PH12017000246A1 - Composition and method of producing a multi-functional biofertilizer for use as seed/planting material inoculant for use in all crops - Google Patents
Composition and method of producing a multi-functional biofertilizer for use as seed/planting material inoculant for use in all crops Download PDFInfo
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- PH12017000246A1 PH12017000246A1 PH12017000246A PH12017000246A PH12017000246A1 PH 12017000246 A1 PH12017000246 A1 PH 12017000246A1 PH 12017000246 A PH12017000246 A PH 12017000246A PH 12017000246 A PH12017000246 A PH 12017000246A PH 12017000246 A1 PH12017000246 A1 PH 12017000246A1
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- glomus
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000002054 inoculum Substances 0.000 title claims abstract description 13
- 239000000463 material Substances 0.000 title claims abstract description 12
- 239000000203 mixture Substances 0.000 title claims abstract description 8
- 239000002689 soil Substances 0.000 claims abstract description 20
- 241000894007 species Species 0.000 claims abstract description 19
- 230000000813 microbial effect Effects 0.000 claims abstract description 13
- 244000005700 microbiome Species 0.000 claims abstract description 10
- 241000233866 Fungi Species 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000011574 phosphorus Substances 0.000 claims abstract description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims description 14
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 239000011785 micronutrient Substances 0.000 claims description 6
- 235000013369 micronutrients Nutrition 0.000 claims description 6
- 241000235502 Gigaspora margarita Species 0.000 claims description 5
- 241001295761 Paraglomus occultum Species 0.000 claims description 5
- 239000003610 charcoal Substances 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- 241000235503 Glomus Species 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 239000002361 compost Substances 0.000 claims description 4
- 230000002538 fungal effect Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 239000004576 sand Substances 0.000 claims description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 4
- 240000002900 Arthrospira platensis Species 0.000 claims description 3
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 3
- 241000192700 Cyanobacteria Species 0.000 claims description 3
- 241001123592 Gigaspora albida Species 0.000 claims description 3
- 241000984804 Glomus aggregatum Species 0.000 claims description 3
- 241000849146 Ochrobactrum ciceri Species 0.000 claims description 3
- 241001051694 Ochrobactrum pseudogrignonense Species 0.000 claims description 3
- 241000592795 Paenibacillus sp. Species 0.000 claims description 3
- 241000358085 Rhizophagus clarus Species 0.000 claims description 3
- 241000168282 Rhizophagus manihotis Species 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000000356 contaminant Substances 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 235000021073 macronutrients Nutrition 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 239000004606 Fillers/Extenders Substances 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 235000019270 ammonium chloride Nutrition 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 239000000122 growth hormone Substances 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 239000004317 sodium nitrate Substances 0.000 claims description 2
- 235000010344 sodium nitrate Nutrition 0.000 claims description 2
- 229910021654 trace metal Inorganic materials 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 210000003278 egg shell Anatomy 0.000 claims 3
- 238000003306 harvesting Methods 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 241001495180 Arthrospira Species 0.000 claims 1
- 235000016425 Arthrospira platensis Nutrition 0.000 claims 1
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 102000018997 Growth Hormone Human genes 0.000 claims 1
- 108010051696 Growth Hormone Proteins 0.000 claims 1
- 238000007605 air drying Methods 0.000 claims 1
- 229940011019 arthrospira platensis Drugs 0.000 claims 1
- 239000000969 carrier Substances 0.000 claims 1
- 238000003801 milling Methods 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 230000012010 growth Effects 0.000 abstract description 5
- 229930191978 Gibberellin Natural products 0.000 abstract description 3
- 239000003337 fertilizer Substances 0.000 abstract description 3
- 238000009472 formulation Methods 0.000 abstract description 3
- 239000003448 gibberellin Substances 0.000 abstract description 3
- 229930192334 Auxin Natural products 0.000 abstract description 2
- 239000002363 auxin Substances 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 abstract description 2
