PL437753A1 - Flow deproteinizer and method of deproteinization of liquid biological samples - Google Patents

Flow deproteinizer and method of deproteinization of liquid biological samples

Info

Publication number
PL437753A1
PL437753A1 PL437753A PL43775321A PL437753A1 PL 437753 A1 PL437753 A1 PL 437753A1 PL 437753 A PL437753 A PL 437753A PL 43775321 A PL43775321 A PL 43775321A PL 437753 A1 PL437753 A1 PL 437753A1
Authority
PL
Poland
Prior art keywords
flow
deproteinizer
protein
deproteinization
sup
Prior art date
Application number
PL437753A
Other languages
Polish (pl)
Inventor
Iga MALICKA
Izabela LEWIŃSKA
Michał Michalec
Łukasz Tymecki
Original Assignee
Uniwersytet Warszawski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Warszawski filed Critical Uniwersytet Warszawski
Priority to PL437753A priority Critical patent/PL437753A1/en
Priority to PCT/PL2022/000028 priority patent/WO2022231447A2/en
Publication of PL437753A1 publication Critical patent/PL437753A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/14—Preparation by elimination of some components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/28—Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/30—Control equipment
    • B01D21/32—Density control of clear liquid or sediment, e.g. optical control ; Control of physical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Deproteinizator przepływowy, zawierający przewód doprowadzający próbkę ciekłą zawierającą białko, komorę deproteinizacji oraz przewód wyprowadzający ciekłą próbkę zdeproteinizowaną, charakteryzuje się tym, że działa w trybie strąceniowym i jest wyposażony w układ wibracyjny zapewniający możliwość wytrząsania zawartości komory deproteinizacji (2), która ma objętość 1,2 - 1,8 cm<sup>3</sup>, korzystnie 1,5 cm<sup>3</sup>, i kształt graniastosłupa lub odwróconego naboju zorientowanego w osi pionowej, którego wysokość jest większa niż przekątna podstawy, dzięki czemu możliwe jest łatwe samorzutne rozdzielenie frakcji odbiałczonej od frakcji zawierającej osad białkowy, przy czym deproteinizator jest połączony z układem analitycznym za pomocą elastycznych przewodów umożliwiających wprowadzanie go w wibracje, a także zawiera układ odprowadzający próbkę zdeproteinizowaną, układ odprowadzający strącony osad zawierający białko, układ czyszczenia komory deproteinizacji oraz układ odpowietrzania, przy czym wszystkie kanały doprowadzające i odprowadzające wyposażone są w sterowane elektronicznie zawory, a także, korzystnie, układ optyczny pozwalający na pomiar mętności deproteinizowanej próbki. Sposób deproteinizacji ciekłych próbek biologicznych wykorzystujący reakcję denaturacji białek za pomocą czynnika wytrącającego, charakteryzuje się tym, że reakcję denaturacji białka zawartego w próbce biologicznej inicjuje się wprowadzeniem czynnika strąceniowego i prowadzi się w deproteinizatorze przepływowym opisanym powyżej. Deproteinizator przepływowy umożliwiający odbiałczenie próbek biologicznych w trybie przepływowym, pozwala na skrócenie czasu analizy i uproszczenie procedury przygotowania próbki w stosunku do odbiałczania przeprowadzanego w trybie manualnym, a także ograniczenie kosztów analizy i eksploatacji układu w stosunku do odbiałczania w trybie przepływowym za pomocą membran.The flow-through deproteinizer, comprising a protein-containing liquid sample supply line, a deproteinisation chamber and a deproteinisation liquid sample discharge line, is characterized in that it operates in a precipitation mode and is equipped with a vibrating system for shaking the content of the deproteinisation chamber (2), which has a volume of 1, 2 - 1.8 cm<sup>3</sup>, preferably 1.5 cm<sup>3</sup>, and the shape of a prism or inverted cartridge oriented in the vertical axis, the height of which is greater than the diagonal of the base, so that it is possible to easily spontaneously separate the deproteinized fraction from the fraction containing the protein sludge, the deproteinizer is connected to the analytical system by means of flexible hoses enabling it to vibrate, and also includes a system for removing the deproteinized sample, a system for removing the precipitated sludge containing protein, a system for cleaning the deproteinization chamber and the venting system, wherein all the inlet and outlet channels are equipped with electronically controlled valves and, preferably, an optical system for measuring the turbidity of the deproteinized sample. A method for deproteinizing liquid biological samples using a protein denaturation reaction with a precipitating agent is characterized in that the denaturation reaction of a protein contained in a biological sample is initiated by the introduction of a precipitating agent and carried out in the flow deproteinizer described above. The flow deproteinizer, which enables the deproteination of biological samples in the flow mode, shortens the analysis time and simplifies the sample preparation procedure in relation to manual deproteinization, as well as reduces the costs of analysis and operation of the system in relation to the flow deproteinization using membranes.

PL437753A 2021-04-29 2021-04-29 Flow deproteinizer and method of deproteinization of liquid biological samples PL437753A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL437753A PL437753A1 (en) 2021-04-29 2021-04-29 Flow deproteinizer and method of deproteinization of liquid biological samples
PCT/PL2022/000028 WO2022231447A2 (en) 2021-04-29 2022-04-29 Flow deproteinizer and a method of deproteinization of liquid biological samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL437753A PL437753A1 (en) 2021-04-29 2021-04-29 Flow deproteinizer and method of deproteinization of liquid biological samples

Publications (1)

Publication Number Publication Date
PL437753A1 true PL437753A1 (en) 2022-10-31

Family

ID=82839195

Family Applications (1)

Application Number Title Priority Date Filing Date
PL437753A PL437753A1 (en) 2021-04-29 2021-04-29 Flow deproteinizer and method of deproteinization of liquid biological samples

Country Status (2)

Country Link
PL (1) PL437753A1 (en)
WO (1) WO2022231447A2 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7166443B2 (en) * 2001-10-11 2007-01-23 Aviva Biosciences Corporation Methods, compositions, and automated systems for separating rare cells from fluid samples
WO2016118915A1 (en) * 2015-01-22 2016-07-28 Becton, Dickinson And Company Devices and systems for molecular barcoding of nucleic acid targets in single cells
DE102018211281A1 (en) * 2018-07-09 2020-01-09 Robert Bosch Gmbh Microfluidic device and method for diluting and separating particles of a sample
US10940477B2 (en) * 2018-07-19 2021-03-09 Micareo Taiwan Co., Ltd. Fluid sample collection and retrieval apparatus and microfluidic chip

Also Published As

Publication number Publication date
WO2022231447A3 (en) 2022-12-08
WO2022231447A2 (en) 2022-11-03

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