PL415291A1 - Method for obtaining a transplant for regeneration of bone tissue, preferably human and the transplant for regeneration of bone tissue, preferably human - Google Patents
Method for obtaining a transplant for regeneration of bone tissue, preferably human and the transplant for regeneration of bone tissue, preferably humanInfo
- Publication number
- PL415291A1 PL415291A1 PL415291A PL41529115A PL415291A1 PL 415291 A1 PL415291 A1 PL 415291A1 PL 415291 A PL415291 A PL 415291A PL 41529115 A PL41529115 A PL 41529115A PL 415291 A1 PL415291 A1 PL 415291A1
- Authority
- PL
- Poland
- Prior art keywords
- bone
- concentration
- regeneration
- bone tissue
- nacl
- Prior art date
Links
- 210000000988 bone and bone Anatomy 0.000 title abstract 14
- 230000008929 regeneration Effects 0.000 title abstract 3
- 238000011069 regeneration method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 8
- 239000008187 granular material Substances 0.000 abstract 6
- 239000011780 sodium chloride Substances 0.000 abstract 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 3
- 235000019441 ethanol Nutrition 0.000 abstract 3
- 238000004140 cleaning Methods 0.000 abstract 2
- 238000005238 degreasing Methods 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 230000008014 freezing Effects 0.000 abstract 2
- 238000007710 freezing Methods 0.000 abstract 2
- 230000005855 radiation Effects 0.000 abstract 2
- 210000004872 soft tissue Anatomy 0.000 abstract 2
- 230000001054 cortical effect Effects 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 230000017423 tissue regeneration Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
Sposób otrzymywania przeszczepu do regeneracji tkanki kostnej, zwłaszcza ludzkiej, charakteryzuje się tym, że składa się z następujących po sobie etapów: oczyszczania mechanicznego kości zbitej, gąbczastej lub korowo - gąbczastej, korzystnie pochodzenia ludzkiego z tkanek miękkich, dzielenia kości na fragmenty, korzystnie odłuszczania kości w C2H5OH o stężeniu od 60% do 96%, odtłuszczania kości w H2O2 o stężeniu od 1% do 40%, co najmniej jednokrotnego płukania kości w NaCl o stężeniu od 0,1% do 10%, doczyszczania mechanicznego kości, odsączania kości z resztek NaCl, zamrażania kości w temp. około -40°C, mielenia kości młynem mechanicznym, aż do uzyskania granulatu, przesiewania granulatu przez system sit, w celu podziału na odpowiednie frakcje, korzystnie zamrażania granulatu w temp. około -40°C i sterylizacji radiacyjnej granulatu, korzystnie w dawce 35 kGy. Przeszczep do regeneracji tkanki kostnej, zwłaszcza ludzkiej, charakteryzuje się tym, że stanowi go tkanka kostna zbita, korowo - gąbczasta lub gąbczasta, korzystnie pochodzenia ludzkiego, w postaci granulatu, oczyszczona mechanicznie z tkanek miękkich, dzielona na fragmenty, korzystnie odłuszczana w C2H5OH o stężeniu od 60% do 96%, odtłuszczana w H2O2 o stężeniu od 1% do 40%, co najmniej jednokrotnie płukana w NaCl o stężeniu od 0,1% do 10%, doczyszczona mechanicznie, pozbawiona resztek NaCl, H2O2, korzystnie również C2H5OH, zamrożona w temp. około -40°C, mielona aż do uzyskania granulatu, przesiana przez system sit, w celu podziału na odpowiednie frakcje, korzystnie ponownie zamrożona w temp. około -40°C i sterylizowana radiacyjnie, korzystnie w dawce 35 kGy.The method of obtaining a graft for the regeneration of bone tissue, especially human, is characterized by the fact that it consists of the following stages: mechanical cleaning of compacted, spongy or cortico-spongy bone, preferably of human origin from soft tissues, division of bone into fragments, preferably degreasing the bone in C2H5OH at a concentration of 60% to 96%, degreasing bone in H2O2 at a concentration of 1% to 40%, at least once washing the bone in NaCl at a concentration of 0.1% to 10%, mechanical cleaning of bone, draining bone from debris NaCl, freezing bones at about -40 ° C, grinding the bones with a mechanical mill until obtaining granulate, sieving the granulate through a sieve system, to divide into appropriate fractions, preferably freezing the granulate at about -40 ° C and radiation sterilization granules, preferably at a dose of 35 kGy. A graft for bone tissue regeneration, especially of human, is characterized by the fact that it is a compact, cortical - spongy or spongy bone tissue, preferably of human origin, in the form of granules, mechanically purified from soft tissues, divided into fragments, preferably degreased in C2H5OH concentration 60% to 96%, degreased in H2O2 at a concentration of 1% to 