PL425134A1 - Sekwencja nukleotydowa syntetycznego genu Cas9, kaseta kierująca sgRNA do edytowania genomu roślinnego i wydajny system do ukierunkowanej mutagenezy wybranego genomu roślinnego - Google Patents

Sekwencja nukleotydowa syntetycznego genu Cas9, kaseta kierująca sgRNA do edytowania genomu roślinnego i wydajny system do ukierunkowanej mutagenezy wybranego genomu roślinnego

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Publication number
PL425134A1
PL425134A1 PL425134A PL42513418A PL425134A1 PL 425134 A1 PL425134 A1 PL 425134A1 PL 425134 A PL425134 A PL 425134A PL 42513418 A PL42513418 A PL 42513418A PL 425134 A1 PL425134 A1 PL 425134A1
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Poland
Prior art keywords
plant genome
sgrna
editing
nucleotide sequence
directed mutagenesis
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PL425134A
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English (en)
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PL235163B1 (pl
Inventor
Sebastian Gasparis
Wacław ORCZYK
Maciej Jończyk
Paweł Sowiński
Anna Nadolska-Orczyk
Original Assignee
Instytut Hodowli I Aklimatyzacji Roślin-Państwowy Instytut Badawczy
Uniwersytet Warszawski
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Application filed by Instytut Hodowli I Aklimatyzacji Roślin-Państwowy Instytut Badawczy, Uniwersytet Warszawski filed Critical Instytut Hodowli I Aklimatyzacji Roślin-Państwowy Instytut Badawczy
Priority to PL425134A priority Critical patent/PL235163B1/pl
Publication of PL425134A1 publication Critical patent/PL425134A1/pl
Publication of PL235163B1 publication Critical patent/PL235163B1/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22062—Caspase-9 (3.4.22.62)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Przedmiotem zgłoszenia jest syntetyczny gen endonukleazy Cas9 posiadający sekwencję nukleotydową przedstawioną jako sekw.nr1. Zgłoszenie obejmuje także pustą kasetę kierująca sgRNA, oraz wypełnioną kasetę kierującą sgRNA. Ponadto przedmiotem zgłoszenia jest też zestaw do wytwarzania systemu Cas9/sgRNA oraz sposób edytowania genomu rośliny jednoliściennej, zwłaszcza jęczmienia.
PL425134A 2018-04-05 2018-04-05 Sekwencja nukleotydowa syntetycznego genu Cas9, kaseta kierująca sgRNA do edytowania genomu roślinnego i wydajny system do ukierunkowanej mutagenezy wybranego regionu genomu roślinnego PL235163B1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL425134A PL235163B1 (pl) 2018-04-05 2018-04-05 Sekwencja nukleotydowa syntetycznego genu Cas9, kaseta kierująca sgRNA do edytowania genomu roślinnego i wydajny system do ukierunkowanej mutagenezy wybranego regionu genomu roślinnego

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL425134A PL235163B1 (pl) 2018-04-05 2018-04-05 Sekwencja nukleotydowa syntetycznego genu Cas9, kaseta kierująca sgRNA do edytowania genomu roślinnego i wydajny system do ukierunkowanej mutagenezy wybranego regionu genomu roślinnego

Publications (2)

Publication Number Publication Date
PL425134A1 true PL425134A1 (pl) 2019-10-07
PL235163B1 PL235163B1 (pl) 2020-06-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
PL425134A PL235163B1 (pl) 2018-04-05 2018-04-05 Sekwencja nukleotydowa syntetycznego genu Cas9, kaseta kierująca sgRNA do edytowania genomu roślinnego i wydajny system do ukierunkowanej mutagenezy wybranego regionu genomu roślinnego

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PL (1) PL235163B1 (pl)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2771468A1 (en) * 2012-12-12 2014-09-03 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions for sequence manipulation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2771468A1 (en) * 2012-12-12 2014-09-03 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions for sequence manipulation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
E. KAPUSI, HERITABLE GENOMIC FRAGMENT DEELETIONS AND SMALL INDELS IN THE PUTATIVE ENGASE GENE INDUCED BY CRISPR/CAS9 IN BARLEY, 2017 *
LEI S. QI, REPURPOSING CRISPR AS AN RNA-GUIDED PLATFORM FOR SEQUENCE-SPECIFIC CONTROL OF GENE EXPRESSLON, 2013 *
SI NIAN CHAR, AN AGROBACTERIUM-DELIVERED CRISPR/CAS9 SYSTEM FOR HIGH-FREQUENCY TARGETED MUTAGENESIS IN MAIZE, 2017 *
XINGLIANG MA, CRISPR/CAS9 PLATFORMS FOR GENOME EDITING IN PLANTS: DEVELOPMENTS AND APPLICATIONS, 2016 *

Also Published As

Publication number Publication date
PL235163B1 (pl) 2020-06-01

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