PL239291B1 - Method of preparing media for cultivating of water macrophytes from the Lemnaceae family - Google Patents
Method of preparing media for cultivating of water macrophytes from the Lemnaceae family Download PDFInfo
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- PL239291B1 PL239291B1 PL427546A PL42754618A PL239291B1 PL 239291 B1 PL239291 B1 PL 239291B1 PL 427546 A PL427546 A PL 427546A PL 42754618 A PL42754618 A PL 42754618A PL 239291 B1 PL239291 B1 PL 239291B1
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- lemnaceae
- macrophytes
- substrate
- ozonation
- water
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- 238000000034 method Methods 0.000 title claims description 21
- 241000209524 Araceae Species 0.000 title claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title description 4
- 239000000758 substrate Substances 0.000 claims description 12
- 238000006385 ozonation reaction Methods 0.000 claims description 11
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 description 14
- 241000196324 Embryophyta Species 0.000 description 12
- 240000000263 Lemna trisulca Species 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 239000002028 Biomass Substances 0.000 description 6
- 230000009469 supplementation Effects 0.000 description 5
- 244000207747 Lemna gibba Species 0.000 description 4
- 235000006438 Lemna gibba Nutrition 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- 241000209499 Lemna Species 0.000 description 3
- 239000003415 peat Substances 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- HYVABZIGRDEKCD-UHFFFAOYSA-N N(6)-dimethylallyladenine Chemical compound CC(C)=CCNC1=NC=NC2=C1N=CN2 HYVABZIGRDEKCD-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 240000005010 Landoltia punctata Species 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000195887 Physcomitrella patens Species 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000339989 Wolffia Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229960002413 ferric citrate Drugs 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000003966 growth inhibitor Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- -1 iron ions Chemical class 0.000 description 1
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 1
- 230000003050 macronutrient Effects 0.000 description 1
- 235000021073 macronutrients Nutrition 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000007003 mineral medium Substances 0.000 description 1
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical compound [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229940095064 tartrate Drugs 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/78—Treatment of water, waste water, or sewage by oxidation with ozone
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Botany (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hydrology & Water Resources (AREA)
- Ecology (AREA)
- Environmental & Geological Engineering (AREA)
- Cell Biology (AREA)
- Environmental Sciences (AREA)
- Forests & Forestry (AREA)
- Microbiology (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Treatment Of Water By Oxidation Or Reduction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
Przedmiotem wynalazku jest sposób przygotowania mediów do hodowli makrofitów wodnych z rodziny Lemnaceae,The subject of the invention is a method of preparing media for the cultivation of aquatic macrophytes from the Lemnaceae family,
Makrofity wodne z rodziny Lemnaceae z uwagi na wysoką zawartość białka stanowią potencjalne źródło pożywienia dla ludzi i zwierząt. Rośliny te znalazły również wiele zastosowań, przede wszystkim jako maty filtrujące z uwagi: na swoje zdolności fitoremediacyjne. Rośliny te stosowane są na całym świecie, w tym również w Polsce, do biologicznego oczyszczania wód i ścieków, a także ostatnio jako najnowsze źródło biomasy energetycznej do produkcji bioetanolu.Aquatic macrophytes from the Lemnaceae family, due to their high protein content, are a potential source of food for humans and animals. These plants have also found many uses, primarily as filter mats due to their phytoremediation abilities. These plants are used all over the world, including Poland, for biological water and wastewater treatment, and recently as the latest source of energy biomass for the production of bioethanol.
