KR20190063512A - Method and kit for diagnosis coronavirus using real-time PCR - Google Patents

Method and kit for diagnosis coronavirus using real-time PCR Download PDF

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KR20190063512A
KR20190063512A KR1020170162353A KR20170162353A KR20190063512A KR 20190063512 A KR20190063512 A KR 20190063512A KR 1020170162353 A KR1020170162353 A KR 1020170162353A KR 20170162353 A KR20170162353 A KR 20170162353A KR 20190063512 A KR20190063512 A KR 20190063512A
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coronavirus
real
time pcr
kit
present
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KR1020170162353A
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윤현규
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윤현규
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The present invention relates to an assay method and a kit for detecting a coronavirus using real-time PCR. The present invention is a method for quantitatively confirming the presence of a coronavirus by selectively amplifying the N gene of the coronavirus genome and detecting fluorescence generated when the fluorescent probe hybridized in an amplification process is decomposed.

Description

리얼타임 PCR을 이용한 코로나바이러스 검사 키트 및 방법 {Method and kit for diagnosis coronavirus using real-time PCR}Technical Field [0001] The present invention relates to a method and a kit for detecting coronavirus using real-time PCR,

본 발명은 리얼타임 PCR을 이용하여 코로나바이러스를 검출하는 키트 및 방법에 관한 것으로 보사 상세하게는 검출대상이 되는 코로나바이러스를 다른 바이러스와 교차반응이 없이 간단하면서도 신속하게 검사하는 키트 및 방법에 관한 것이다. The present invention relates to kits and methods for detecting coronavirus using real-time PCR, and more particularly, to kits and methods for simply and rapidly inspecting a coronavirus to be detected without cross-reacting with other viruses .

코로나바이러스는 다양한 숙주를 감염시킬 수 있으며 각각의 숙주에 특이적이다. 하지만 일부 코로나바이러스의 경우 종범위를 벗어나 다른 종을 숙주로 증식할 수 있으며, 새로운 숙주는 해당하는 코로나바이러스에 대한 면역이 이루어지지 않아 효과적으로 방어를 하지 못해 큰 문제를 야기할 수 있다. Coronaviruses can infect a variety of hosts and are specific for each host. However, some coronaviruses can propagate to other host species outside of the species range, and new hosts can cause serious problems because they can not effectively defend against the corresponding coronaviruses.

최근 SARA, MERS 등 신종 코로나바이러스가 사람에게 감염되어 큰 문제가 발생하여 이러한 신종 코로나바이러스에 대한 적절한 진단법이 개발되어야 한다.Recently, new diagnostic methods for these new coronaviruses should be developed due to the serious problems caused by infection with new corona viruses such as SARA and MERS.

본 발명은 리얼타임 PCR을 이용하여 검출대상이 되는 코로나바이러스를 다른 바이러스와 미생물과 교차반응이 없이 간단하면서도 신속하게 검사하는 키트 및 방법을 제공하는데 그 목적이 있다. It is an object of the present invention to provide a kit and method for simply and rapidly inspecting a coronavirus to be detected using real-time PCR without cross-reacting with other viruses and microorganisms.

상기한 발명의 기술적인 과제를 해결하기 위해 연구한 결과 본 발명자는 리얼타임 PCR을 이용하여 코로나바이러스 N 유전자를 검출하는 검사법을 제공하여 효과적으로 코로나바이러스를 검출하는 것으로 목적을 이룰 수 있다. In order to solve the technical problem of the present invention, the inventors of the present invention have achieved an object of effectively detecting a coronavirus by providing a test method for detecting a coronavirus N gene using real-time PCR.

본 발명에 따르면, 리얼타임 PCR을 이용하여 검출되는 대상인 코로나바이러스를 정확하게 정량적으로 확인을 할 수 있고, 이로써 간단하고도 신속 정확하게 코로나바이러스의 존재 유무를 확인할 수 있다.According to the present invention, it is possible to accurately and quantitatively confirm coronavirus, which is a target to be detected using real-time PCR, so that the presence or absence of corona virus can be confirmed simply and quickly.