40%, rinsed at least once in NaCl at a concentration of 0.1% to 10%, mechanically cleaned, free of residual NaCl, H2O2, preferably also C2H5OH, frozen at about -40 ° C, ground until granules are obtained, sieved through a sieve system, to divide into appropriate fractions, preferably re-frozen at about -40 ° C and radiation sterilized, preferably at a dose of 35 kGy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415291A PL232501B1 (en) | 2015-12-14 | 2015-12-14 | Method for obtaining a transplant for regeneration of bone tissue, preferably human and the transplant for regeneration of bone tissue, preferably human |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL415291A PL232501B1 (en) | 2015-12-14 | 2015-12-14 | Method for obtaining a transplant for regeneration of bone tissue, preferably human and the transplant for regeneration of bone tissue, preferably human |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL415291A1 true PL415291A1 (en) | 2017-06-19 |
| PL232501B1 PL232501B1 (en) | 2019-06-28 |
Family
ID=59061472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL415291A PL232501B1 (en) | 2015-12-14 | 2015-12-14 | Method for obtaining a transplant for regeneration of bone tissue, preferably human and the transplant for regeneration of bone tissue, preferably human |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL232501B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL248549B1 (en) * | 2022-05-18 | 2025-12-22 | Henryk Bursig | Method for obtaining a spatial bone graft with a defined shape |
-
2015
- 2015-12-14 PL PL415291A patent/PL232501B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL232501B1 (en) | 2019-06-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cui et al. | Characterisation of matrix vesicles in skeletal and soft tissue mineralisation | |
| Calori et al. | Treatment of AVN using the induction chamber technique and a biological-based approach: indications and clinical results | |
| MX359206B (en) | Microbial-based waste water treatment compositions and methods of use thereof. | |
| NZ597419A (en) | Formulations for the treatment of deep tissue pain | |
| KR20100047412A (en) | Manufacturing method for demineralized bone matrix | |
| EA201890291A1 (en) | GREAT THREE-DIMENSIONAL FRAMEWORKS, PERFORMED FROM ACTIVE HYDROXYAPATITIS, OBTAINED BY BIOMORPH TRANSFORMATION OF NATURAL STRUCTURES, AND METHOD OF THEIR RECEIVING | |
| MX2020005794A (en) | METHODS AND COMPOSITIONS TO AVOID ISCHEMIA-REPERFUSION INJURY IN ORGANS. | |
| WO2016067115A3 (en) | Cartilage prosthetic implant | |
| PL415287A1 (en) | Method for obtaining a transplant of cortical spongy bone debris for regeneration of bone tissue, and the transplant for this regeneration | |
| EP4069267A4 (en) | Histatin-1 formulation for the treatment, repair or regeneration of bone tissue in a subject | |
| PL441220A1 (en) | Method of obtaining a spatial bone graft of a defined shape, and spatial bone graft | |
| RU2009138989A (en) | METHOD FOR TREATING SQUILE FRACTURES OF TUBULAR BONES | |
| RU2614095C1 (en) | Method for fibula autoplastic properties optimisation | |
| Hwang et al. | Biomechanical Effect of Implantation of Chitosan/MWNTs Reinforced Scaffold into Damaged Femur | |
| Kumar | Venous thromboembolism in major lower limb orthopedic surgery | |
| Buyanov et al. | Effect of the osseointegration of hydrogel based on polyacrylamide and cellulose observed during in vivo experiments | |
| WEI et al. | Observation of curative effect of avulsion fracture of anterolateral ligament of knee joint | |
| PL415721A1 (en) | Method for increasing proteolytic activity in a bath remaining after marinating herrings | |
| HUANG et al. | Ilizarov technique for the treatment of femoral infectious ununtied fracture and bone defect | |
| RU2015147635A (en) | METHOD FOR TWO-JOINT ARTICLE ARTHRODESIS OF THE ANKI AND TALAN-AND-HEEL JOINT | |
| Vijay et al. | Comment on Zeng et al.: Vascularised greater trochanter bone graft, combined free iliac flap and impaction bone grafting for osteonecrosis of the femoral head | |
| PL232501B1 (en) | Method for obtaining a transplant for regeneration of bone tissue, preferably human and the transplant for regeneration of bone tissue, preferably human | |
| Sohoni et al. | The effects of a new preservation method on the biomechanics and shelf life of allograft bone | |
| YIN et al. | Cancellous bone wrapping grafting for segmental defects of long bone | |
| RU2010147960A (en) | METHOD OF VERTEBROPLASTIC TURNING BREAKING BODIES OF CALLS |