Lemnaceae wzrastając w warunkach właściwego im oświetlenia nie wymagają specjalnych suplementacji podłoża substancjami stymulującymi wzrost roślin, jednak w przyp adku ich zastosowania, pożywka uzupełnienia jest preparatami humusowymi lub ekstraktem torfowym. Ze względu na złożoność procesów i otrzymanie niejednorodnych danych, brak jest jednoznacznych rezultatów i wymagają one dalszych badań, gdyż otrzymywano różne wyniki zależnie od stosowanych warunków' hodowli. Przykładowo w doświadczeniach wykonanych przez Hutnera et al. (1950, cyt. wg Landolt, 1957. E. Physiologische und okologische Untersuchungen an Lemnaceen. Ber, Schweiz. Bot. Ges., 67) wykazano, że ekstrakt z torfu i ekstrakt nawozowy nie wywierały stymulującego działania na wzrost- i rozwój roślin przynależnych do Lemnaceae (Spirodela oligorrhiza). Z kolei w. pracy Wcisło (1963) (Wcisło H., 1963. Some observations on lines of Lemma trisulca L. grown under aseptic conditions. - Acta biol.Lemnaceae, growing under appropriate lighting conditions, do not require any special supplementation of the substrate with plant growth stimulating substances, but when used, the supplementation medium is made of humic preparations or peat extract. Due to the complexity of the processes and the obtaining of heterogeneous data, there are no unequivocal results and they require further research, as different results were obtained depending on the cultivation conditions used. For example, in the experiments performed by Hutner et al. (1950, quoted after Landolt, 1957. E. Physiologische und okologische Untersuchungen an Lemnaceen. Ber, Schweiz. Bot. Ges., 67) it was shown that peat extract and fertilizer extract did not exert a stimulating effect on the growth and development of related plants to Lemnaceae (Spirodela oligorrhiza). In turn, in Wcisło (1963) (Wcisło H., 1963. Some observations on lines of Lemma trisulca L. grown under aseptic conditions - Acta biol.
Cracoviensia Ser. Botanica 6: 161-179) podczas hodowli roślin wodnych Lemna trisulca na suplementownym medium PfefTera przygotowanym na ekstrakcie torfowym w stężeniu 1%, uzyskano istotnie, wyższą biomasę roślin. Jednak hodowane na wspomnianym podłożu te same rośliny, bez suplementacji składnikami mineralnymi i bez obecności cukrów długo zachowywały żywotność, lecz z kolei bardzo słabo rosły, a suplementacja medium PfefTera 0,04% peptonem: bakteriologicznym spowodowała deformację członów pędowych roślin Lemna trisulca, wyrażające się ich zwijaniem (Wcisło, H.: Some observations on olones of Lemna Iritulca L, grown under aseptic conditions. - Acta Biol. Cracoviensia Ser. Botanica 6: 161-179). Jednym ze sposobów stosowanych dla stymulacji wzrostu makrofitów Lemnaceae jest zastosowania metody przewietrzania pożywki.Cracoviensia Ser. Botanica 6: 161-179) during the cultivation of aquatic plants Lemna trisulca on the PfefTera supplementary medium prepared on the peat extract at a concentration of 1%, a significantly higher plant biomass was obtained. However, the same plants grown on the mentioned medium, without supplementation with minerals and without the presence of sugars, remained viable for a long time, but grew very poorly, and supplementation of the PfefTera medium with a bacteriological peptone of 0.04% caused deformation of the stem members of Lemna trisulca plants, expressed by their curling (Wcisło, H .: Some observations on olones of Lemna Iritulca L, grown under aseptic conditions. - Acta Biol. Cracoviensia Ser. Botanica 6: 161-179). One of the methods used to stimulate the growth of Lemnaceae macrophytes is the use of the medium ventilation method.
Liczne badania dowiodły stymulowanego wzrostu. roślin z rodziny Lemnaceae na różnych podłożach mineralnych suplementowanych znaczną ilością wapnia. Zjawisko to jest znane, ponieważ Lemnaceae , jako rośliny wyższe, posiadają znaczne wymagania w zakresie makroelementów. Nie jest to fakt nieoczekiwany jeśli zważyć, że Lemnaceae należą do roślin wyższych - kwiatowych i wymagają wapnia do swego rozwoju, a także wielu mikroelementów w tym cynku, boru, manganu i molibdenianu. Z tego powodu najczęściej do hodowli stosuje się medium Hutnera (Landolt E., 1957. Physiologische und okologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67), Pirsona i Seidela (Kandeler R., 1955, ϋber die Blutenbildung bei Lemna gibba L. I. Kulturbedingungen und Tageslangen abhangigkeit, Zeit f.: Bot. 43, 