PL415673A1 - Method for producing bacterial cellulose with immobilised microorganisms - Google Patents
Method for producing bacterial cellulose with immobilised microorganismsInfo
- Publication number
- PL415673A1 PL415673A1 PL415673A PL41567316A PL415673A1 PL 415673 A1 PL415673 A1 PL 415673A1 PL 415673 A PL415673 A PL 415673A PL 41567316 A PL41567316 A PL 41567316A PL 415673 A1 PL415673 A1 PL 415673A1
- Authority
- PL
- Poland
- Prior art keywords
- bacteria
- lactbacillus
- suspension
- medium
- bacterial cellulose
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób wytwarzania celulozy bakteryjnej, który charakteryzuje się tym, że wraz z bakterią Gluconacetobacter xylinus na podłoże Herstina-Schramma zaszczepia się zawiesinę bakterii probiotacznych z rodzaju Lactbacillus o gęstości 0,5° McFerland (1,5 • 108 CFU/mL). W sposobie stosuje się zawiesinę bakterii Lactbacillus otrzymaną z 24 h hodowli prowadzonej w płynnym podłożu MRS (deMan, Rogosa and Sharpe). Bakterie te oddziela się od podłoża poprzez odwirowanie z prędkością 3000 obr/min przez 15 min, a następnie inkubuje w buforze fosforanowym (PBS), w celu otrzymania zawiesiny komórek o określonej gęstości. Stosunek objętościowy bakterii G. xylinus i mikroorganizmów probiotycznych z rodzaju Lactbacillus wynosi 1 do 100. Hodowlę tych bakterii można prowadzić metodą stacjonarną lub wytrząsaną przez okres 6 dni, utrzymując temperaturę w zakresie 28 - 30°C.The subject of the application is a method for producing bacterial cellulose, which is characterized in that, together with the bacterium Gluconacetobacter xylinus, a suspension of probiotic bacteria of the genus Lactbacillus with a density of 0.5 ° McFerland (1.5 • 108 CFU / mL) is inoculated onto the Herstin-Schramma medium. The method uses a suspension of Lactbacillus bacteria obtained from 24 h cultivated in liquid MRS medium (deMan, Rogosa and Sharpe). These bacteria are separated from the medium by centrifugation at 3000 rpm for 15 min and then incubated in phosphate buffer (PBS) to obtain a cell density suspension of specified density. The volume ratio of G. xylinus bacteria and probiotic microorganisms of the genus Lactbacillus is 1 to 100. The cultivation of these bacteria can be carried out by a stationary or shaken method for a period of 6 days, maintaining the temperature in the range of 28-30 ° C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415673A PL415673A1 (en) | 2016-01-08 | 2016-01-08 | Method for producing bacterial cellulose with immobilised microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415673A PL415673A1 (en) | 2016-01-08 | 2016-01-08 | Method for producing bacterial cellulose with immobilised microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL415673A1 true PL415673A1 (en) | 2017-07-17 |
Family
ID=59298041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL415673A PL415673A1 (en) | 2016-01-08 | 2016-01-08 | Method for producing bacterial cellulose with immobilised microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL415673A1 (en) |
-
2016
- 2016-01-08 PL PL415673A patent/PL415673A1/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mahrous et al. | Study bacteriocin production and optimization using new isolates of Lactobacillus spp. isolated from some dairy products under different culture conditions | |
| MX2019003962A (en) | Method for culturing bacillus bacterium, and method for producing useful substance. | |
| HK1135849A1 (en) | A method for mite production | |
| NZ627915A (en) | Probiotic derived non-viable material for infection prevention and treatment | |
| MX2018002278A (en) | Lactobacillus fermentum bacteria with antifungal activity. | |
| MX2018002283A (en) | Lactobacillus fermentum bacteria inhibiting post-acidification. | |
| MX2011006432A (en) | Method for controlling streptococcus pneumoniae serotype 19a polysaccharide molecular weight. | |
| RU2014143979A (en) | SUITABLE FOR USE IN FOOD BACTERIA LACTOBACILLUS RHAMNOSUS | |
| Jagadeeswari et al. | Isolation and characterization of bacteriocin producing Lactobacillus sp. from traditional fermented foods. | |
| PH12019550231A1 (en) | Probiotic bacteria preconditioned in a gos-containing medium and use thereof | |
| Mesomo et al. | Xanthan gum produced by Xanthomonas campestris from cheese whey: production optimisation and rheological characterisation | |
| JP2020528264A5 (en) | ||
| Kumar Bajaj et al. | Physico-chemical characterization of exopolysaccharides of potential probiotic Enterococcus faecium isolates from infants’ gut | |
| Bevilacqua et al. | Combined effects of pH, yeast extract, carbohydrates and di-ammonium hydrogen citrate on the biomass production and acidifying ability of a probiotic Lactobacillus plantarum strain, isolated from table olives, in a batch system | |
| RU2019104079A (en) | CJLM119 LEUCONOSTOC MESENTEROIDES STRAIN, PRODUCING REDUCED GAS QUANTITY, AND METHOD FOR PREPARING KIMCHI USING THIS STRAIN | |
| Grosu-Tudor et al. | Exopolysaccharide production by selected lactic acid bacteria isolated from fermented vegetables. | |
| WO2018183848A8 (en) | Media for microorganism culture and related compositions and methods | |
| RU2015129989A (en) | A method of obtaining a probiotic composition | |
| PL415673A1 (en) | Method for producing bacterial cellulose with immobilised microorganisms | |
| Darmasiwi et al. | Edible biofilm formation from guava seed waste fermentation | |
| Shu et al. | Effect of bile and nalidixic acid on growth of selected probiotics | |
| Babu et al. | Extraction of prodigiosin from Serratia marcescens and its application as an antibacterial spray | |
| Ferdes et al. | The influence of the carbon source on torularhodin pigment biosynthesis | |
| MX2020003295A (en) | Production method for bacterial cells. | |
| MD865Z (en) | Process for producing a powdery fermented dairy product |