PL415670A1 - Method for immobilizing microorganisms on and/or in bacterial cellulose - Google Patents
Method for immobilizing microorganisms on and/or in bacterial celluloseInfo
- Publication number
- PL415670A1 PL415670A1 PL415670A PL41567016A PL415670A1 PL 415670 A1 PL415670 A1 PL 415670A1 PL 415670 A PL415670 A PL 415670A PL 41567016 A PL41567016 A PL 41567016A PL 415670 A1 PL415670 A1 PL 415670A1
- Authority
- PL
- Poland
- Prior art keywords
- bacteria
- bacterial cellulose
- rpm
- suspension
- lactobacillus spp
- Prior art date
Links
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób immobilizacji mikroorganizmów na i/lub w celulozie bakteryjnej, który charakteryzuje się tym, że mokrą lub suchą celulozę bakteryjną umieszcza się w zawiesinie bakterii Lactobacillus spp., o gęstości 1° McFerland i inkubuje się ją w tej zawiesinie przez 24 h w temperaturze 25°C z wytrząsaniem 180 obr/min. W sposobie stosuje się bakterie Lactobacillus spp. podchodzące z 24 h hodowli prowadzonej w płynnym podłożu MRS (deMan, Rogosa and Sharpe), które oddzielono od podłoża poprzez wirowanie przez 15 minut przy 3000 obr/minutę. Zawiesinę bakterii Lactobacillus spp. otrzymano umieszczając bakterie w buforze fosforanowym (PBS). Do immobilizacji bakterii Lactbacillus spp. można zastosować celulozę bakteryjną w postaci membran lub kulek, uzyskaną w wyniku prowadzenia 6 dniowej hodowli, odpowiednio, w warunkach stacjonarnych lub wytrząsanych przy zastosowaniu 180 obr/min.The subject of the application is a method of immobilizing microorganisms on and / or in bacterial cellulose, which is characterized in that wet or dry bacterial cellulose is placed in a suspension of Lactobacillus spp. Bacteria with a density of 1 ° McFerland and incubated in this suspension for 24 h at temperature 25 ° C with shaking 180 rpm. The method uses Lactobacillus spp. Bacteria coming from a 24-hour culture in a liquid MRS medium (deMan, Rogosa and Sharpe), which were separated from the medium by centrifugation for 15 minutes at 3000 rpm. The suspension of Lactobacillus spp. Bacteria was obtained by placing the bacteria in phosphate buffer (PBS). For the immobilization of Lactbacillus spp. Bacteria, bacterial cellulose in the form of membranes or beads can be used, obtained as a result of 6-day cultivation, respectively, under stationary or shaken conditions using 180 rpm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL415670A PL234248B1 (en) | 2016-01-08 | 2016-01-08 | Method for immobilizing microorganisms on and/or in bacterial cellulose |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL415670A PL234248B1 (en) | 2016-01-08 | 2016-01-08 | Method for immobilizing microorganisms on and/or in bacterial cellulose |
Publications (2)
Publication Number | Publication Date |
---|---|
PL415670A1 true PL415670A1 (en) | 2017-07-17 |
PL234248B1 PL234248B1 (en) | 2020-01-31 |
Family
ID=59298046
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL415670A PL234248B1 (en) | 2016-01-08 | 2016-01-08 | Method for immobilizing microorganisms on and/or in bacterial cellulose |
Country Status (1)
Country | Link |
---|---|
PL (1) | PL234248B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021009038A1 (en) | 2019-07-12 | 2021-01-21 | Evonik Operations Gmbh | Method for loading of microorganisms on multiphase biomaterials |
-
2016
- 2016-01-08 PL PL415670A patent/PL234248B1/en unknown
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021009038A1 (en) | 2019-07-12 | 2021-01-21 | Evonik Operations Gmbh | Method for loading of microorganisms on multiphase biomaterials |
WO2021009021A1 (en) | 2019-07-12 | 2021-01-21 | Evonik Operations Gmbh | Method for loading of microorganisms on multiphase biomaterials |
Also Published As
Publication number | Publication date |
---|---|
PL234248B1 (en) | 2020-01-31 |
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