PL347223A1 - Nucleic acid molecules which code a branching enzyme from bacteria of the genus neisseria, and a method for producing α-1,6-branched α-1,4-glucans - Google Patents

Nucleic acid molecules which code a branching enzyme from bacteria of the genus neisseria, and a method for producing α-1,6-branched α-1,4-glucans

Info

Publication number
PL347223A1
PL347223A1 PL99347223A PL34722399A PL347223A1 PL 347223 A1 PL347223 A1 PL 347223A1 PL 99347223 A PL99347223 A PL 99347223A PL 34722399 A PL34722399 A PL 34722399A PL 347223 A1 PL347223 A1 PL 347223A1
Authority
PL
Poland
Prior art keywords
branching enzyme
glucans
bacteria
nucleic acid
branched
Prior art date
Application number
PL99347223A
Inventor
Volker Buettcher
Martin Quanz
Original Assignee
Planttec Biotechnologie Gmbh
Max Planck Gesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19846635A external-priority patent/DE19846635A1/en
Priority claimed from DE19924342A external-priority patent/DE19924342A1/en
Application filed by Planttec Biotechnologie Gmbh, Max Planck Gesellschaft filed Critical Planttec Biotechnologie Gmbh
Publication of PL347223A1 publication Critical patent/PL347223A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/22—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Neisseriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A nucleic acid (I) isolated from Neisseria encodes a branching enzyme (II). (I) has a fully defined 2475 bp sequence (given in the specification and encodes; (i) a protein with a fully defined 762 amino acid sequence (given in the specification); or (ii) a protein encoded by the insert in plasmid DSM 12425. Independent claims are also included for the following: (1) a vector containing (I); (2) host cells transformed with (I) or the vector in (1); (3) production of (II) by in vitro transcription and translation of (I); (4) antibodies (Ab) against (II); (5) transgenic plant cell containing (I), linked to regulatory sequences functional in plants; (6) transgenic plants containing the cells of (5) and their harvestable parts; (7) production of the plants of (h); (8) native regulatory region (RR) that controls transcription of (I) in bacteria; and (9) in vitro production of alpha -1,6-branched alpha -1,4-glucans (III) using sucrose as substrate and a combination of an amylosucrase and a branching enzyme (BE).
PL99347223A 1998-10-09 1999-10-08 Nucleic acid molecules which code a branching enzyme from bacteria of the genus neisseria, and a method for producing α-1,6-branched α-1,4-glucans PL347223A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19846635A DE19846635A1 (en) 1998-10-09 1998-10-09 New nucleic acid encoding a branching enzyme, useful for in vitro synthesis of branched glucans and to prepare transgenic plants producing modified starch
DE19924342A DE19924342A1 (en) 1999-05-27 1999-05-27 Genetically modified plant cells and plants with increased activity of an amylosucrase protein and a branching enzyme

Publications (1)

Publication Number Publication Date
PL347223A1 true PL347223A1 (en) 2002-03-25

Family

ID=26049420

Family Applications (1)

Application Number Title Priority Date Filing Date
PL99347223A PL347223A1 (en) 1998-10-09 1999-10-08 Nucleic acid molecules which code a branching enzyme from bacteria of the genus neisseria, and a method for producing α-1,6-branched α-1,4-glucans

Country Status (16)

Country Link
US (9) US6699694B1 (en)
EP (2) EP1117802B1 (en)
JP (1) JP2002527068A (en)
KR (1) KR20010099681A (en)
CN (1) CN1325449A (en)
AT (1) ATE455173T1 (en)
AU (1) AU765131C (en)
BR (1) BR9915026A (en)
CA (1) CA2345904A1 (en)
CZ (1) CZ20011125A3 (en)
DE (1) DE59915126D1 (en)
DK (1) DK1117802T3 (en)
ES (1) ES2339619T3 (en)
HU (1) HUP0104892A2 (en)
PL (1) PL347223A1 (en)
WO (1) WO2000022140A1 (en)

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