도 1은 코로나바이러스의 형태학적, 유전적 구조를 나타내고 있다.Figure 1 shows the morphological and genetic structure of coronaviruses.

이하, 본 발명에 대해 상세하게 기술한다. 코로나바이러스 유전체 중 N 유전자를 표적으로 하여 한 쌍의 특이적인 프라이머로 PCR 방법을 이용하여 증폭하고 중폭된 산물에 교잡화하는 특이적인 형광프로브를 반응에 참여함으로써 증폭과정에서 교잡화된 프로브가 중합효소에 의해 분해되어 여기 형광이 발생하여 검출한다. Hereinafter, the present invention will be described in detail. A specific fluorescent probe that amplifies the N gene of the corona virus genome using PCR as a pair of specific primers and hybridizes to the heavy product is involved in the reaction so that the hybridized probe is amplified by polymerase And excitation fluorescence is generated and detected.

Claims (1)

리얼타임 PCR을 이용한 코로나바이러스 검사 키트로서,
한 쌍의 프라이머를 이용하여 N 유전자를 증폭하고, 하나의 형광프로브가 증폭산물과 교잡화하여 분해될 때 형광값을 확인하는 방법을 이용한 검사방법.As a corona virus test kit using real-time PCR,
Amplification of the N gene using a pair of primers, and hybridization of one fluorescent probe with the amplification product to confirm the fluorescence value when degraded.
KR1020170162353A 2017-11-30 2017-11-30 Method and kit for diagnosis coronavirus using real-time PCR KR20190063512A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102236568B1 (en) 2020-07-07 2021-04-19 주식회사 쉐어플러스 The uncontact specimen collection system to use smart device
KR102247756B1 (en) 2020-08-20 2021-06-03 주식회사 쉐어플러스 walk-through screening clinic with smart function
KR102264483B1 (en) 2020-07-07 2021-06-17 주식회사 쉐어플러스 The movable uncontact specimen collection booth
KR102267326B1 (en) 2020-03-24 2021-06-22 주식회사 젠큐릭스 Method and kit for detecting coronavirus
KR102295044B1 (en) 2021-03-03 2021-08-27 (주)젠에이치플러스 Mask for collecting non-face-to-face virus and method for collecting non-face-to-face virus using the same
KR102295049B1 (en) 2021-03-03 2021-08-27 (주)젠에이치플러스 Non-face-to-face virus collection mask and method to measure the presence or absence of virus infection using it
KR20220102595A (en) 2021-01-13 2022-07-20 에이비온 주식회사 Prophylactic administration for respiratory virus comprising administrating interferon beta to a subject potentially infected by respiratory virus

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102267326B1 (en) 2020-03-24 2021-06-22 주식회사 젠큐릭스 Method and kit for detecting coronavirus
KR102236568B1 (en) 2020-07-07 2021-04-19 주식회사 쉐어플러스 The uncontact specimen collection system to use smart device
KR102264483B1 (en) 2020-07-07 2021-06-17 주식회사 쉐어플러스 The movable uncontact specimen collection booth
KR102247756B1 (en) 2020-08-20 2021-06-03 주식회사 쉐어플러스 walk-through screening clinic with smart function
KR20220102595A (en) 2021-01-13 2022-07-20 에이비온 주식회사 Prophylactic administration for respiratory virus comprising administrating interferon beta to a subject potentially infected by respiratory virus
WO2022154542A1 (en) 2021-01-13 2022-07-21 에이비온 주식회사 Prophylactic administration method against respiratory virus, comprising administering interferon-beta to potential respiratory virus-infected subject
KR102295044B1 (en) 2021-03-03 2021-08-27 (주)젠에이치플러스 Mask for collecting non-face-to-face virus and method for collecting non-face-to-face virus using the same
KR102295049B1 (en) 2021-03-03 2021-08-27 (주)젠에이치플러스 Non-face-to-face virus collection mask and method to measure the presence or absence of virus infection using it

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