PH12014000009A1 - Molecular diagnostics for white spot virus for prawns and other crustaceans - Google Patents
Molecular diagnostics for white spot virus for prawns and other crustaceansInfo
- Publication number
- PH12014000009A1 PH12014000009A1 PH12014000009A PH12014000009A PH12014000009A1 PH 12014000009 A1 PH12014000009 A1 PH 12014000009A1 PH 12014000009 A PH12014000009 A PH 12014000009A PH 12014000009 A PH12014000009 A PH 12014000009A PH 12014000009 A1 PH12014000009 A1 PH 12014000009A1
- Authority
- PH
- Philippines
- Prior art keywords
- lamp
- wssv
- philippines
- virus
- shrimp
- Prior art date
Links
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
We present an invention that aims to detect geographical strains of white spot syndrome virus in the Philippines using loop-mediated isothermal amplification (LAMP). The primers are specific to local viral strains that are endemic in the Philippines that may spread to other places. The optimum time and temperature conditions for LAMP were 60 min and 65 oC, respectively. The assay was highly specific for WSSV and did not cross-react with other shrimp viruses including monodon baculovirus (MBV), infectious hypodermal and hematopoietic necrosis virus (IHHNV) and hepatopancreatic parvovirus (HPV). The limit of detection of the WSSV using the LAMP assay was 5 pg of DNA ml-1 or 5 fg of the genomic DNA per LAMP reaction and was 10 times more sensitive than conventional PCR in detecting the viral pathogen from infected samples. In vivo, both LAMP and conventional PCR were able to detect the virus from tissues of infected shrimp postlarvae. Our results demonstrated that LAMP is a simple and sensitive method that has potential application as a diagnostic tool in the field for routine detection of WSSV in shrimp aquaculture in the Philippines.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PH12014000009A PH12014000009A1 (en) | 2013-12-27 | 2013-12-27 | Molecular diagnostics for white spot virus for prawns and other crustaceans |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PH12014000009A PH12014000009A1 (en) | 2013-12-27 | 2013-12-27 | Molecular diagnostics for white spot virus for prawns and other crustaceans |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PH12014000009A1 true PH12014000009A1 (en) | 2015-06-29 |
Family
ID=53773042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PH12014000009A PH12014000009A1 (en) | 2013-12-27 | 2013-12-27 | Molecular diagnostics for white spot virus for prawns and other crustaceans |
Country Status (1)
| Country | Link |
|---|---|
| PH (1) | PH12014000009A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107868849A (en) * | 2017-12-01 | 2018-04-03 | 福建省水产研究所（福建水产病害防治中心） | Detect primer sets and its application of prawn IHHNV viruses |
-
2013
- 2013-12-27 PH PH12014000009A patent/PH12014000009A1/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107868849A (en) * | 2017-12-01 | 2018-04-03 | 福建省水产研究所（福建水产病害防治中心） | Detect primer sets and its application of prawn IHHNV viruses |
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