61-71.) oraz Hoaglanda (Bonner i Galston 1952, Principles of Plant Physiology, Vol. 115, Issue2995, pp. 580). Medium wg Hutnera (Landolt E 1957. Physiologische und onkologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges.. 67) składa się z wysokiej zawartości mikroelementów, a także Na2EDTA) i (sól dwusodowa kwasu etylenodwuaminocztero-octowego). Ponadto w przypadku medium mineralnego, które jest bardziej alkaliczne (pH powyżej 6), przyswajalność żelaza w formie organicznej (cytrynian lub winian żelazowy) znacznie wzrasta, a dodatkowo suplementacja poprzez EDTA wpływa na zwiększone przyswajanie jonów żelaza w formie soli nieorganicznej. Wieloma badaniami został potwierdzony rezultat, iż najlepszy wzrost, osiągają, rośliny wodne z rodziny Lemnaceae w zakresie pH medium 4.8-6,1, a niewielkie odchylenia od wskazanej wartości nie mają większego znaczenia (Henssen 1954, Landolt E,, 1957. Physiologische und okologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67). W celu polepszenia wzrostu wegetatywnego kultur pożywka suplementowana jest organicznym źródłem węgla, w postaci 1% sacharozy lub fruktozy czy glukozy (Gorham P. R 1945, Growth factor studies with Spirodda polyrrhiza (L.) Schleid. Am. J.Bot. 32, 496-505.; Henssen 1954, Die Dauerorgane von Spirodda polyrrhiza (L.) Schleid, in physiologischer Betrachtung. Flora, 141, 523-566; Kandel er R., 1955, ϋber die Blutenbildung bei Lemna gibba L. I. Kulturbedingungen und Tageslangen abhangigkeit, Zeit f. Bot, 43, 61-71; Czopek 1959, Researches on the physiology of formation and germination of turions in Spirodda polyrrhiza (L.) Schleiden, Acta. Biol. Crac. Ser. Bot. 2, 76-90; Wcisło H,, 1963. Some observations on lines of Lemna trisulca L. grown under aseptic conditions. - Acta Biol. Crac. Ser. Botanica 6: 161-179).Numerous studies have proven stimulated growth. plants of the Lemnaceae family on various mineral substrates supplemented with a significant amount of calcium. This phenomenon is known because Lemnaceae, as higher plants, have significant macronutrient requirements. This is not an unexpected fact considering that Lemnaceae are higher-floral plants and require calcium for their development, as well as many micronutrients, including zinc, boron, manganese and molybdate. For this reason, Hutner's medium is most often used for breeding (Landolt E., 1957. Physiologische und okologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67), Pirson and Seidel (Kandeler R., 1955, ϋber die Blutenbildung bei Lemna gibba LI Kulturbedingungen und Tageslangen abhangigkeit, Zeit f .: Bot. 43, 61-71.) And Hoagland (Bonner and Galston 1952, Principles of Plant Physiology, Vol. 115, Issue2995, pp. 580). The medium according to Hutner (Landolt E 1957. Physiologische und onkologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges .. 67) consists of a high content of microelements as well as Na2EDTA) and (disodium salt of ethylenediaminetetraacetic acid). In addition, in the case of a mineral medium that is more alkaline (pH above 6), the absorption of iron in an organic form (ferric citrate or tartrate) increases significantly, and additionally EDTA supplementation increases the absorption of iron ions in the form of inorganic salt. Many studies have confirmed the result that the best growth is achieved by aquatic plants of the Lemnaceae family in the pH range of the medium 4.8-6.1, and slight deviations from the indicated value are of no great importance (Henssen 1954, Landolt E, 1957. Physiologische und okologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67). In order to improve the vegetative growth of cultures, the medium is supplemented with an organic carbon source in the form of 1% sucrose or fructose or glucose (Gorham P. R 1945, Growth factor studies with Spirodda polyrrhiza (L.) Schleid. Am. J. Bot. 32, 496. -505 .; Henssen 1954, Die Dauerorgane von Spirodda polyrrhiza (L.) Schleid, in physiologischer Betrachtung. Flora, 141, 523-566; Kandel er R., 1955, ϋber die Blutenbildung bei Lemna gibba LI Kulturbedingungen und Tageslangiten, Zeitangigkeiten, f. Bot, 43, 61-71; Czopek 1959, Researches on the physiology of formation and germination of turions in Spirodda polyrrhiza (L.) Schleiden, Acta. Biol. Crac. Ser. Bot. 2, 76-90; Wcisło H ,, 1963. Some observations on lines of Lemna trisulca L. grown under aseptic conditions. - Acta Biol. Crac. Ser. Botanica 6: 161-179).