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 abstract description 2
- 244000000034 soilborne pathogen Species 0.000 abstract description 2
- 238000004178 biological nitrogen fixation Methods 0.000 abstract 1
- 239000003630 growth substance Substances 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000021749 root development Effects 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 230000007226 seed germination Effects 0.000 abstract 1
- 230000035040 seed growth Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 abstract 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000035764 nutrition Effects 0.000 description 5
- 230000035558 fertility Effects 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 230000007483 microbial process Effects 0.000 description 1
- 235000021232 nutrient availability Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention involves the method of producing biological fertilizer of biofertilizer or microbial inoculant that can be applied to seeds, planting materials or to soil to enhance seed germination, growth and yield. The biofertilizer is multi-functional in nature, comprising of many species and strains of microorganisms that have beneficial traits such as but not limited to biological nitrogen fixation, solubilisation of unavailable phosphorus, secretion of growth regulators, i.e. auxins and gibberellins. The biofertilizer formulations also contain spores and mycelia of endomychorrhizal fungi which has known growth promoting properties. Through synergistic action of the beneficial microorganisms, the plant has greater access to water and nutrients, better root development, greater resistance, to soil-borne pathogens and enhance flowering and fruiting, thereby leading to better crop yield. This biofertilizer formulation is for use in soil fertilization, enhancement of soil biodiversity and reclamation of degraded lands
Description
Method of Producing a Multi-functional Biofertilizer for Use as Seed/Planting <
Material Inoculant for Use in All Crops =
The present invention relates in general to agricultural farm input used for = growing crops but more particularly as a fertilizer, specifically a biofertilizer or a © microbial inoculant that is applied to seeds, planting materials or soil prior to sowing/planting. The invention focuses on the method of producing the biofertilizer. ic
The present invention relates to microbial preparation that can be used for crop production and to bring back soil fertility and biodiversity in agricultural fields. It is a known fact that man has used repeatedly large volume of synthetic chemical fertilizers to enhance soil fertility and increase yield. However, through time, soil properties deteriorated, with increasing soil acidity, toxicity of micronutrients, deficiency of macronutrients and death of majority of resident macro and microorganisms. The present invention provides a means of bringing back soil fertility and biodiversity, since this invention contains many species and strains of microbes that are known to be beneficial to plant through provision of nutrient elements from the air, and unavailable sources. Furthermore, the beneficial microbes in particular the endomycorrhizal fungi, serves as root extenders in the greater accumulation of water and nutrients as well as protection from soil-borne pathogens. /
Bioferiilizer or aiso referred to as microbial inoculant is not a recent technology.
Biofertilizers are microbial preparations containing live or latent cells of efficient - strains of microorganisms, which could be nitrogen fixing, phosphate solubilizing or — cellulose degrading. These are for application to seed, soil or composting materials. os
The major aim in using biofertilizers is to increase the numbers of such . microorganisms and accelerate certain microbial processes. Through microbial action 5 nutrient availability and plant assimilation are hastened. =
Earlier biofertilizer technologies contain only one or few strains or species or type of microbes and targeted to be applied to specific crops, such as the prior art
WO/2013/098829 which includes only mycorrhizal species. Another prior art
WO2008/130701 contains only psychrophilic bacterial species, while prior art [PC
C05G3/04 for a microbial inoculant contains probiotics, yeasts and two bacterial species namely Bacillus licheniformis and Bacillus subtilis for rice and com (CN104402650). Similarly, another prior art CO5F11/08 for a biofertilizer product consists of a different set of bacterial species, namely: Bacillus megaterium and
Bacillus mucilagenosus for agricultural fertilization. Although the biofertilizer products and formulations seem effective due to the action of the species and strains of microbes present, it cannot be denied that their combined action maybe better.
