PL445690A1 - System for detecting protein in a sample of biological material in liquid form and method for detecting protein carried out using this system - Google Patents

System for detecting protein in a sample of biological material in liquid form and method for detecting protein carried out using this system

Info

Publication number
PL445690A1
PL445690A1 PL445690A PL44569023A PL445690A1 PL 445690 A1 PL445690 A1 PL 445690A1 PL 445690 A PL445690 A PL 445690A PL 44569023 A PL44569023 A PL 44569023A PL 445690 A1 PL445690 A1 PL 445690A1
Authority
PL
Poland
Prior art keywords
detecting protein
carried out
sample
measuring cuvette
liquid form
Prior art date
Application number
PL445690A
Other languages
Polish (pl)
Inventor
Daria Majchrowicz
Bartłomiej Ferra
Jakub Gierowski
Patryk Sokołowski
Adam Mazikowski
Łukasz Meironke
Sandra Pawłowska
Original Assignee
Politechnika Gdańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Gdańska filed Critical Politechnika Gdańska
Priority to PL445690A priority Critical patent/PL445690A1/en
Publication of PL445690A1 publication Critical patent/PL445690A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/34—Genitourinary disorders
    • G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Przedmiotem wynalazku jest układ do wykrywania białka, w szczególności albuminy w próbce materiału biologicznego w postaci płynnej, w szczególności moczu, składający się ze źródła światła optycznego (1), którym jest laser, światłowód (2) doprowadzający wiązkę wytworzoną za pomocą źródła światła optycznego (1) do kuwety pomiarowej (4) ze sfunkcjonalizowaną warstwą (3) zawierającą przeciwciała oraz detektor (5) Przedmiotem wynalazku jest również sposób wykrywania białka, za pomocą układu. Pierwszy pomiar referencyjny prowadzony jest w kuwecie pomiarowej (4) ze sfunkcjonalizowaną przeciwciałami warstwą bez badanej substancji, natomiast drugi pomiar przeprowadza się w kuwecie pomiarowej (4) z roztworem z badaną substancją Na podstawie analizy poziomów natężeń widm optycznych określa się czy do powierzchni kuwety pomiarowej (4) przyczepiło się białko a wykrycie białka powoduje spadek natężenia sygnału.The subject of the invention is a system for detecting protein, in particular albumin in a sample of biological material in liquid form, in particular urine, consisting of an optical light source (1), which is a laser, an optical fiber (2) delivering a beam generated by the optical light source (1) to a measuring cuvette (4) with a functionalized layer (3) containing antibodies, and a detector (5). The subject of the invention is also a method for detecting protein, using the system. The first reference measurement is carried out in a measuring cuvette (4) with a functionalized layer of antibodies without a tested substance, while the second measurement is carried out in a measuring cuvette (4) with a solution with a tested substance. Based on the analysis of the intensity levels of optical spectra, it is determined whether a protein has attached to the surface of the measuring cuvette (4), and the detection of protein causes a decrease in the signal intensity.

PL445690A 2023-07-27 2023-07-27 System for detecting protein in a sample of biological material in liquid form and method for detecting protein carried out using this system PL445690A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL445690A PL445690A1 (en) 2023-07-27 2023-07-27 System for detecting protein in a sample of biological material in liquid form and method for detecting protein carried out using this system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL445690A PL445690A1 (en) 2023-07-27 2023-07-27 System for detecting protein in a sample of biological material in liquid form and method for detecting protein carried out using this system

Publications (1)

Publication Number Publication Date
PL445690A1 true PL445690A1 (en) 2025-02-03

Family

ID=94381255

Family Applications (1)

Application Number Title Priority Date Filing Date
PL445690A PL445690A1 (en) 2023-07-27 2023-07-27 System for detecting protein in a sample of biological material in liquid form and method for detecting protein carried out using this system

Country Status (1)

Country Link
PL (1) PL445690A1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001071322A2 (en) * 2000-03-22 2001-09-27 Goh M Cynthia Method and apparatus for multiple-analyte assay
WO2017024163A1 (en) * 2015-08-04 2017-02-09 Abaxis, Inc. Signal amplification in solution-based plasmonic specific-binding partner assays

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001071322A2 (en) * 2000-03-22 2001-09-27 Goh M Cynthia Method and apparatus for multiple-analyte assay
WO2017024163A1 (en) * 2015-08-04 2017-02-09 Abaxis, Inc. Signal amplification in solution-based plasmonic specific-binding partner assays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VIGO, F. ET AL.: "Diagnostics 2023, 13, 27, opublikowany 22 December 2022", "VIBRATIONAL SPECTROSCOPY IN URINE SAMPLES AS A MEDICAL TOOL: REVIEW AND OVERVIEW ON THE CURRENT STATE-OF-THE-ART" *

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