PL422615A1 - Vaccine for porcine circovirus infection and method for obtaining it - Google Patents
Vaccine for porcine circovirus infection and method for obtaining itInfo
- Publication number
- PL422615A1 PL422615A1 PL422615A PL42261517A PL422615A1 PL 422615 A1 PL422615 A1 PL 422615A1 PL 422615 A PL422615 A PL 422615A PL 42261517 A PL42261517 A PL 42261517A PL 422615 A1 PL422615 A1 PL 422615A1
- Authority
- PL
- Poland
- Prior art keywords
- seq
- protein
- amino acid
- antigen
- acid sequence
- Prior art date
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia jest białko immunogenne lub zawarty w nim antygen, przy czym białko ma sekwencję aminokwasową białka CAP wirusa PCV2b o SEQ ID NO: 1, natomiast antygen jest peptydem o sekwencji aminokwasowej wybranej spośród: SEQ ID NO: 2-7, korzystnie SEQ ID NO: 2, 3 lub 5. Zgłoszenie zawiera też sposób otrzymywania białka immunogennego, znamienny tym, że obejmuje: a. ekspresję heterologiczną białka rekombinowanego w drożdżach Pichia pastoris, poprzez hodowlę komórek drożdży zawierających kasetę ekspresyjną kodującą białko o SEQ ID NO: 1, korzystnie zawierającą sekwencję kodującą o SEQ ID NO: 8, b. izolowanie rekombinowanego białka immunogennego o SEQ ID NO: 1 z płynu pohodowlanego. Ponadto, przedmiotem zgłoszenia jest szczepionka przeciwko zakażeniom cirkowirusowym świń zawierająca białko immunogenne o sekwencji SEQ ID NO: 1, 9 albo 10 lub antygen będący peptydem o sekwencji aminokwasowej wybranej spośród: SEQ ID NO: 2-7, zwłaszcza 2, 3 lub 5, korzystnie związany z farmaceutycznie dopuszczalnym nośnikiem, oraz adiuwant. Zgłoszenie obejmuje też zestaw immunodiagnostyczny do wykrywania przeciwciał przeciwko cirkowirusowi świń oraz zastosowanie białka immunogennego o sekwencji SEQ ID NO: 1, 9 lub 10 lub antygenu będącego peptydem o sekwencji aminokwasowej wybranej z pośród: SEQ ID NO: 2-7.The subject of the application is an immunogenic protein or antigen contained therein, wherein the protein has the amino acid sequence of the PCV2b CAP protein with SEQ ID NO: 1, while the antigen is a peptide with an amino acid sequence selected from: SEQ ID NO: 2-7, preferably SEQ ID NO : 2, 3 or 5. The application also contains a method for obtaining an immunogenic protein, characterized in that it comprises: a. Heterologous expression of recombinant protein in the yeast Pichia pastoris, by culturing yeast cells containing an expression cassette encoding a protein having SEQ ID NO: 1, preferably containing coding sequence of SEQ ID NO: 8, b. isolating the recombinant immunogenic protein of SEQ ID NO: 1 from the post-culture fluid. In addition, the subject of the application is a vaccine against porcine circovirus infection comprising an immunogenic protein with the sequence SEQ ID NO: 1, 9 or 10 or an antigen being a peptide with an amino acid sequence selected from: SEQ ID NO: 2-7, especially 2, 3 or 5, preferably associated with a pharmaceutically acceptable carrier, and an adjuvant. The application also includes an immunodiagnostic kit for detecting antibodies to porcine circovirus and the use of an immunogenic protein with the sequence SEQ ID NO: 1, 9 or 10 or an antigen which is a peptide with an amino acid sequence selected from among: SEQ ID NO: 2-7.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL422615A PL236294B1 (en) | 2017-08-22 | 2017-08-22 | Vaccine for porcine circovirus infection and method for obtaining it |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL422615A PL236294B1 (en) | 2017-08-22 | 2017-08-22 | Vaccine for porcine circovirus infection and method for obtaining it |
Publications (2)
Publication Number | Publication Date |
---|---|
PL422615A1 true PL422615A1 (en) | 2019-02-25 |
PL236294B1 PL236294B1 (en) | 2020-12-28 |
Family
ID=65431198
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL422615A PL236294B1 (en) | 2017-08-22 | 2017-08-22 | Vaccine for porcine circovirus infection and method for obtaining it |
Country Status (1)
Country | Link |
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PL (1) | PL236294B1 (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103436499A (en) * | 2013-06-09 | 2013-12-11 | 重庆澳龙生物制品有限公司 | Porcine circovirus-like particle, and vaccine and preparation method thereof |
CN103540605A (en) * | 2013-09-22 | 2014-01-29 | 重庆市畜牧科学院 | Capsid protein phage display particle of recombinant II porcine circovirus as well as preparation method and application thereof |
CN106478783A (en) * | 2015-08-24 | 2017-03-08 | 北京大北农科技集团股份有限公司动物医学研究中心 | A kind of Porcine Circovirus genetic engineering subunit vaccine and its application |
-
2017
- 2017-08-22 PL PL422615A patent/PL236294B1/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103436499A (en) * | 2013-06-09 | 2013-12-11 | 重庆澳龙生物制品有限公司 | Porcine circovirus-like particle, and vaccine and preparation method thereof |
CN103540605A (en) * | 2013-09-22 | 2014-01-29 | 重庆市畜牧科学院 | Capsid protein phage display particle of recombinant II porcine circovirus as well as preparation method and application thereof |
CN106478783A (en) * | 2015-08-24 | 2017-03-08 | 北京大北农科技集团股份有限公司动物医学研究中心 | A kind of Porcine Circovirus genetic engineering subunit vaccine and its application |
Also Published As
Publication number | Publication date |
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PL236294B1 (en) | 2020-12-28 |
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