In seedling establishment, the new plant requires nutrient for its continuous growth. If microbial inoculant applied is inadequate of nutrients, the microbes compete with the plant for the nutrition in the rhizosphere, and if really inadequate, the plant suffers, leading to stunted or poor growth. To correct this problem, biofertilizers should be supplied with enough nutrition for the microbes to maintain their population while in storage and when applied to the soil/plant. The present - invention made sure that the microbial components are supplied with adequate = nutrition in a readily available and organic form such as fresh growth medium and i» microbial biomass. However, a prior art exist with this nature. Prior arts C05D9/00 - and COSF11/08 refer to the method of producing a bio-stabilized biofertilizer in ~ pelleted form. These prior arts also contain microbial inoculant but are applied to ® make compost. ~
The present invention tries to solve the deficiencies or limitations of the prior arts by being non-host specific, bio-stabilized with a ready source of nutrients and multitude of reserved nutrition in the form of microbial biomass in the compost : component of the carrier, with mixed microorganisms not only of mycorrhizal fungi but aiso of many beneficial bacteria and fungi. These microbes has the capability to fix atmospheric nitrogen, solubilize unavailable phosphorus as well as secrete plant growth regulators, e.g. auxin and gibberellins.
The mycorrhizal fungi in the biofertilizer consist of the following cultured species, namely: Glomus manihotis, Gl. fasciculatum, Gl. etunicatum, Paraglomus occultum, Glomus clarum, Glomus aggregatum, Gigaspora margarita and Gigaspora albida which could be present in various proportions.
The biofertilizer also consists of the following bacterial species, namely:
Bacillus sp., Paenibacillus sp., Ochrobactrum pseudogrignonense, Ochrobactrum ciceri and Stenotrophomas pavanii. The biofertilizer also contains the cyanobacterial species Arthrospira platensis. The present invention also contains sources of macro gi
EP and micro nutrients which can be used by the microbe and the developing root in the = early stages of growth. = foment
The present invention comes in powder form and can easily be used to coat seeds or planting material. Itis slightly soluble in water and hence can be applied as - a slurry mixture to coat seeds and planting materials. -
Fd
The present invention is a multi-functional biofertilizer for use by all crops, either applied onto seeds, cuttings, tubers or other planting materials. The biofertilizer is in a stabilize form and is more complete compared with single strain inoculants, since it has many species and strains of microorganisms, ready source of nutrients for their growth and the developing root. The biofertilizer is composed of many species and strains of microorganisms such as mycorrhizal fungi, namely
Glomus manihotis, Gl. fasciculatum, Gl. etunicatum, Paraglomus occultum, Gl. clarum, Gl. aggregatum, Gigaspora margarita and Gi. albida. The biofertilizer also contains the following bacterial species, namely: Bacillus sp., Paenibacillus sp.,
Ochrobactrum pseudogrignonense, Ochrobactrum ciceri and Stenotrophomas pavanii. The biofertilizer also contains the cyanobacterial species Arthrospira platensis. These microbes has earlier been screened for their ability to fix atmospheric nitrogen, solubilized phosphorus, secrete growth hormones such as
Indole-Acetic-Acid (IAA), gibberellin and serve as source of other nutrients for the nutrition of the other microbes and developing plant. yf
Fa ey)
The method of producing the present invention includes the following steps: ~ 1. Preparation of the carrier medium: w 2. Preparation of bacterial strains; 3. Preparation of mycorrhizal fungi; = 4. Preparation of cyanobacterial species; and - 5. Mixing and packing of components
For the preparation of the carrier, a volume to volume ratio of 2:1:1 for soil, compost and charcoal are used. All components were individually air dried, and pulverized in a hammer mil before blending together. The said carrier mixture was individually packed in plastic bags before autoclaving for an hour at 15 psi for three consecutive days, then cooled prior to use.
For the preparation of bacterial inoculants, each strain were mass produced in liquid medium comprising of mallic acid, potassium hydroxide, yeast extract, ammonium chloride and micronutrient solution for 48 hours at ambient temperature with regular shaking. The bacterial strains were harvested and stored at 4 °C until ready for use.