PL 239 291 B1PL 239 291 B1
Ponadto Landolt wykazał (Landolt E., 1957, Physiologische und okologische Unters uchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67), że rozcieńczenie 10 - krotnie medium wg Hutnera, tylko w niewielkim stopniu powoduje inhibicję wzrostu roślin. Jednak odwrotną reakcję zaobserwowano dla medium wg Pirsona i Seideia (Kandeler R., 1955, ϋber die Blutenbildung bei Lemna gibba L. I. Kulturbedingungen und Tageslangen abhangigkeit, Zeit f. Bot. 43, 61-71) gdzie wystąpiło silne zahamowanie.wzrostu Lemna gibka (Czopek 1959, Cultivation of Polish Lemnaceae species in laboratory conditions. Acta. Biol. Crac. Ser. Bot. 2, 76-90). Ponadto pięciokrotne stężenie pożywki wg Hutnera powoduje zupełne zahamowanie wzrostu obydwu gatunków roślin Lemna i Wolffia. Z kolei badania Wcisło (1963) prowadzone w warunkach aseptycznych nad morfologiczno -fizjologicznymi różnicami w obrębie klonów Lemna trisulca potwierdziły możliwość hodowli roślin na pożywce Hoaglanda i Clarka (The, water-culture method for .growing, plants without soil. Circular, California Agricultural Experiment Station, 347, 1938; Wcisło H., 1963. Some observations on lines of Lemna trisulca L. grown under aseptic conditions. Acta Biol. Crac. Ser. Botanica 6: 161 -179) oraz na zmodyfikowanej pożywce Rhodego i Pfeffera (Wcisło H., 1963. Some observations on lines of Lemna trisulca L. grown under aseptic conditions. Acta biol. Cracoviensia Ser. Botanica 6: 161 -179). Z kolei na medium Hutnera (Landolt E., 1957. Physiologische und okologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67), Knopa (Reski, R., and Abel, W. O. 1985 Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine. Planta 165, 354-358), Pirsona i Seidela (Kandeler R,, 1955, ϋber die Blutenbildung bei Lemna gibba. L. I. Kulturbedingungen und Tageslangen abhangigkeit, Zeit f. Bot, 43,. 61-71) Lemna trisulca wzrastała znacznie wolniej.Moreover, Landolt demonstrated (Landolt E., 1957, Physiologische und okologische Unters uchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67) that diluting 10 times the medium according to Hutner causes only a slight inhibition of plant growth. However, an opposite reaction was observed for the medium according to Pirson and Seidei (Kandeler R., 1955, ϋber die Blutenbildung bei Lemna gibba LI Kulturbedingungen und Tageslangen abhangigkeit, Zeit f. Bot. 43, 61-71) where there was a strong inhibition of growth of Lemna gibka (Czopek 1959, Cultivation of Polish Lemnaceae species in laboratory conditions. Acta. Biol. Crac. Ser. Bot. 2, 76-90). Moreover, the fivefold concentration of the medium according to Hutner causes complete inhibition of the growth of both Lemna and Wolffia plant species. In turn, the research by Wcisło (1963) conducted under aseptic conditions on the morphological and physiological differences within the Lemna trisulca clones confirmed the possibility of growing plants on the Hoagland and Clark's broth (The, water-culture method for .growing, plants without soil. Circular, California Agricultural Experiment. Station, 347, 1938; Wcisło H., 1963. Some observations on lines of Lemna trisulca L. grown under aseptic conditions. Acta Biol. Crac. Ser. Botanica 6: 161-179) and on modified Rhodego and Pfeffer's medium (Wcisło H ., 1963. Some observations on lines of Lemna trisulca L. grown under aseptic conditions. Acta biol. Cracoviensia Ser. Botanica 6: 161-179). In turn, on the medium of Hutner (Landolt E., 1957. Physiologische und okologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges., 67), Knopa (Reski, R., and Abel, WO 1985 Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine. Planta 165, 354-358), Pirsona i Seidela (Kandeler R ,, 1955, ϋber die Blutenbildung bei Lemna gibba. LI Kulturbedingungen und Tageslangen abhangigkeit, Zeit f. Bot, 43 ,. 61 -71) Lemna trisulca grew much more slowly.
