PL124241B2 - Method of preparation of medium for production of lyases type pectolytic enzymes - Google Patents
Method of preparation of medium for production of lyases type pectolytic enzymes Download PDFInfo
- Publication number
- PL124241B2 PL124241B2 PL21679779A PL21679779A PL124241B2 PL 124241 B2 PL124241 B2 PL 124241B2 PL 21679779 A PL21679779 A PL 21679779A PL 21679779 A PL21679779 A PL 21679779A PL 124241 B2 PL124241 B2 PL 124241B2
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- PL
- Poland
- Prior art keywords
- medium
- production
- type
- preparation
- water
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 238000000034 method Methods 0.000 title claims description 7
- 102000004190 Enzymes Human genes 0.000 title claims description 6
- 108090000790 Enzymes Proteins 0.000 title claims description 6
- 230000002351 pectolytic effect Effects 0.000 title claims description 4
- 238000002360 preparation method Methods 0.000 title description 4
- 102000004317 Lyases Human genes 0.000 title description 2
- 108090000856 Lyases Proteins 0.000 title description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 10
- 241000335053 Beta vulgaris Species 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 239000011707 mineral Substances 0.000 claims description 4
- 239000008363 phosphate buffer Substances 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 239000002699 waste material Substances 0.000 claims description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Description
Przedmiotem wynalazku jest sposób przygotowywania podloza hodowlanego do produkcji enzymów pek- tolitycznych typu liaz.Znane sa technologie przygotowywania podlozy produkcyjnych z róznych odpadów przemyslu spozyw¬ czego jak na przyklad wyslodki buraczane, wytloki jablkowe i inne, które zawiesza sie w wodzie i po sterylizacji stosuje sie do hodowli.Istota wynalazku jest sposób przygotowywania podloza hodowlanego do produkcji enzymów pektolitycz- nych typu liaz z odpadów przemyslu cukrowniczego, w którym okreslona ilosc wyslodków buraczanych rozdrab¬ nia sie a nastepnie zawiesza sie w wodzie, charakteryzujacy sie w tym, ze zawieszone w wodzie wyslodki buraczane przetrzymuje sie w autoklawie w temperaturze 90° - 110°C przez 20 do 40 minut, a nastepnie filtruje sie i ustala stezenie substancji wyciagowych tak, aby wynosilo od 2% do 8%, po czym wzbogaca sie wyciag w sole mineralne oraz dodaje sie bufor fosforanowy opH=6,0 w takiej ilosci, aby stezenie koncowe buforu w podlozu wynosilo 0,05 M.Przez stosowanie wynalazku otrzymuje sie roztwory o dowolnym stezeniu substancji wyciagowych, co jest wazne z tego wzgledu, ze stezenie wyciagu ma istotny wplyw na wydajnosc produkcji enzymów. Wedlug tego sposobu mozna podwyzszyc zawartosc substancji wyciagowych z wyslodków buraczanych w podlozu hodowla¬ nym od 2% do 8%, co nie jest mozliwe w metodach tradycyjnych. Poza tym, podloza wedlug wynalazku latwo oczyszczaja sie po hodowli i nie zawieraja resztek wyslodków buraczanych, co ma istotne znaczenie przy dalszej obróbce plynu w celu uzyskania preparatów enzymatycznych.Przyklad: 40 g wyslodków buraczanych po zmieleniu w mlynie kulkowym zawiesza sie w wodzie i przetrzymuje sie w autoklawie przez 30 min. w temperaturze 100°C. Otrzymana rozparowana mase wyciska sie przez plótno. Stezenie substancji wyciagowych w plynie ustala sie poprzez odparowanie wody lub rozcienczanie woda. Po ustaleniu gestosci wyciagu na 4% dodaje sie sole mineralne i nastepnie wprowadza sie bufor fosforano¬ wy o pH=6,0 w takiej ilosci, by stezenie koncowe buforu w podlozu wynosilo 0,05 M.2 124 241 Zastrzezenie patentowe Sposób przygotowywania podloza hodowlanego do produkqi enzymów pektolitycznych typu liaz z odpa¬ dów przemyslu cukrowniczbgo, w którym okreslona ilosc wyslodków buraczanych rozdrabnia sie, a nastepnie zawiesza sie w wodzie, znamienny tym, ze zawieszone w wodzie wyslodki buraczane przetrzymuje sie w autoklawie w temperaturze 90* — 110°C przez 20 do 40 minut, a nastepnie filtruje sie i ustala stezenie sub¬ stancji wyciagowych