For the preparation of cyanobacteria cultures (drthrospira platensis), they were grown in liquid medium comprising of sodium nitrate, di potassium phosphate, magnesium sulfate, calcium chloride, citric acid and trace metal mix, then exposed to light until use.
ee rs
SE
For the preparation of the mycorrhizal inoculants, the fungal strains (Glomus . manihotis, GI. fasciculatum, Gl. etunicatum, Paraglomus occultum, Glomus clarum, =
Glomus aggregatum, Gigaspora margarita and Gigaspora albida) were mass ~ produced in sterilized sand and soil growth medium consisting of 1:2 ratios by - volume. Grasses like Eleusnie indica used as host plants which are grown for a v period of 5-6 months. The roots, sand and soil growth medium are harvested by © cutting, chopping and dry sieving to remove large particles. To collect the - mycorrhizal fungal spores, the harvested materials are suspended in water, stirred and heavy soil particles are allowed io setiie while passing the solution in series of mesh sieves to recover the mycorrhizal spores. The collected spores are further subjected to sucrose centrifugation to remove contaminants and concentrate the spores.
The individual components are combined together, properly mixed then sealed in a polypropylene plastic bag. (
Claims (11)
- Ce —————————————————— CLAIMS = What is claimed is: -I. A process of preparing a multi-functional biofertilizer comprising of ~ many species and strains of microorganisms, wherein these species are i» composed of mycorrhizal fungi (Glomus mannihotis, Gl. fasciculatum, _Gl. etunicatum, Paraglomus occultum, Glomus claruma, Glomus > aggregatum, Gigaspora margarita, Gi. albida), bacterial species, - namely: Bacillus sp., Paenibacillus sp., Ochrobactrum pseudogrignonense, Ochrobactrum ciceri and Stenotrophomas pavanii. cyanobacteria (Arthrospira platensis) with sources of macro and micro nutrients.
- 2. A process of preparing a multi-functional biofertilizer in claim 1 wherein the species are composed of nitrogen fixers, phosphorus solubilizers, and growth hormone secreting microbes.
- 3. A process of preparing a multi-functional biofertilizer in claim 1 wherein the sources of macro and micro nutrient elements are from sterilized composted material which includes microbial mass, egg shells and charcoal.
- 4. A process of preparing a multi-functional biofertilizer in claim 1 wherein the carriers used are soil, charcoal and compost at a proportion of 2:1:1 on volume by volume basis.F he
- 5. A process of preparing a multi-functional biofertilizer in claim 1 = wherein the process involves blending the different chosen = microorganisms based on initial screening for the trait, purifying and roemte mass producing each species. i
- 6. A process of preparing a multi-functional biofertilizer in claim 1 Le wherein the process includes the recovery the mycorrhizal fungi aside = from the regular wet sieving and decanting technique. o
- 7. A process of preparing a multi-functional biofertilizer in claim 1 wherein the process includes an additional step of sucrose centrifugation to recover mycorrhizal spores and hyphae devoid of soil and other contaminants.
- 8. A process of preparing a multi-functional biofertilizer in claim 1 wherein the preparation of bacterial inoculants involves the followings steps: Mass production of each strain in liquid medium comprising of mallic acid, potassium hydroxide, yeast extract, ammonium chloride and micronutrient solution for 48 hours at ambient temperature with regular shaking; and Harvesting of the strains and storing them at 4° C until ready for use.
- o 9. A process of preparing a multi-functional biofertilizer in claim 1+ wherein the preparation of the cyanobacteria cultures (Arthrospira =, o Dlatensis) involves the following steps: - = Growing of the cultures in liquid medium comprising of - ov 5 sodium nitrate, di potassium phosphate, magnesium sulfate, = - calcium chloride, citric acid and trace metal mix; and w . nd : Exposing the said cultures to light until use.