Sposób, przygotowań i a mediów do hodowli makrofitów wodnych z rodziny Lemnaceae, według wynalazku polega na tym, że podłoże w postaci roztworu wodnego odcieków po fermentacji beztlenowej towarzyszącej produkcji biogazu o stężeniu suchej substancji 0,1-10% poddaje się ozonowaniu stosując 0,01-10 części wagowych ozonu na 100 części wagowych podłoża, w temperaturze 0-50°C.The method of preparation and a media for the cultivation of aquatic macrophytes from the Lemnaceae family, according to the invention, consists in the fact that the substrate in the form of an aqueous solution of effluents after anaerobic fermentation accompanying the production of biogas with a dry substance concentration of 0.1-10% is subjected to ozonization with 0.01- 10 parts by weight of ozone to 100 parts by weight of substrate at 0-50 ° C.
W innym wariancie według wynalazku ozonowanie prowadzi się przepuszczając przez podłoże mieszaninę tlenu i ozonu.In another variant of the invention, ozonation is carried out by bubbling a mixture of oxygen and ozone through the substrate.
Proces prowadzi się w sposób ciągły lub okresowy.The process is carried out continuously or batchwise.
Sposób przygotowania mediów do hodowli makrofitów wodnych z rodziny Lemnaceae według wynalazku pozwala na uzyskanie podłoża, którego stosowanie zwiększa przyrost’ biomasy-makrofitów wodnych z rodziny Lemnaceae od 50 do 100% w stosunki do tego samego podłoża nie poddanego procesowi ozonowania. Sposób ten nie powoduje generowania inhibitorów-wzrostu makrofitów wodnych z rodziny Lemnaceae. W porównaniu ze znanymi sposobami suplementacji-mediów hodowlanych jest znacznie prostszy. Stosując sposób według wynalazku przyspiesza się wzrost biomasy makrofitów i poprawia jakość finalnej biomasy.The method of preparing media for the cultivation of aquatic macrophytes from the Lemnaceae family according to the invention allows to obtain a substrate, the use of which increases the growth of biomass-aquatic macrophytes from the Lemnaceae family by 50 to 100% in relation to the same substrate not subjected to the ozonation process. This method does not generate water macrophyte growth inhibitors of the Lemnaceae family. Compared to known methods of supplementation-breeding media, it is much simpler. By applying the method according to the invention, the growth of macrophyte biomass is accelerated and the quality of the final biomass is improved.
Sposób według wynalazku ilustruje poniższy przykład.The method according to the invention is illustrated by the following example.
P r z y k ł a dExample
Ozonowaniu poddano wodny odciek po fermentacji beztlenowej towarzyszącej produkcji biogazu z wywarów gorzelniczych o stężeniu suchej substancji 0,1-5%. W tym celu 100 kg odcieku umieszczono w reaktorze, po czym przepuszczano przez ten roztwór mieszaninę tlenu i ozonu zawierającą 80 g ozonu w 1 m3, w temperaturze 20°C. Proces ozonowania zakończono po przepuszczeniu przez odciek 0,25 kg ozonu.Ozonation was applied to the water effluent after anaerobic fermentation accompanying the production of biogas from stillage stillage with a dry substance concentration of 0.1-5%. For this purpose, 100 kg of the effluent was placed in the reactor, and then a mixture of oxygen and ozone containing 80 g of ozone in 1 m 3 was passed through this solution at a temperature of 20 ° C. The ozonation process was completed after 0.25 kg of ozone was passed through the effluent.
Po zakończeniu ozonowania odciek zaszczepiono 1 kg biomasy makrofitów wodnych z rodziny Lemnaceae, Wzrost roślin prowadzono w reaktorze otwartym w ciągu 10 dni. W wyniku przeprowadzonej hodowli odnotowano zwiększony przyrost biomasy roślin o 100% (8 kg) w stosunku do próby kontrolnej (4 kg), którą była analogiczna hodowla na podłożu nie poddanym ozonowaniu.After the end of ozonation, the effluent was inoculated with 1 kg of biomass of aquatic macrophytes from the Lemnaceae family. Plant growth was carried out in an open reactor for 10 days. As a result of the carried out cultivation, an increased increase in plant biomass by 100% (8 kg) was noted in relation to the control sample (4 kg), which was an analogous cultivation on a substrate not subjected to ozonation.
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