tak, aby wynosilo od 2% do 8%, po czym wzbogaca sie wyciag w sole mineralne oraz dodaje sie bufor fosforanowy o pH=6,0, w takiej ilosci, aby stezenie koncowe buforu w podlozu wynosilo 0,05 M.PracowniaPoligraficznaUP PRL. Naklad 100egz.Cena 100 zl PLThe subject of the invention is a method of preparing a culture medium for the production of pactolytic enzymes of the lyase type. There are known technologies for the preparation of production substrates from various wastes of the food industry, such as beet pulp, apple pomace and others, which are suspended in water and used after sterilization. The essence of the invention is a method for the preparation of a culture medium for the production of pectolytic enzymes of the sugar industry type, in which a certain amount of beet pulp is crushed and then suspended in water, characterized by the fact that the Beets are kept in an autoclave at 90 ° - 110 ° C for 20 to 40 minutes, then filtered and the concentration of the extract substances is adjusted to 2% to 8%, then the extract is enriched with mineral salts and added phosphate buffer opH = 6.0 in such an amount that the final buffer concentration in the medium is 0.05 M. By using the invention, solutions with any concentration of extracting substances are obtained, which is important because the concentration of the extract has a significant impact on the enzyme production efficiency. According to this method, it is possible to increase the content of beet pulp extract in the cultivation medium by 2% to 8%, which is not possible with traditional methods. In addition, the substrate according to the invention is easily cleaned after cultivation and does not contain beet pulp residues, which is important for the further treatment of the liquid to obtain enzymatic preparations. Example: 40 g of beet pulp after grinding in a ball mill, are suspended in water and stored in an autoclave for 30 minutes. at 100 ° C. The resulting vaporized mass is squeezed through the canvas. The concentration of the extracts in the liquid is established by evaporating the water or by diluting it with water. After adjusting the density of the extract to 4%, the mineral salts are added and then the phosphate buffer of pH = 6.0 is introduced in such an amount that the final concentration of the buffer in the medium is 0.05 M. 2 124 241 Patent claim Method of preparing the culture medium for the production of pectolytic enzymes of the lye type from waste from the sugar industry, in which a certain amount of beet pulp is crushed and then suspended in water, characterized by the fact that the beet pulp suspended in water is kept in an autoclave at a temperature of 90 * - 110 ° C for 20 to 40 minutes, and then filtering and adjusting the concentration of the extracts to be from 2% to 8%, after which the extract is enriched with mineral salts and a phosphate buffer with pH = 6.0 is added, such the amount so that the final concentration of the buffer in the substrate was 0.05 M. PracowniaPoligraficznaUP PRL. Mintage 100 copies. Price PLN 100 PL
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL21679779A PL124241B2 (en) | 1979-06-29 | 1979-06-29 | Method of preparation of medium for production of lyases type pectolytic enzymes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL21679779A PL124241B2 (en) | 1979-06-29 | 1979-06-29 | Method of preparation of medium for production of lyases type pectolytic enzymes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL216797A2 PL216797A2 (en) | 1980-08-11 |
| PL124241B2 true PL124241B2 (en) | 1983-01-31 |
Family
ID=19997224
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL21679779A PL124241B2 (en) | 1979-06-29 | 1979-06-29 | Method of preparation of medium for production of lyases type pectolytic enzymes |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL124241B2 (en) |
-
1979
- 1979-06-29 PL PL21679779A patent/PL124241B2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL216797A2 (en) | 1980-08-11 |
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