PL438908A1 - Sposób hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), kompozycja czynników aktywnych zawartych w nadsączu otrzymanych tym sposobem i zastosowanie - Google Patents

Sposób hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), kompozycja czynników aktywnych zawartych w nadsączu otrzymanych tym sposobem i zastosowanie

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Publication number
PL438908A1
PL438908A1 PL438908A PL43890821A PL438908A1 PL 438908 A1 PL438908 A1 PL 438908A1 PL 438908 A PL438908 A PL 438908A PL 43890821 A PL43890821 A PL 43890821A PL 438908 A1 PL438908 A1 PL 438908A1
Authority
PL
Poland
Prior art keywords
composition
stem cells
mesenchymal stem
msc
human mesenchymal
Prior art date
Application number
PL438908A
Other languages
English (en)
Inventor
Maria Paprocka
Honorata Kraśkiewicz
Agnieszka Krawczenko
Aleksandra Bielawska-Pohl
Leszek Masłowski
Wojciech Witkiewicz
Anna Czarnecka
Agnieszka Czyżewska-Buczyńska
Natalia Jędruchniewicz
Aleksandra Klimczak
Original Assignee
Instytut Immunologii I Terapii Doświadczalnej Im.Ludwika Hirszfelda Pan We Wrocławiu
Wojewódzki Szpital Specjalistyczny We Wrocławiu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Instytut Immunologii I Terapii Doświadczalnej Im.Ludwika Hirszfelda Pan We Wrocławiu, Wojewódzki Szpital Specjalistyczny We Wrocławiu filed Critical Instytut Immunologii I Terapii Doświadczalnej Im.Ludwika Hirszfelda Pan We Wrocławiu
Priority to PL438908A priority Critical patent/PL438908A1/pl
Publication of PL438908A1 publication Critical patent/PL438908A1/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/05—Adjuvants
    • C12N2501/052—Lipopolysaccharides [LPS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/24—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/25—Tumour necrosing factors [TNF]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Pain & Pain Management (AREA)
  • Dermatology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Wynalazek dotyczy sposobu hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), otrzymanych metodą podwójnej transfekcji plazmidami prowadzony w standardowych i modyfikowanych warunkach oraz kompozycji czynników otrzymanych sposobem według wynalazku do zastosowania w leczeniu chorób skóry, w medycynie regeneracyjnej oraz w immunoterapii do leczenia chorób przewlekłych skóry.
PL438908A 2021-09-09 2021-09-09 Sposób hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), kompozycja czynników aktywnych zawartych w nadsączu otrzymanych tym sposobem i zastosowanie PL438908A1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL438908A PL438908A1 (pl) 2021-09-09 2021-09-09 Sposób hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), kompozycja czynników aktywnych zawartych w nadsączu otrzymanych tym sposobem i zastosowanie

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL438908A PL438908A1 (pl) 2021-09-09 2021-09-09 Sposób hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), kompozycja czynników aktywnych zawartych w nadsączu otrzymanych tym sposobem i zastosowanie

Publications (1)

Publication Number Publication Date
PL438908A1 true PL438908A1 (pl) 2023-03-13

Family

ID=85480544

Family Applications (1)

Application Number Title Priority Date Filing Date
PL438908A PL438908A1 (pl) 2021-09-09 2021-09-09 Sposób hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), kompozycja czynników aktywnych zawartych w nadsączu otrzymanych tym sposobem i zastosowanie

Country Status (1)

Country Link
PL (1) PL438908A1 (pl)

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PL438908A1 (pl) Sposób hodowli immortalizowanych linii ludzkich mezenchymalnych komórek macierzystych (MSC), kompozycja czynników aktywnych zawartych w nadsączu otrzymanych tym sposobem i zastosowanie