PL422615A1 - Vaccine for porcine circovirus infection and method for obtaining it - Google Patents

Vaccine for porcine circovirus infection and method for obtaining it

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Publication number
PL422615A1
PL422615A1 PL422615A PL42261517A PL422615A1 PL 422615 A1 PL422615 A1 PL 422615A1 PL 422615 A PL422615 A PL 422615A PL 42261517 A PL42261517 A PL 42261517A PL 422615 A1 PL422615 A1 PL 422615A1
Authority
PL
Poland
Prior art keywords
seq
protein
amino acid
antigen
acid sequence
Prior art date
Application number
PL422615A
Other languages
Polish (pl)
Other versions
PL236294B1 (en
Inventor
Przemysław Grela
Piotr Koper
Justyna Kutkowska
Krzysztof Kwit
Andrzej Mazur
Zygmunt Pejsak
Andrzej Pochodyła
Katarzyna Podgórska
Andrzej Rapak
Anna Skorupska
Anna Szczotka-Bochniarz
Marek Tchórzewski
Kamil Żebracki
Original Assignee
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Uniwersytet Marii Curie-Skłodowskiej
Instytut Immunologii I Terapii Doświadczalnej Polskiej Akademii Nauk Im. Ludwika Hirszfelda We Wrocławiu
Vepharm Spółka Z Ograniczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy, Uniwersytet Marii Curie-Skłodowskiej, Instytut Immunologii I Terapii Doświadczalnej Polskiej Akademii Nauk Im. Ludwika Hirszfelda We Wrocławiu, Vepharm Spółka Z Ograniczoną Odpowiedzialnością filed Critical Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Priority to PL422615A priority Critical patent/PL236294B1/en
Publication of PL422615A1 publication Critical patent/PL422615A1/en
Publication of PL236294B1 publication Critical patent/PL236294B1/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest białko immunogenne lub zawarty w nim antygen, przy czym białko ma sekwencję aminokwasową białka CAP wirusa PCV2b o SEQ ID NO: 1, natomiast antygen jest peptydem o sekwencji aminokwasowej wybranej spośród: SEQ ID NO: 2-7, korzystnie SEQ ID NO: 2, 3 lub 5. Zgłoszenie zawiera też sposób otrzymywania białka immunogennego, znamienny tym, że obejmuje: a. ekspresję heterologiczną białka rekombinowanego w drożdżach Pichia pastoris, poprzez hodowlę komórek drożdży zawierających kasetę ekspresyjną kodującą białko o SEQ ID NO: 1, korzystnie zawierającą sekwencję kodującą o SEQ ID NO: 8, b. izolowanie rekombinowanego białka immunogennego o SEQ ID NO: 1 z płynu pohodowlanego. Ponadto, przedmiotem zgłoszenia jest szczepionka przeciwko zakażeniom cirkowirusowym świń zawierająca białko immunogenne o sekwencji SEQ ID NO: 1, 9 albo 10 lub antygen będący peptydem o sekwencji aminokwasowej wybranej spośród: SEQ ID NO: 2-7, zwłaszcza 2, 3 lub 5, korzystnie związany z farmaceutycznie dopuszczalnym nośnikiem, oraz adiuwant. Zgłoszenie obejmuje też zestaw immunodiagnostyczny do wykrywania przeciwciał przeciwko cirkowirusowi świń oraz zastosowanie białka immunogennego o sekwencji SEQ ID NO: 1, 9 lub 10 lub antygenu będącego peptydem o sekwencji aminokwasowej wybranej z pośród: SEQ ID NO: 2-7.The subject of the application is an immunogenic protein or antigen contained therein, wherein the protein has the amino acid sequence of the PCV2b CAP protein with SEQ ID NO: 1, while the antigen is a peptide with an amino acid sequence selected from: SEQ ID NO: 2-7, preferably SEQ ID NO : 2, 3 or 5. The application also contains a method for obtaining an immunogenic protein, characterized in that it comprises: a. Heterologous expression of recombinant protein in the yeast Pichia pastoris, by culturing yeast cells containing an expression cassette encoding a protein having SEQ ID NO: 1, preferably containing coding sequence of SEQ ID NO: 8, b. isolating the recombinant immunogenic protein of SEQ ID NO: 1 from the post-culture fluid. In addition, the subject of the application is a vaccine against porcine circovirus infection comprising an immunogenic protein with the sequence SEQ ID NO: 1, 9 or 10 or an antigen being a peptide with an amino acid sequence selected from: SEQ ID NO: 2-7, especially 2, 3 or 5, preferably associated with a pharmaceutically acceptable carrier, and an adjuvant. The application also includes an immunodiagnostic kit for detecting antibodies to porcine circovirus and the use of an immunogenic protein with the sequence SEQ ID NO: 1, 9 or 10 or an antigen which is a peptide with an amino acid sequence selected from among: SEQ ID NO: 2-7.

PL422615A 2017-08-22 2017-08-22 Vaccine for porcine circovirus infection and method for obtaining it PL236294B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL422615A PL236294B1 (en) 2017-08-22 2017-08-22 Vaccine for porcine circovirus infection and method for obtaining it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL422615A PL236294B1 (en) 2017-08-22 2017-08-22 Vaccine for porcine circovirus infection and method for obtaining it

Publications (2)

Publication Number Publication Date
PL422615A1 true PL422615A1 (en) 2019-02-25
PL236294B1 PL236294B1 (en) 2020-12-28

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ID=65431198

Family Applications (1)

Application Number Title Priority Date Filing Date
PL422615A PL236294B1 (en) 2017-08-22 2017-08-22 Vaccine for porcine circovirus infection and method for obtaining it

Country Status (1)

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PL (1) PL236294B1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103436499A (en) * 2013-06-09 2013-12-11 重庆澳龙生物制品有限公司 Porcine circovirus-like particle, and vaccine and preparation method thereof
CN103540605A (en) * 2013-09-22 2014-01-29 重庆市畜牧科学院 Capsid protein phage display particle of recombinant II porcine circovirus as well as preparation method and application thereof
CN106478783A (en) * 2015-08-24 2017-03-08 北京大北农科技集团股份有限公司动物医学研究中心 A kind of Porcine Circovirus genetic engineering subunit vaccine and its application

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103436499A (en) * 2013-06-09 2013-12-11 重庆澳龙生物制品有限公司 Porcine circovirus-like particle, and vaccine and preparation method thereof
CN103540605A (en) * 2013-09-22 2014-01-29 重庆市畜牧科学院 Capsid protein phage display particle of recombinant II porcine circovirus as well as preparation method and application thereof
CN106478783A (en) * 2015-08-24 2017-03-08 北京大北农科技集团股份有限公司动物医学研究中心 A kind of Porcine Circovirus genetic engineering subunit vaccine and its application

Also Published As

Publication number Publication date
PL236294B1 (en) 2020-12-28

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