PL433032A1 - Method of preparing a carrier for immobilization of living microorganisms - Google Patents

Method of preparing a carrier for immobilization of living microorganisms

Info

Publication number
PL433032A1
PL433032A1 PL433032A PL43303220A PL433032A1 PL 433032 A1 PL433032 A1 PL 433032A1 PL 433032 A PL433032 A PL 433032A PL 43303220 A PL43303220 A PL 43303220A PL 433032 A1 PL433032 A1 PL 433032A1
Authority
PL
Poland
Prior art keywords
mixture
xanthan gum
immobilization
carrier
living microorganisms
Prior art date
Application number
PL433032A
Other languages
Polish (pl)
Other versions
PL240860B1 (en
Inventor
Anna Dzionek
Danuta Wojcieszyńska
Urszula Guzik
Original Assignee
Uniwersytet Śląski W Katowicach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Śląski W Katowicach filed Critical Uniwersytet Śląski W Katowicach
Priority to PL433032A priority Critical patent/PL240860B1/en
Publication of PL433032A1 publication Critical patent/PL433032A1/en
Publication of PL240860B1 publication Critical patent/PL240860B1/en

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem wynalazku jest sposób otrzymywania nośnika do immobilizacji żywych mikroorganizmów polegający na tym, że gumę ksantanową rozpuszcza się w roztworze chlorku litu (LiCl) o stężeniu 10-3 M, przy proporcji gumy do roztworu mieszczącej się w zakresie 20-33 g/L, po czym do mieszaniny dodaje się czynnik sieciujący w postaci dihydrazydu kwasu adypinowego (ADH) w ilości 10 g/L (gram/litr mieszaniny) oraz aktywator gumy ksantanowej w postaci chlorowodorku 1-etylo-3[3-dimetyloamino]propylokarbodiimidu (EDCl) w ilości od 20 do 30 g/L, korzystnie 25 g/L (gram/litr mieszaniny) i sieciuje się ją do momentu uzyskania usieciowanej gumy ksantanowej, po czym dializuje się ją usuwając niezwiązane cząsteczki dihydrazydu kwasu adypinowego (ADH) oraz chlorowodorku 1-etylo-3[3-dimetyloamino]propylokarbodiimidu(EDCl) i suszy w temperaturze do 70°C usuwając nadmiar wody.The subject of the invention is a method of obtaining a carrier for the immobilization of living microorganisms, consisting in dissolving xanthan gum in a lithium chloride (LiCl) solution with a concentration of 10-3 M, with a gum to solution ratio in the range of 20-33 g / L, after the mixture is followed by the adipic acid dihydrazide (ADH) crosslinking agent in the amount of 10 g / L (gram / liter of the mixture) and the xanthan gum activator in the form of 1-ethyl-3 [3-dimethylamino] propylcarbodiimide hydrochloride (EDCl) in the amount of from 20 to 30 g / L, preferably 25 g / L (gram / liter of the mixture) and crosslinked until a cross-linked xanthan gum is obtained, after which it is dialyzed to remove unbound adipic acid dihydrazide (ADH) and 1-ethyl hydrochloride. 3 [3-dimethylamino] propylcarbodiimide (EDCl) and dried up to 70 ° C to remove excess water.

PL433032A 2020-02-26 2020-02-26 Method of preparing a carrier for immobilization of living microorganisms PL240860B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL433032A PL240860B1 (en) 2020-02-26 2020-02-26 Method of preparing a carrier for immobilization of living microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL433032A PL240860B1 (en) 2020-02-26 2020-02-26 Method of preparing a carrier for immobilization of living microorganisms

Publications (2)

Publication Number Publication Date
PL433032A1 true PL433032A1 (en) 2021-08-30
PL240860B1 PL240860B1 (en) 2022-06-20

Family

ID=77561383

Family Applications (1)

Application Number Title Priority Date Filing Date
PL433032A PL240860B1 (en) 2020-02-26 2020-02-26 Method of preparing a carrier for immobilization of living microorganisms

Country Status (1)

Country Link
PL (1) PL240860B1 (en)

Also Published As

Publication number Publication date
PL240860B1 (en) 2022-06-20

Similar Documents

Publication Publication Date Title
DE602005024919D1 (en) BIOKOMPATIBLE NETWORKED GEL
KR101973715B1 (en) Autogenous Crack Healing Concrete Composition Using Microorganism, And Method for Manufacturing the Same
CN101921481B (en) Gel prepared by in situ crosslinking hyaluronic acid and polyaspartic acid and preparation method thereof
DE602005021400D1 (en) BIODEGRADABLE, SUPER ABSORBENT POLYMER HYDROGELES AND METHOD FOR THE PRODUCTION THEREOF
DE602004006892D1 (en) ANION-BONDING POLYMERS AND ITS USES
CN108047377B (en) Temperature-sensitive hydrogel and preparation method thereof
CN103881014B (en) The quick double-bang firecracker of a kind of high resilience answers the preparation method of POSS hybridized hydrogel
CN111253629B (en) Gel, complete set of raw materials and application thereof
JP2008536958A5 (en)
IT8320693A1 (en) Process for the immobilization of biological materials in condensed polymers of polyalkyleneimines, compounds containing an active and immobilized biological material and processes for the production of aspartic acid, phenylaniline and tryptophan
CN104356319A (en) Porous biological material using modified gelatin as crosslinking agent and preparation method of porous biological material
CN104829780A (en) Preparation method for high-strength hydrogel with rapid response to both pH value and temperature
CN104177636A (en) In-situ mineralized reinforced composite hydrogel and preparation method thereof
JP2018172234A (en) Microbe-encapsulated microcapsule, cement admixture including microbe-encapsulated microcapsule, and repair material of cement-based structure
CN111320769A (en) PH and redox dual-response drug-loaded hydrogel and preparation method and application thereof
CN100384923C (en) Process for preparing hyaluronic acid-chitosan crosslinked biocompatible materials
SE0201599D0 (en) microparticles
PL433032A1 (en) Method of preparing a carrier for immobilization of living microorganisms
JP2019210404A (en) Self-healing gel
KR101854438B1 (en) Self-healing concrete admixture and self-healing concrete using amine functionalized hydrogel scaffolds
CN107163263A (en) A kind of preparation method and application of even porous hydrogel
CN113861447A (en) Modified hydroxypropyl chitosan adhesive self-healing hydrogel and preparation method and application thereof
CN106620845A (en) Injectable bone material and preparation method thereof
DE60222174D1 (en) METHOD FOR PRODUCING A SENSITIZED EMULSION SPREADING MATERIAL
EA200971004A1 (en) METHOD OF MANUFACTURE OF MODIFIED SEMICTITIS OR CONTAINING SMEKTIT MATERIAL, THE ADAPTABLE WATER AND SURVEABLE WATER IN REVERSIBLE