PT102383A - Culturing of cells, particularly neuronal cells, useful for transplantation for treating neurological disorders such as Parkinson's disease or for gene therapy - Google Patents
Culturing of cells, particularly neuronal cells, useful for transplantation for treating neurological disorders such as Parkinson's disease or for gene therapyInfo
- Publication number
- PT102383A PT102383A PT10238399A PT10238399A PT102383A PT 102383 A PT102383 A PT 102383A PT 10238399 A PT10238399 A PT 10238399A PT 10238399 A PT10238399 A PT 10238399A PT 102383 A PT102383 A PT 102383A
- Authority
- PT
- Portugal
- Prior art keywords
- cells
- culturing
- differentiated
- neuronal cells
- incubating
- Prior art date
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A method of culturing cells comprises proliferating precursor cells, by incubating them in proliferating medium which includes basic fibroblast growth factor (bFGF), and differentiating the precursor cells, by incubating them in an incubation vessel which contains differentiation medium, to form a reaggregation of differentiated cells not adhered to any surface of the incubation vessel. Independent claims are also included for the following: (1) a method of culturing cells comprising incubating the cells in proliferating medium which includes bFGF; (2) a method of culturing differentiating cells, comprising incubating precursor cells in an incubation vessel which contains differentiation medium to form a reaggregation of differentiated cells that are not adhered to any surface of the incubation vessel; (3) a method of treating a patient with neurological disorder comprising: (a) culturing differentiated neuronal cells, the culturing comprising the novel method; and (b) administering the differentiated neuronal cells to a patient; (4) a method of introducing a gene product into a brain of a patient comprising: (a) transforming neuronal precursor cells; (b) culturing the transformed neuronal cells, the culturing comprising the novel method; and (c) administering the differentiated transformed neuronal cells into a patient; (5) an assay for a substance comprising: (a) culturing differentiated neuronal cells, the culturing comprising the novel method; (b) exposing the differentiated neuronal cells to the substance; and (c) observing the effect of the substance on the differentiated neuronal cells; and (6) a cell culture comprising 80-95% differentiated neuronal cells and less than 5% glial cells. ACTIVITY : Nootropic; Neuorprotective; Antiparkinsonian; Anticonvulsant; Neuroleptic. MECHANISM OF ACTION : None given.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PT10238399A PT102383B (en) | 1999-12-03 | 1999-12-03 | VARIABLE LENSES FOR HIDDEN |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PT10238399A PT102383B (en) | 1999-12-03 | 1999-12-03 | VARIABLE LENSES FOR HIDDEN |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PT102383A true PT102383A (en) | 2001-06-29 |
| PT102383B PT102383B (en) | 2003-07-31 |
Family
ID=20085906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT10238399A PT102383B (en) | 1999-12-03 | 1999-12-03 | VARIABLE LENSES FOR HIDDEN |
Country Status (1)
| Country | Link |
|---|---|
| PT (1) | PT102383B (en) |
-
1999
- 1999-12-03 PT PT10238399A patent/PT102383B/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| PT102383B (en) | 2003-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB1A | Laying open of patent application |
Effective date: 20000128 |
|
| MM3A | Annulment or lapse |
Free format text: LAPSE DUE TO NON-PAYMENT OF FEES Effective date: 20040630 |