PL415670A1 - Method for immobilizing microorganisms on and/or in bacterial cellulose - Google Patents

Method for immobilizing microorganisms on and/or in bacterial cellulose

Info

Publication number
PL415670A1
PL415670A1 PL415670A PL41567016A PL415670A1 PL 415670 A1 PL415670 A1 PL 415670A1 PL 415670 A PL415670 A PL 415670A PL 41567016 A PL41567016 A PL 41567016A PL 415670 A1 PL415670 A1 PL 415670A1
Authority
PL
Poland
Prior art keywords
bacteria
bacterial cellulose
rpm
suspension
lactobacillus spp
Prior art date
Application number
PL415670A
Other languages
Polish (pl)
Other versions
PL234248B1 (en
Inventor
Karol Fijałkowski
Dorota Peitler
Anna Żywicka
Rafał Rakoczy
Original Assignee
Zachodniopomorski Uniwersytet Technologiczny W Szczecinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zachodniopomorski Uniwersytet Technologiczny W Szczecinie filed Critical Zachodniopomorski Uniwersytet Technologiczny W Szczecinie
Priority to PL415670A priority Critical patent/PL234248B1/en
Publication of PL415670A1 publication Critical patent/PL415670A1/en
Publication of PL234248B1 publication Critical patent/PL234248B1/en

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacji mikroorganizmów na i/lub w celulozie bakteryjnej, który charakteryzuje się tym, że mokrą lub suchą celulozę bakteryjną umieszcza się w zawiesinie bakterii Lactobacillus spp., o gęstości 1° McFerland i inkubuje się ją w tej zawiesinie przez 24 h w temperaturze 25°C z wytrząsaniem 180 obr/min. W sposobie stosuje się bakterie Lactobacillus spp. podchodzące z 24 h hodowli prowadzonej w płynnym podłożu MRS (deMan, Rogosa and Sharpe), które oddzielono od podłoża poprzez wirowanie przez 15 minut przy 3000 obr/minutę. Zawiesinę bakterii Lactobacillus spp. otrzymano umieszczając bakterie w buforze fosforanowym (PBS). Do immobilizacji bakterii Lactbacillus spp. można zastosować celulozę bakteryjną w postaci membran lub kulek, uzyskaną w wyniku prowadzenia 6 dniowej hodowli, odpowiednio, w warunkach stacjonarnych lub wytrząsanych przy zastosowaniu 180 obr/min.The subject of the application is a method of immobilizing microorganisms on and / or in bacterial cellulose, which is characterized in that wet or dry bacterial cellulose is placed in a suspension of Lactobacillus spp. Bacteria with a density of 1 ° McFerland and incubated in this suspension for 24 h at temperature 25 ° C with shaking 180 rpm. The method uses Lactobacillus spp. Bacteria coming from a 24-hour culture in a liquid MRS medium (deMan, Rogosa and Sharpe), which were separated from the medium by centrifugation for 15 minutes at 3000 rpm. The suspension of Lactobacillus spp. Bacteria was obtained by placing the bacteria in phosphate buffer (PBS). For the immobilization of Lactbacillus spp. Bacteria, bacterial cellulose in the form of membranes or beads can be used, obtained as a result of 6-day cultivation, respectively, under stationary or shaken conditions using 180 rpm.

PL415670A 2016-01-08 2016-01-08 Method for immobilizing microorganisms on and/or in bacterial cellulose PL234248B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL415670A PL234248B1 (en) 2016-01-08 2016-01-08 Method for immobilizing microorganisms on and/or in bacterial cellulose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL415670A PL234248B1 (en) 2016-01-08 2016-01-08 Method for immobilizing microorganisms on and/or in bacterial cellulose

Publications (2)

Publication Number Publication Date
PL415670A1 true PL415670A1 (en) 2017-07-17
PL234248B1 PL234248B1 (en) 2020-01-31

Family

ID=59298046

Family Applications (1)

Application Number Title Priority Date Filing Date
PL415670A PL234248B1 (en) 2016-01-08 2016-01-08 Method for immobilizing microorganisms on and/or in bacterial cellulose

Country Status (1)

Country Link
PL (1) PL234248B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021009038A1 (en) 2019-07-12 2021-01-21 Evonik Operations Gmbh Method for loading of microorganisms on multiphase biomaterials

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021009038A1 (en) 2019-07-12 2021-01-21 Evonik Operations Gmbh Method for loading of microorganisms on multiphase biomaterials
WO2021009021A1 (en) 2019-07-12 2021-01-21 Evonik Operations Gmbh Method for loading of microorganisms on multiphase biomaterials

Also Published As

Publication number Publication date
PL234248B1 (en) 2020-01-31

Similar Documents

Publication Publication Date Title
RU2021130638A (en) USE OF MICROBIAL COMMUNITIES FOR HUMAN AND ANIMAL TREATMENT
MX2019003962A (en) Method for culturing bacillus bacterium, and method for producing useful substance.
MX2021006546A (en) Use of lactase to provide high gos fiber level and low lactose level.
WO2016181228A3 (en) Systems and methods for growing a biofilm of probiotic bacteria on solid particles for colonization of bacteria in the gut
NZ721752A (en) Method for preparing fermentation broth extracts having chymosin activity and products thereof
CN105062957A (en) Culture method of vascular endothelial progenitor cells
MX2020011318A (en) Microbiological growth media and methods of using the same.
BR112017003556A2 (en) method of cultivation of a microorganism.
AR059863A1 (en) LACTOBACILLUS HELVETICUS CEPAS
PL415670A1 (en) Method for immobilizing microorganisms on and/or in bacterial cellulose
RU2019104079A (en) CJLM119 LEUCONOSTOC MESENTEROIDES STRAIN, PRODUCING REDUCED GAS QUANTITY, AND METHOD FOR PREPARING KIMCHI USING THIS STRAIN
Bi et al. Migration and retention mechanism of microorganisms for oil recovery in porous media
Zgair et al. Adhering ability of Stenotrophomonas maltophilia is dependent on growth conditions
WO2015193811A3 (en) Production of 1-propanol
SG10201703032YA (en) Product
EA201890581A1 (en) SYSTEMS, METHODS AND DEVICES FOR INCREASING THE CAPACITY OF A BIOREACTOR USING POLYMERS BASED ON SILICON DIOXIDE
MX2019014556A (en) Low-agglomeration, enzyme-containing particles.
PL415673A1 (en) Method for producing bacterial cellulose with immobilised microorganisms
CheMan et al. Effect of the cross linkers' concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase).
UA123122U (en) METHOD OF OBTAINING THE METABOLITES OF PROBIOTIC BACTERIA Strains
NZ742855A (en) Assay for determining antibiotics in waste
MY197963A (en) Algae treatment method
RU2012110981A (en) TEST SYSTEM FOR IN-NATURAL AND IN-SPECIFIC DIFFERENTIATION OF BACTERIA OF THE GENUS YERSINIA
Bazukyan et al. SOME PROPERTIES OF ANTIBACTERIAL COMPONENT OF LACTIC ACID BACTERIA ISOLATED FROM ARMENIAN DAIRY PRODUCTS.
TR201807746A2 (en) NEW GENERATION OF FERMENTED DAIRY PRODUCTS