- 10. A process of preparing a multi-functional biofertilizer in claim 1 wherein the preparation of the mycorrhizal inoculants involves the following steps: Mass production of the fungal strains (Glomus manihotis, Gl. Jasciculatum, Gl. etunicatum, Paraglomus occultum, Glomus clarum, Glomus aggregatum, Gigaspora margarita and Gigaspora albida) in sterilized sand and soil growth medium consisting of 1:2 ratio by volume; Growing of grass and omamental plants to be used as host plants for a period of 5-6 months; Harvesting of roots, sand and soil growth medium through cutting, chopping and dry sieving to remove large particles; Collecting the mycorrhizal fungal spores in which the harvested materials are suspended in water, stirred and the heavy soil particles are allowed to settle while passing the solution in = series of mesh sieves to recover the mycorrhizal spores; and =o Subjecting the collected spores to sucrose centrifugation step to ~ remove contaminants and concentrate the spores and hyphae. =
- 11. A process of preparing a multi-functional biofertilizer in claim 1 o wherein the preparation of extender materials includes the following = steps: = Collecting, air drying and milling of eggshells and charcoal to - transform it to powder form; and Autoclaving the powdered eggshells and charcoal for 15 minutes at 15 psi. fo
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PH12017000246A PH12017000246A1 (en) | 2017-08-30 | 2017-08-30 | Composition and method of producing a multi-functional biofertilizer for use as seed/planting material inoculant for use in all crops |
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| Application Number | Priority Date | Filing Date | Title |
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| PH12017000246A PH12017000246A1 (en) | 2017-08-30 | 2017-08-30 | Composition and method of producing a multi-functional biofertilizer for use as seed/planting material inoculant for use in all crops |
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| PH12017000246A1 true PH12017000246A1 (en) | 2019-03-04 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4294037A (en) * | 1980-02-13 | 1981-10-13 | National Research Development Corporation | Production of mycorrhizal fungi |
| US20100300166A1 (en) * | 2007-04-30 | 2010-12-02 | Mena Campos Jesus | Biofertilizer composition |
| US20150040629A1 (en) * | 2011-12-30 | 2015-02-12 | The Energy And Resources Institute (Teri) | Novel Mycorrhizae-based Biofertilizer Compositions & Method for mass production & formulations of Same |
| US20170174578A1 (en) * | 2011-06-06 | 2017-06-22 | Cool Planet Energy Systems, Inc. | Biochar as a microbial carrier |
| WO2017131821A1 (en) * | 2016-01-29 | 2017-08-03 | Agrinos AS | Microbial consortia |
-
2017
- 2017-08-30 PH PH12017000246A patent/PH12017000246A1/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4294037A (en) * | 1980-02-13 | 1981-10-13 | National Research Development Corporation | Production of mycorrhizal fungi |
| US20100300166A1 (en) * | 2007-04-30 | 2010-12-02 | Mena Campos Jesus | Biofertilizer composition |
| US20170174578A1 (en) * | 2011-06-06 | 2017-06-22 | Cool Planet Energy Systems, Inc. | Biochar as a microbial carrier |
| US20150040629A1 (en) * | 2011-12-30 | 2015-02-12 | The Energy And Resources Institute (Teri) | Novel Mycorrhizae-based Biofertilizer Compositions & Method for mass production & formulations of Same |
| WO2017131821A1 (en) * | 2016-01-29 | 2017-08-03 | Agrinos AS | Microbial consortia |
Non-Patent Citations (3)
| Title |
|---|
| BASHAN, Yoav et. al,"Inoculant Preparation and Formulations for Azospirillum spp.". In: Cassán F., Okon Y., Creus C. (eds) Handbook for Azospirillum. Springer, Cham * |
| de CASTRO, Gabriela Fernandes Pepe da Silva et. al, "Biomass production by Arthrospira platensis under different culture conditions". Food Sci. Technol, Campinas, 35(1): 18-24, Jan.-Mar. 2015 * |
| RIZVI, Rose et.al, "A farmer friendly and economic IPM strategy to combat root-knot nematodes infesting lentil". Cogent Food & Agriculture (2015), 1: 1053214 * |
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