PL59083B1 - - Google Patents

Description

In this trial, the calf was infected by intramuscularly injecting two 10 ml aliquots of the vaccine product with a 3-week interval between injections. Some calves have been contaminated with the unconjugated mixture, others - the concentrated mixture. The guinea pigs were administered equivalent doses of 1 ml, using the same interval between injections. Appropriate non-contaminated control calves and guinea pigs were also prepared. Haemagglutination inhibition titer results were collected from all calves and guinea pigs. The titer values for the four batches of the tested vaccine are summarized in the table below: Table Lot No. 1 2 3 4 Vaccine - (control) Non-seasonal - (control) Non-seasonal - ( control) Non-Seasoned - (control) Non-Seasoned Calf a 7 & 160 508 0 B07 508 25 807 320 160 807 255 guinea pigs b (Nebraska) and 22 49 ll3 9, 32 60 10 103 198 11 1297 260 guinea pigs b (Indiana) 5d 132 236 - 132 539 - 321 906, - 202 260 65 Meaning of letter symbols: a - average of three calves b - average of 8-10 guinea pigs c - each of the numbers means the second number in the ratio of HI 1: N, where N is a number in the table d - only one cage of Indiana control animals was used for the study, but all four runs were tested simultaneously. These and other data illustrate the potency of the vaccine mixture of the invention and that both guinea pigs and the calf can be used to measure the effectiveness of a vaccine. This discovery was also confirmed by means of statistical analysis. PL

Claims (6)

Claims 1. A method for the preparation of a bovine transport fever vaccine containing an inactivated mixture of Myxovirus parainfluenza-3 virus and Pasteurella microorganisms with an adjuvant, characterized in that it is an adjuvant to the culture mixture of Myxovirus parainfluenza-3, In the culture of Pasteurella multocida and in the culture of Pasteurella hemolytica, a gel of aluminum hydroxide, aluminum oxide in dispersed form or aluminum phosphate are added, the amount of adjuvant selected so as to obtain a preparation containing aluminum ions in an amount chemically equivalent to the concentration obtained by adding about 10-50 ml of an aqueous suspension containing 1.3% by weight of Al 2 O 3 per 100 ml of microbial culture and that the preparation has a density 5-10 times higher than the McFarland sample No. 1 according to the McFarland test scale, with whereby the microorganisms are subjected individually or in a mixture to inactivate before or after the addition of the adjuvant. After treatment with a known inactivating agent, the excess of the inactivating agent is then neutralized, and the preparation is supplemented with bacteriostatic and fungistatic amounts of antisease, acceptable for parenteral use. 2. The method according to claim A process as claimed in claim 1, characterized in that formaldehyde is used as the inactivating agent. 3. The method according to p. A method as claimed in any one of claims 1 and 2, characterized in that the excess of the inactivating agent is neutralized with an aqueous alkali metal bisulfite solution. 4. The method according to p. A process as claimed in any one of claims 1 to 3, characterized in that the pH of the combined viral-bacterial mixture is adjusted to 6.4-6.8 before mixing the mixture with the adjuvant. 5. The method according to p. The method of claim 4, wherein the liquid tissue culture of the Myxovirus parainfluenza-3 virus is frozen prior to mixing with bacterial cultures. 6. The method according to p. A method according to claim 1, characterized in that the known method of tissue culture from the kidney is multiplied by the known method of tissue culture from the kidney until 50-70% of the cells show a characteristic cytopathogenic effect, and the cultures are mixed with the bacterial cultures used in an amount not greater than 25% by volume of the obtained viral-bacterial mixture, the density of which is adjusted to equal about 7 to 8 times the density of a McFarland No. 1 tube, and water adjuvanted with a slurry of aluminum hydroxide gel, suspend 1 to 2% by weight of aluminum hydroxide and the resulting mixture is mixed with formaldehyde in such an amount to obtain a concentration of this compound of 0.075-0.125% by weight, and then to the virally inactivated mixture. In the presence of an adjuvanted bacterial agent, the sodium salt of salicylic acid ethyl mercury ester is added in an amount of about 0.4-0.6 ml of a 10% solution per 1 liter of product. ZG "Ruch" Warsaw, res. 1363-69, printed number 230 PL