posições, em que a primeira posição acopla o elemento de clipagem à segunda extremidade da referida haste e a segunda posição do elemento de clipagem acopla uma membrana porosa de policarbonato para suporte da amostra e de uma solução de amplificação enzimática isotérmica.A portable device for detecting deoxyribonucleic acid from a sample, comprising: a rod with a first end and a second end; wherein the first end of the rod is apt to be handled by a user and the second end of the rod comprises a detachable member; wherein said detachable member comprises a clip member comprising two positions, wherein the first position couples the clip member to the second end of said rod and the second position of the clip member engages a porous polycarbonate membrane to support the sample and an isothermal enzymatic amplification solution. 2. Dispositivo de acordo com a reivindicação anterior em que a membrana porosa de policarbonato compreende uma porosidade entre 0,05-8 ym, de preferência 0,2-8 ym, ainda de mais preferência 1-5 ym.A device according to the preceding claim wherein the porous polycarbonate membrane comprises a porosity between 0.05-8 and m, preferably 0.2-8 and m, even more preferably 1-5 and m. 3. Dispositivo de acordo com as reivindicações anteriores em que a membrana porosa de policarbonato compreende um revestimento.The device of the preceding claims wherein the porous polycarbonate membrane comprises a coating. 4. Dispositivo de acordo com a reivindicação anterior em que o referido revestimento é um revestimento de polivinilpirrolidona.The device of the preceding claim wherein said coating is a polyvinylpyrrolidone coating. 5. Dispositivo de acordo com as reivindicações 3-4 em que o revestimento de polivinilpirrolidona compreende uma espessura entre 0,5-1,5 ym, de preferência 1 ym.A device according to claims 3-4 wherein the polyvinylpyrrolidone coating comprises a thickness between 0.5-1.5 andm, preferably 1 andm.
PT109359A 2016-05-03 2016-05-03 PORTABLE DEVICE, METHOD FOR READING AUTHENTICATION LABELS AND ITS USES. PT109359A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PT109359A PT109359A (en) 2016-05-03 2016-05-03 PORTABLE DEVICE, METHOD FOR READING AUTHENTICATION LABELS AND ITS USES.
PCT/IB2017/052570 WO2017191575A1 (en) 2016-05-03 2017-05-03 Portable device, method for reading authentication labels and uses thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PT109359A PT109359A (en) 2016-05-03 2016-05-03 PORTABLE DEVICE, METHOD FOR READING AUTHENTICATION LABELS AND ITS USES.

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PT109359A true PT109359A (en) 2017-11-03

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PT109359A PT109359A (en) 2016-05-03 2016-05-03 PORTABLE DEVICE, METHOD FOR READING AUTHENTICATION LABELS AND ITS USES.

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Family Cites Families (14)

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US5645801A (en) 1993-10-21 1997-07-08 Abbott Laboratories Device and method for amplifying and detecting target nucleic acids
US6468751B1 (en) 1994-08-03 2002-10-22 Mosaic Technologies, Inc. Method and apparatus for performing amplification of nucleic acid on supports
US5837466A (en) 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
US6004057A (en) * 1997-11-24 1999-12-21 Fulop; Jacqueline I. Marking and eradicating instrument and method of use of same
AR031640A1 (en) 2000-12-08 2003-09-24 Applied Research Systems ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT
US7115301B2 (en) 2001-04-09 2006-10-03 Rixflex Holdings Limited Method of marking solid or liquid substances with nucleic acid for anti-counterfeiting and authentication
US8415164B2 (en) 2003-04-16 2013-04-09 Apdn (B.V.I.) Inc. System and method for secure document printing and detection
US7229487B2 (en) * 2004-01-14 2007-06-12 Sanford, L.P. Writing instruments with eradicable inks and eradicating fluids
ITMO20040126A1 (en) 2004-05-21 2004-08-21 Bioanalisi Ct Sud S N C Di Per SYSTEM FOR THE RESEARCH AND IDENTIFICATION OF PATHOGENIC AGENTS
WO2011150115A2 (en) * 2010-05-25 2011-12-01 Lawrence Livermore National Security, Llc Methods and apparatus for point-of-care detection of nucleic acid in a sample
US9469871B2 (en) * 2011-04-14 2016-10-18 Corporos Inc. Methods and apparatus for point-of-care nucleic acid amplification and detection
EP2773775A4 (en) 2011-11-04 2015-05-27 Diagnostics For All Inc Low cost, disposable molecular diagnostic devices
US9297032B2 (en) 2012-10-10 2016-03-29 Apdn (B.V.I.) Inc. Use of perturbants to facilitate incorporation and recovery of taggants from polymerized coatings
WO2015026254A1 (en) 2013-08-23 2015-02-26 Universidade De Aveiro Molecular tag containing dna molecules and process for marking and identifying the tag

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