PL424971A1 - Method for producing chitosan gels forming in the human body temperature, intended for injection scaffolds for breeding of nerve cells - Google Patents

Method for producing chitosan gels forming in the human body temperature, intended for injection scaffolds for breeding of nerve cells

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Publication number
PL424971A1
PL424971A1 PL424971A PL42497118A PL424971A1 PL 424971 A1 PL424971 A1 PL 424971A1 PL 424971 A PL424971 A PL 424971A PL 42497118 A PL42497118 A PL 42497118A PL 424971 A1 PL424971 A1 PL 424971A1
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PL
Poland
Prior art keywords
human body
scaffolds
chitosan
injection
intended
Prior art date
Application number
PL424971A
Other languages
Polish (pl)
Other versions
PL235369B1 (en
Inventor
Ireneusz Majsterek
Zofia MODRZEJEWSKA
Katarzyna Pieklarz
Michał Tylman
Original Assignee
Politechnika Łódzka
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Application filed by Politechnika Łódzka filed Critical Politechnika Łódzka
Priority to PL424971A priority Critical patent/PL235369B1/en
Publication of PL424971A1 publication Critical patent/PL424971A1/en
Publication of PL235369B1 publication Critical patent/PL235369B1/en

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Abstract

Przedmiotem zgłoszenia jest sposób wytwarzania żeli chitozanowych, formujących się w temperaturze ciała ludzkiego, przeznaczonych na skafoldy iniekcyjne do hodowli komórek nerwowych, który polega na wprowadzeniu do schłodzonego do temperatury 4°C roztworu wodnego octanu, chlorku, mleczanu chitozanu, sporządzonego z chitozanu o stopniu deacetylacji powyżej 70% i o masie cząsteczkowej 500 - 700 kDa, kroplami schłodzonego także do temperatury 4°C roztworu wodnego soli disodowej 5'-monofosforanu urydyny, zmieszaniu składników i odpowietrzeniu w temperaturze 4°C w czasie 24 godzin powstałego zolu przechodzącego w postać żelu w temperaturze ciała ludzkiego. Do roztworu soli chitozanu, przed wprowadzeniem roztworu soli 5'-monofosforanu urydyny, wprowadza się także środki wspomagające regenerację nerwów, jak kobalamina, kwas liponowy, tlenek grafenu.The subject of the application is a method for the production of chitosan gels, formed at the temperature of the human body, intended for injection scaffolds for the cultivation of nerve cells, which consists in introducing a cooled to 4 ° C aqueous solution of chitosan acetate, chloride, lactate, made of chitosan with a deacetylation degree above 70% and with a molecular weight of 500 - 700 kDa, dropwise, also cooled to a temperature of 4 ° C, an aqueous solution of uridine 5'-monophosphate disodium salt, mixing the components and deaerating at 4 ° C during 24 hours of the resulting sol transforming into a gel at a temperature of the human body. Nerve regeneration aids such as cobalamin, lipoic acid, graphene oxide are also introduced into the chitosan salt solution, prior to the introduction of the uridine 5'-monophosphate salt solution.

PL424971A 2018-03-20 2018-03-20 Method for producing chitosan gels forming in the human body temperature, intended for injection scaffolds for breeding of nerve cells PL235369B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL424971A PL235369B1 (en) 2018-03-20 2018-03-20 Method for producing chitosan gels forming in the human body temperature, intended for injection scaffolds for breeding of nerve cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL424971A PL235369B1 (en) 2018-03-20 2018-03-20 Method for producing chitosan gels forming in the human body temperature, intended for injection scaffolds for breeding of nerve cells

Publications (2)

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PL424971A1 true PL424971A1 (en) 2019-09-23
PL235369B1 PL235369B1 (en) 2020-06-29

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Family Applications (1)

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PL424971A PL235369B1 (en) 2018-03-20 2018-03-20 Method for producing chitosan gels forming in the human body temperature, intended for injection scaffolds for breeding of nerve cells

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL219576B1 (en) * 2010-11-22 2015-05-29 Politechnika Łódzka Process for the preparation of a temperature-sensitive chitosan hydrogel containing calcium and phosphorus
CN104983672A (en) * 2015-06-26 2015-10-21 青岛大学 Preparation method for temperature-sensitive sol

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL219576B1 (en) * 2010-11-22 2015-05-29 Politechnika Łódzka Process for the preparation of a temperature-sensitive chitosan hydrogel containing calcium and phosphorus
CN104983672A (en) * 2015-06-26 2015-10-21 青岛大学 Preparation method for temperature-sensitive sol

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZOFIA MODRZEJEWSKA, BIOLOGICAL PROPERTIES OF THERMOSENSITIVE CHITOSAN GELS CONDITIONING IN WATER, 2015 *

Also Published As

Publication number Publication date
PL235369B1 (en) 2020-06-29

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