PL432889A1 - Sposób wykrywania monomerów i agregatów białka, zastosowanie tioflawiny T do wykrywania monomerów i agregatów białka, tioflawina T do zastosowania w diagnozowaniu choroby związanej z agregacją białek, układ optyczny do wykrywania monomerów i agregatów białka oraz sposób generacji wzmocnionej emisji spontanicznej tioflawiny T - Google Patents
Sposób wykrywania monomerów i agregatów białka, zastosowanie tioflawiny T do wykrywania monomerów i agregatów białka, tioflawina T do zastosowania w diagnozowaniu choroby związanej z agregacją białek, układ optyczny do wykrywania monomerów i agregatów białka oraz sposób generacji wzmocnionej emisji spontanicznej tioflawiny TInfo
- Publication number
- PL432889A1 PL432889A1 PL432889A PL43288920A PL432889A1 PL 432889 A1 PL432889 A1 PL 432889A1 PL 432889 A PL432889 A PL 432889A PL 43288920 A PL43288920 A PL 43288920A PL 432889 A1 PL432889 A1 PL 432889A1
- Authority
- PL
- Poland
- Prior art keywords
- thioflavin
- aggregates
- protein
- detection
- monomers
- Prior art date
Links
- JADVWWSKYZXRGX-UHFFFAOYSA-M thioflavine T Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C1=[N+](C)C2=CC=C(C)C=C2S1 JADVWWSKYZXRGX-UHFFFAOYSA-M 0.000 title abstract 11
- 102000004169 proteins and genes Human genes 0.000 title abstract 9
- 108090000623 proteins and genes Proteins 0.000 title abstract 9
- 239000000178 monomer Substances 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 4
- 230000002269 spontaneous effect Effects 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000003745 diagnosis Methods 0.000 title abstract 2
- 201000010099 disease Diseases 0.000 title abstract 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract 2
- 230000003287 optical effect Effects 0.000 title abstract 2
- 230000004845 protein aggregation Effects 0.000 title abstract 2
- 239000007787 solid Substances 0.000 abstract 8
- 239000000203 mixture Substances 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 1
- 230000001678 irradiating effect Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
- G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4709—Amyloid plaque core protein
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
- G01N2800/2814—Dementia; Cognitive disorders
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Neurology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Neurosurgery (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Przedmiotem wynalazku jest sposób wykrywania monomerów i agregatów białka, obejmujący następujące etapy: a) doprowadzenie do kontaktu tioflawiny T i białka, którego monomer lub agregat ma być wykrywany, z wytworzeniem mieszaniny tioflawina T - białko; b) wylanie i równomierne rozprowadzenie mieszaniny wytworzonej w etapie a) na stałym podłożu oraz wysuszenie z uzyskaniem stałej warstwy; c) naświetlanie stałej warstwy uzyskanej w etapie b) wiązką impulsów laserowych o gęstości energii w zakresie od 1 mJ/cm2 do 10 mJ/cm2 z rejestrowaniem wzmocnionej emisji spontanicznej. Przedmiotem wynalazku jest również zastosowanie tioflawiny T w postaci unieruchomionej na stałym podłożu do wykrywania monomerów i agregatów białka oraz tioflawina T unieruchomiona na stałym podłożu do zastosowania w diagnozowaniu choroby związanej z agregacją białek. Kolejnym przedmiotem wynalazku jest układ optyczny do wykrywania monomerów i agregatów białka, zawierający tioflawinę T oraz białko, którego monomer lub agregat ma być wykrywany, charakteryzujący się tym, że jest w postaci unieruchomionej na stałym podłożu. Przedmiotem wynalazku jest również sposób generacji wzmocnionej emisji spontanicznej tioflawiny T obejmujący następujące etapy: a) unieruchomienie tioflawiny T na stałym podłożu, oraz b) naświetlanie tioflawiny T unieruchomionej na stałym podłożu wiązką impulsów laserowych o gęstości energii w zakresie od 1 mJ/cm2 do 10 mJ/cm2 z rejestrowaniem wzmocnionej emisji spontanicznej.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL432889A PL241595B1 (pl) | 2020-02-12 | 2020-02-12 | Sposób, zestaw oraz zastosowanie tioflawiny T do wykrywania monomerów i agregatów białka, tioflawina T do zastosowania w diagnozowaniu choroby związanej z agregacją białek oraz sposób generacji wzmocnionej emisji spontanicznej tioflawiny T |
| PCT/PL2021/050005 WO2021162565A1 (en) | 2020-02-12 | 2021-02-12 | Method of detecting protein monomers and aggregates, use of thioflavin t for detecting protein monomers and aggregates, thioflavin t for use in diagnosis of a disease associated with protein aggregation, optical system for detecting protein monomers and aggregates and method of generating amplified spontaneous emission of thioflavin t |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL432889A PL241595B1 (pl) | 2020-02-12 | 2020-02-12 | Sposób, zestaw oraz zastosowanie tioflawiny T do wykrywania monomerów i agregatów białka, tioflawina T do zastosowania w diagnozowaniu choroby związanej z agregacją białek oraz sposób generacji wzmocnionej emisji spontanicznej tioflawiny T |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL432889A1 true PL432889A1 (pl) | 2021-08-16 |
| PL241595B1 PL241595B1 (pl) | 2022-10-31 |
Family
ID=75530132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL432889A PL241595B1 (pl) | 2020-02-12 | 2020-02-12 | Sposób, zestaw oraz zastosowanie tioflawiny T do wykrywania monomerów i agregatów białka, tioflawina T do zastosowania w diagnozowaniu choroby związanej z agregacją białek oraz sposób generacji wzmocnionej emisji spontanicznej tioflawiny T |
Country Status (2)
| Country | Link |
|---|---|
| PL (1) | PL241595B1 (pl) |
| WO (1) | WO2021162565A1 (pl) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201805466D0 (en) * | 2018-04-03 | 2018-05-16 | Vukojevic Vladana | Methods |
-
2020
- 2020-02-12 PL PL432889A patent/PL241595B1/pl unknown
-
2021
- 2021-02-12 WO PCT/PL2021/050005 patent/WO2021162565A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021162565A1 (en) | 2021-08-19 |
| PL241595B1 (pl) | 2022-10-31 |
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