PL425169A1 - Method for obtaining biological implant for regeneration of cartilaginous tissue - Google Patents
Method for obtaining biological implant for regeneration of cartilaginous tissueInfo
- Publication number
- PL425169A1 PL425169A1 PL425169A PL42516918A PL425169A1 PL 425169 A1 PL425169 A1 PL 425169A1 PL 425169 A PL425169 A PL 425169A PL 42516918 A PL42516918 A PL 42516918A PL 425169 A1 PL425169 A1 PL 425169A1
- Authority
- PL
- Poland
- Prior art keywords
- chondrocytes
- regeneration
- biological implant
- cartilaginous tissue
- obtaining biological
- Prior art date
Links
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- Materials For Medical Uses (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób otrzymywania biologicznego implantu do regeneracji tkanki chrzęstnej, obejmujący etapy: izolację chondrocytów z tkanki chrzęstnej, hodowlę wyizolowanych chondrocytów, umieszczania i prowadzenia hodowli chondrocytów na powierzchni membrany opartej na pochodnej kwasu hialuronowego. Sposób ten charakteryzuje się tym, że w kolejnym etapie prowadzi się wizualizację chondrocytów za pomocą roztworu trypsyny o stężeniu 0.25% i barwnika fluorescencyjnego silnie wiążącego się do DNA oraz przeprowadza się ocenę zagęszczenia chondrocytów na powierzchni membrany opartej na pochodnej kwasu hialuronowego poprzez zastosowanie trawienia enzymatycznego.The subject of the application is a method of obtaining a biological implant for the regeneration of cartilage tissue, comprising the steps of: isolating chondrocytes from cartilage tissue, culturing isolated chondrocytes, placing and culturing chondrocytes on the surface of a membrane based on a hyaluronic acid derivative. This method is characterized in that in the next step chondrocytes are visualized with a trypsin solution with a concentration of 0.25% and a fluorescent dye strongly binding to DNA, and the chondrocyte density on the surface of the membrane based on hyaluronic acid derivative is assessed by enzymatic digestion.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL425169A PL242834B1 (en) | 2018-04-10 | 2018-04-10 | Method for obtaining biological implant for regeneration of cartilaginous tissue |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL425169A PL242834B1 (en) | 2018-04-10 | 2018-04-10 | Method for obtaining biological implant for regeneration of cartilaginous tissue |
Publications (2)
Publication Number | Publication Date |
---|---|
PL425169A1 true PL425169A1 (en) | 2019-10-21 |
PL242834B1 PL242834B1 (en) | 2023-05-02 |
Family
ID=68238637
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL425169A PL242834B1 (en) | 2018-04-10 | 2018-04-10 | Method for obtaining biological implant for regeneration of cartilaginous tissue |
Country Status (1)
Country | Link |
---|---|
PL (1) | PL242834B1 (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1201749A1 (en) * | 2000-08-29 | 2002-05-02 | MERCK PATENT GmbH | Method for producing cartilage implants using chondrocytes grown in-vitro |
WO2003092542A2 (en) * | 2002-05-01 | 2003-11-13 | Verigen Ag | Injectable chondrocyte implant |
WO2006045330A1 (en) * | 2004-10-27 | 2006-05-04 | Tetec-Tissue Engineering Technologies Aktiengesellschaft | Implant for repairing a cartilage defect |
PL213865B1 (en) * | 2007-09-03 | 2013-05-31 | Regionalne Ct Krwiodawstwa I Krwiolecznictwa | The manner of obtaining of autogeneous explant of chondrocites in tissue glue based on fibrinogen |
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2018
- 2018-04-10 PL PL425169A patent/PL242834B1/en unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1201749A1 (en) * | 2000-08-29 | 2002-05-02 | MERCK PATENT GmbH | Method for producing cartilage implants using chondrocytes grown in-vitro |
WO2003092542A2 (en) * | 2002-05-01 | 2003-11-13 | Verigen Ag | Injectable chondrocyte implant |
WO2006045330A1 (en) * | 2004-10-27 | 2006-05-04 | Tetec-Tissue Engineering Technologies Aktiengesellschaft | Implant for repairing a cartilage defect |
PL213865B1 (en) * | 2007-09-03 | 2013-05-31 | Regionalne Ct Krwiodawstwa I Krwiolecznictwa | The manner of obtaining of autogeneous explant of chondrocites in tissue glue based on fibrinogen |
Non-Patent Citations (1)
Title |
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CHEN TIE, CHONDROGENESIS OF BONE MARROW MESENCHYMAL STEM CELLS CHONDROCYTES AND ADIPOSE-DERIVED ADULT STROMAL CELLS IN THREE-DIMENSIONAL SCAFFOLDS, 2010 * |
Also Published As
Publication number | Publication date |
---|---|
PL242834B1 (en) | 2023-05-02 |
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PL425169A1 (en) | Method for obtaining biological implant for regeneration of cartilaginous tissue |